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First published online February 27, 2003; 10.1104/pp.102.018598

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Plant Physiol, March 2003, Vol. 131, pp. 1270-1282

Transgenic Studies on the Involvement of Cytokinin and Gibberellin in Male Development

Shihshieh Huang,* R. Eric Cerny, Youlin Qi, Deepti Bhat, Carrie M. Aydt, Doris D. Hanson, Kathleen P. Malloy, and Linda A. Ness

Mystic Research, Monsanto Company, 62 Maritime Drive, Mystic, Connecticut 06355 (S.H., K.P.M.); and Monsanto Company, 700 Chesterfield Parkway North, St. Louis, Missouri 63198 (R.E.C., Y.Q., D.B., C.M.A., D.D.H., L.A.N.)

Numerous plant hormones interact during plant growth and development. Elucidating the role of these various hormones on particular tissue types or developmental stages has been difficult with exogenous applications or constitutive expression studies. Therefore, we used tissue-specific promoters expressing CKX1 and gai, genes involved in oxidative cytokinin degradation and gibberellin (GA) signal transduction, respectively, to study the roles of cytokinin and GA in male organ development. Accumulation of CKX1 in reproductive tissues of transgenic maize (Zea mays) resulted in male-sterile plants. The male development of these plants was restored by applications of kinetin and thidiazuron. Similarly, expression of gai specifically in anthers and pollen of tobacco (Nicotiana tabacum) and Arabidopsis resulted in the abortion of these respective tissues. The gai-induced male-sterile phenotype exhibited by the transgenic plants was reversible by exogenous applications of kinetin. Our results provide molecular evidence of the involvement of cytokinin and GA in male development and support the hypothesis that the male development is controlled in concert by multiple hormones. These studies also suggest a potential method for generating maintainable male sterility in plants by using existing agrochemicals that would reduce the expense of seed production for existing hybrid crops and provide a method to produce hybrid varieties of traditionally non-hybrid crops.

* Corresponding author; e-mail shihshieh.huang{at}na1.monsanto.com; fax 860-572-5240.

© 2003 American Society of Plant Biologists


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