First published online January 30, 2003; 10.1104/pp.016915
Plant Physiol, March 2003, Vol. 131, pp. 1431-1439
One-Carbon Metabolism in Plants. Regulation of Tetrahydrofolate
Synthesis during Germination and Seedling Development
Samuel
Jabrin,
Stéphane
Ravanel,*
Bernadette
Gambonnet,
Roland
Douce, and
Fabrice
Rébeillé
Laboratoire de Physiologie Cellulaire Végétale,
Unité Mixte de Recherche 5019 Centre National de la Recherche
Scientifique-Commissariat à l'Energie Atomique-Université
Joseph Fourier Grenoble I, Département Réponse et Dynamique
Cellulaires, Commissariat à l'Energie Atomique-Grenoble, 17 rue
des Martyrs, 38054 Grenoble Cedex 9, France
Tetrahydrofolate (THF) is a central cofactor for one-carbon
transfer reactions in all living organisms. In this study, we analyzed
the expression of dihydropterin pyrophosphokinase-dihydropteroate synthase (HPPK-DHPS) in pea (Pisum sativum) organs
during development, and so the capacity to synthesize dihydropteroate,
an intermediate in the de novo THF biosynthetic pathway. During
seedling development, all of the examined organs/tissues contain THF
coenzymes, collectively termed folate, and express the HPPK-DHPS
enzyme. This suggests that each organ/tissue is autonomous for the
synthesis of THF. During germination, folate accumulates in cotyledons
and embryos, but high amounts of HPPK-DHPS are only observed in
embryos. During organ differentiation, folate is synthesized
preferentially in highly dividing tissues and in photosynthetic leaves.
This is associated with high levels of the HPPK-DHPS mRNA and protein, and a pool of folate 3- to 5-fold higher than in the rest of the plant.
In germinating embryos and in meristematic tissues, the high capacity
to synthesize and accumulate folate correlates with the general
resumption of cell metabolism and the high requirement for nucleotide
synthesis, major cellular processes involving folate coenzymes. The
particular status of folate synthesis in leaves is related to light.
Thus, when illuminated, etiolated leaves gradually accumulate the
HPPK-DHPS enzyme and folate. This suggests that folate synthesis plays
an important role in the transition from heterotrophic to
photoautotrophic growth. Analysis of the intracellular distribution of
folate in green and etiolated leaves indicates that the coenzymes
accumulate mainly in the cytosol, where they can supply the high demand
for methyl groups.
*
Corresponding author; e-mail sravanel{at}cea.fr; fax
33-4-38-78-50-91.
© 2003 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
J. Moldt, R. Pokorny, C. Orth, U. Linne, Y. Geisselbrecht, M. A. Marahiel, L.-O. Essen, and A. Batschauer
Photoreduction of the Folate Cofactor in Members of the Photolyase Family
J. Biol. Chem.,
August 7, 2009;
284(32):
21670 - 21683.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Waller, T. A. Akhtar, A. Lara-Nunez, J. F. Gregory III, R. P. McQuinn, J. J. Giovannoni, and A. D. Hanson
Developmental and Feedforward Control of the Expression of Folate Biosynthesis Genes in Tomato Fruit
Mol Plant,
August 3, 2009;
(2009)
ssp057v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Loizeau, V. De Brouwer, B. Gambonnet, A. Yu, J.-P. Renou, D. Van Der Straeten, W. E. Lambert, F. Rebeille, and S. Ravanel
A Genome-Wide and Metabolic Analysis Determined the Adaptive Response of Arabidopsis Cells to Folate Depletion Induced by Methotrexate
Plant Physiology,
December 1, 2008;
148(4):
2083 - 2095.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Loizeau, B. Gambonnet, G.-F. Zhang, G. Curien, S. Jabrin, D. Van Der Straeten, W. E. Lambert, F. Rebeille, and S. Ravanel
Regulation of One-Carbon Metabolism in Arabidopsis: The N-Terminal Regulatory Domain of Cystathionine {gamma}-Synthase Is Cleaved in Response to Folate Starvation
Plant Physiology,
October 1, 2007;
145(2):
491 - 503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Storozhenko, O. Navarrete, S. Ravanel, V. De Brouwer, P. Chaerle, G.-F. Zhang, O. Bastien, W. Lambert, F. Rebeille, and D. Van Der Straeten
Cytosolic Hydroxymethyldihydropterin Pyrophosphokinase/Dihydropteroate Synthase from Arabidopsis thaliana: A SPECIFIC ROLE IN EARLY DEVELOPMENT AND STRESS RESPONSE
J. Biol. Chem.,
April 6, 2007;
282(14):
10749 - 10761.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Sun, F. Beck, R. W. Knispel, F. Siedler, B. Scheffer, S. Nickell, W. Baumeister, and I. Nagy
Proteomics Analysis of Thermoplasma acidophilum with a Focus on Protein Complexes
Mol. Cell. Proteomics,
March 1, 2007;
6(3):
492 - 502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. J. Klaus, E. R. S. Kunji, G. G. Bozzo, A. Noiriel, R. D. de la Garza, G. J. C. Basset, S. Ravanel, F. Rebeille, J. F. Gregory III, and A. D. Hanson
Higher Plant Plastids and Cyanobacteria Have Folate Carriers Related to Those of Trypanosomatids
J. Biol. Chem.,
November 18, 2005;
280(46):
38457 - 38463.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bedhomme, M. Hoffmann, E. A. McCarthy, B. Gambonnet, R. G. Moran, F. Rebeille, and S. Ravanel
Folate Metabolism in Plants: AN ARABIDOPSIS HOMOLOG OF THE MAMMALIAN MITOCHONDRIAL FOLATE TRANSPORTER MEDIATES FOLATE IMPORT INTO CHLOROPLASTS
J. Biol. Chem.,
October 14, 2005;
280(41):
34823 - 34831.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. AGARWAL and A. GROVER
Isolation and Transcription Profiling of Low-O2 Stress-Associated cDNA Clones from the Flooding-stress-tolerant FR13A Rice Genotype
Ann. Bot.,
October 1, 2005;
96(5):
831 - 844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Orsomando, R. D. de la Garza, B. J. Green, M. Peng, P. A. Rea, T. J. Ryan, J. F. Gregory III, and A. D. Hanson
Plant {gamma}-Glutamyl Hydrolases and Folate Polyglutamates: CHARACTERIZATION, COMPARTMENTATION, AND CO-OCCURRENCE IN VACUOLES
J. Biol. Chem.,
August 12, 2005;
280(32):
28877 - 28884.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Goyer, E. Collakova, R. D. de la Garza, E. P. Quinlivan, J. Williamson, J. F. Gregory III, Y. Shachar-Hill, and A. D. Hanson
5-Formyltetrahydrofolate Is an Inhibitory but Well Tolerated Metabolite in Arabidopsis Leaves
J. Biol. Chem.,
July 15, 2005;
280(28):
26137 - 26142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ravanel, M. A. Block, P. Rippert, S. Jabrin, G. Curien, F. Rebeille, and R. Douce
Methionine Metabolism in Plants: CHLOROPLASTS ARE AUTONOMOUS FOR DE NOVO METHIONINE SYNTHESIS AND CAN IMPORT S-ADENOSYLMETHIONINE FROM THE CYTOSOL
J. Biol. Chem.,
May 21, 2004;
279(21):
22548 - 22557.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Rajjou, K. Gallardo, I. Debeaujon, J. Vandekerckhove, C. Job, and D. Job
The Effect of {alpha}-Amanitin on the Arabidopsis Seed Proteome Highlights the Distinct Roles of Stored and Neosynthesized mRNAs during Germination
Plant Physiology,
April 1, 2004;
134(4):
1598 - 1613.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
