Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online February 27, 2003; 10.1104/pp.016113

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
131/3/1239    most recent
pp.016113v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xiao, F.
Right arrow Articles by Zhou, J.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xiao, F.
Right arrow Articles by Zhou, J.-M.
Agricola
Right arrow Articles by Xiao, F.
Right arrow Articles by Zhou, J.-M.

Plant Physiol, March 2003, Vol. 131, pp. 1239-1249

Pto Mutants Differentially Activate Prf-Dependent, avrPto-Independent Resistance and Gene-for-
Gene Resistance1

Fangming Xiao, Ming Lu, Jianxiong Li, Tiehan Zhao, Seung Young Yi, Venkatappa K. Thara, Xiaoyan Tang, and Jian-Min Zhou*

Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506

Pto confers disease resistance to Pseudomonas syringae pv tomato carrying the cognate avrPto gene. Overexpression of Pto under the cauliflower mosaic virus 35S promoter activates spontaneous lesions and confers disease resistance in tomato (Lycopersicon esculentum) plants in the absence of avrPto. Here, we show that these AvrPto-independent defenses require a functional Prf gene. Several Pto-interacting (Pti) proteins are thought to play a role in Pto-mediated defense pathways. To test if interactions with Pti proteins are required for the AvrPto-independent defense responses by Pto overexpression, we isolated several Pto mutants that were unable to interact with one or more Pti proteins, but retained normal interaction with AvrPto. Overexpression of two mutants, PtoG50S and PtoR150S, failed to activate AvrPto-independent defense responses or confer enhanced resistance to the virulent P. s. pv tomato. When introduced into plants carrying 35S::Pto, 35S::PtoG50S dominantly suppressed the AvrPto-independent resistance caused by former transgene. 35S::PtoG50S also blocked the induction of a number of defense genes by the wild-type 35S::Pto. However, 35S::PtoG50S and 35S::PtoR150S plants were completely resistant to P. s. pv tomato (avrPto), indicating a normal gene-for-gene resistance. Furthermore, 35S::PtoG50S plants exhibited normal induction of defense genes in recognition of avrPto. Thus, the AvrPto-independent defense activation and gene-for-gene resistance mediated by Pto are functionally separable.

1 This work was supported by the National Science Foundation (grant no. MCB9808701 to J.-M.Z.) and by the U.S. Department of Agriculture (grant no. 9802511 to X.Y.T.). This is Kansas Agricultural Experimental Station contribution no. 02-170-A.

* Corresponding author; e-mail jzhou{at}ksu.edu; fax 785-532- 5692.

© 2003 American Society of Plant Biologists


This article has been cited by other articles:


Home page
 Plant CellHome page
Y. Luo, K. S. Caldwell, T. Wroblewski, M. E. Wright, and R. W. Michelmore
Proteolysis of a Negative Regulator of Innate Immunity Is Dependent on Resistance Genes in Tomato and Nicotiana benthamiana and Induced by Multiple Bacterial Effectors
PLANT CELL, August 1, 2009; 21(8): 2458 - 2472.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Xiao, P. Giavalisco, and G. B. Martin
Pseudomonas syringae Type III Effector AvrPtoB Is Phosphorylated in Plant Cells on Serine 258, Promoting Its Virulence Activity
J. Biol. Chem., October 19, 2007; 282(42): 30737 - 30744.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. E. Rose, R. W. Michelmore, and C. H. Langley
Natural Variation in the Pto Disease Resistance Gene Within Species of Wild Tomato (Lycopersicon). II. Population Genetics of Pto
Genetics, March 1, 2007; 175(3): 1307 - 1319.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. S. Mucyn, A. Clemente, V. M.E. Andriotis, A. L. Balmuth, G. E.D. Oldroyd, B. J. Staskawicz, and J. P. Rathjen
The Tomato NBARC-LRR Protein Prf Interacts with Pto Kinase in Vivo to Regulate Specific Plant Immunity
PLANT CELL, October 1, 2006; 18(10): 2792 - 2806.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Bernal, Q. Pan, J. Pollack, L. Rose, A. Kozik, N. Willits, Y. Luo, M. Guittet, E. Kochetkova, and R. W. Michelmore
Functional Analysis of the Plant Disease Resistance Gene Pto Using DNA Shuffling
J. Biol. Chem., June 17, 2005; 280(24): 23073 - 23083.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. S. Mysore, M. D. D'Ascenzo, X. He, and G. B. Martin
Overexpression of the Disease Resistance Gene Pto in Tomato Induces Gene Expression Changes Similar to Immune Responses in Human and Fruitfly
Plant Physiology, August 1, 2003; 132(4): 1901 - 1912.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists