First published online February 6, 2003; 10.1104/pp.015628
Plant Physiol, March 2003, Vol. 131, pp. 1450-1459
CsAGP1, a Gibberellin-Responsive Gene from Cucumber
Hypocotyls, Encodes a Classical Arabinogalactan Protein and Is Involved
in Stem Elongation
Me Hea
Park,
Yoshihito
Suzuki,*
Makiko
Chono,
J. Paul
Knox, and
Isomaro
Yamaguchi
Department of Applied Biological Chemistry, The University of
Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan (M.H.P., Y.S., I.Y.); National
Agricultural Research Organization, National Institute of Crop Science,
Department of Wheat and Barley, 2-1-18 Kannondai, Tsukuba 305-8518,
Japan (M.C.); and Centre for Plant Sciences, University of Leeds, Leeds
LS2 9JT, United Kingdom (J.P.K.)
Fluorescence differential display was used to isolate the
gibberellin (GA)-responsive gene, CsAGP1, from cucumber
(Cucumis sativus) hypocotyls. A sequence analysis of
CsAGP1 indicated that the gene putatively encodes a
"classical" arabinogalactan protein (AGP) in cucumber. Transgenic
tobacco (Nicotiana tabacum) plants overexpressing
CsAGP1 under the control of the cauliflower mosaic virus
35S promoter produced a Y( Glc)3-reactive proteoglycan in addition to AGPs present in wild-type tobacco plants. Immuno-dot blotting of the product, using anti-AGP antibodies, showed that the
CsAGP1 protein had the AGP epitopes common to AGP families. The
transcription level of CsAGP1 in cucumber hypocotyls
increased in response not only to GA but also to indole-3-acetic acid.
Although CsAGP1 is expressed in most vegetative tissues
of cucumber, including the shoot apices and roots, the GA treatment
resulted in an increase in the mRNA level of CsAGP1 only
in the upper part of the hypocotyls. Y(Glc)3, which
selectively binds AGPs, inhibited the hormone-promoted elongation of
cucumber seedling hypocotyls. Transgenic plants ectopically expressing
CsAGP1 showed a taller stature and earlier flowering
than the wild-type plants. These observations suggest that
CsAGP1 is involved in stem elongation.
*
Corresponding author; e-mail
ayoshi{at}pgr1.ch.a.u-tokyo.ac.jp; fax 81-3-5841-8025.
© 2003 American Society of Plant Biologists
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