First published online January 23, 2003; 10.1104/pp.009902
Plant Physiol, March 2003, Vol. 131, pp. 985-997
Expression of an Expansin Gene Is Correlated with Root Elongation
in Soybean1,[w]
Dong-Keun
Lee,2
Ji Hoon
Ahn,23
Sang-Kee
Song,4
Yang Do
Choi, and
Jong Seob
Lee*
School of Biological Sciences, Seoul National University, Seoul
151-742, Korea (D.-K.L., J.H.A., S.-K.S., J.S.L.); and School of
Agricultural Biotechnology and Crop Functional Genomics Center, Seoul
National University, Suwon 441-744, Korea (Y.D.C.)
Expansin is a family of proteins that catalyze long-term expansion
of cell walls and has been considered a principal protein that affects
cell expansion in plants. We have identified the first root-specific
expansin gene in soybean (Glycine max),
GmEXP1, which may be responsible for root elongation.
Expression levels of GmEXP1 were very high in the roots
of 1- to 5-d-old seedlings, in which rapid root elongation takes place.
Furthermore, GmEXP1 mRNA was most abundant in the root
tip region, where cell elongation occurs, but scarce in the region of
maturation, where cell elongation ceases, implying that its expression
is closely related to root development processes. In situ hybridization
showed that GmEXP1 transcripts were preferentially
present in the epidermal cells and underlying cell layers in the root
tip of the primary and secondary roots. Ectopic expression of
GmEXP1 accelerated the root growth of transgenic tobacco
(Nicotiana tabacum) seedlings, and the roots showed
insensitivity to obstacle-touching stress. These results imply that the
GmEXP1 gene plays an important role in root development
in soybean, especially in the elongation and/or initiation of the
primary and secondary roots.
1
This work was supported by the Korea Science and
Engineering Foundation through the Plant Metabolism Research Center of
Kyung Hee University and by the Crop Functional Genomics Center of the 21C Frontier Program of the Ministry of Science and Technology of Korea
(grant to J.S.L.).
2
These authors contributed equally to the paper.
3
Present address: Graduate School of Biotechnology, Korea
University, Seoul 136-701, Korea.
4
Present address: Department of Biology, University of
Michigan, Ann Arbor, MI, 48109-1048.
[w]
The online version of this article contains Web-only
data. The supplemental material is available at
www.plantphysiol.org.
*
Corresponding author; e-mail jongslee{at}plaza.snu.ac.kr; fax
82-2-872-1993.
© 2003 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
D.-K. Lee, M. Geisler, and P. S. Springer
LATERAL ORGAN FUSION1 and LATERAL ORGAN FUSION2 function in lateral organ separation and axillary meristem formation in Arabidopsis
Development,
July 15, 2009;
136(14):
2423 - 2432.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sasidharan, C.C. Chinnappa, L. A.C.J. Voesenek, and R. Pierik
The Regulation of Cell Wall Extensibility during Shade Avoidance: A Study Using Two Contrasting Ecotypes of Stellaria longipes
Plant Physiology,
November 1, 2008;
148(3):
1557 - 1569.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Carey and D. J. Cosgrove
Portrait of the Expansin Superfamily in Physcomitrella patens: Comparisons with Angiosperm Expansins
Ann. Bot.,
June 1, 2007;
99(6):
1131 - 1141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Muller, G. Bourdais, B. Reidy, C. Bencivenni, A. Massonneau, P. Condamine, G. Rolland, G. Conejero, P. Rogowsky, and F. Tardieu
Association of Specific Expansins with Growth in Maize Leaves Is Maintained under Environmental, Genetic, and Developmental Sources of Variation
Plant Physiology,
January 1, 2007;
143(1):
278 - 290.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kwasniewski and I. Szarejko
Molecular Cloning and Characterization of beta-Expansin Gene Related to Root Hair Formation in Barley
Plant Physiology,
July 1, 2006;
141(3):
1149 - 1158.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Delis, M. Dimou, E. Flemetakis, G. Aivalakis, and P. Katinakis
A root- and hypocotyl-specific gene coding for copper-containing amine oxidase is related to cell expansion in soybean seedlings
J. Exp. Bot.,
January 1, 2006;
57(1):
101 - 111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
