First published online January 23, 2003; 10.1104/pp.014712
Plant Physiol, April 2003, Vol. 131, pp. 1511-1517
Phloem-Localizing Sulfate Transporter, Sultr1;3, Mediates
Re-Distribution of Sulfur from Source to Sink Organs in
Arabidopsis1
Naoko
Yoshimoto,
Eri
Inoue,
Kazuki
Saito,
Tomoyuki
Yamaya, and
Hideki
Takahashi*
RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama, 230-0045, Japan (N.Y., E.I., T.Y., H.T.); Chiba University,
Graduate School of Pharmaceutical Sciences, 1-33 Yayoi-cho, Inage-ku,
Chiba, 263-8522, Japan (N.Y., K.S.); Tohoku University, Graduate
School of Agricultural Sciences, 1-1 Tsutsumidori-Amamiyamachi,
Aoba-ku, Sendai, 981-8555, Japan (T.Y.)
For the effective recycling of nutrients, vascular plants transport
pooled inorganic ions and metabolites through the sieve tube. A novel
sulfate transporter gene, Sultr1;3, was
identified as an essential member contributing to this process for
redistribution of sulfur source in Arabidopsis.
Sultr1;3 belonged to the family of
high-affinity sulfate transporters, and was able to complement the
yeast sulfate transporter mutant. The fusion protein of Sultr1;3 and
green fluorescent protein was expressed by the
Sultr1;3 promoter in transgenic plants,
which revealed phloem-specific expression of
Sultr1;3 in Arabidopsis. Sultr1;3-green
fluorescent protein was found in the sieve element-companion cell
complexes of the phloem in cotyledons and roots. Limitation of external
sulfate caused accumulation of Sultr1;3
mRNA both in leaves and roots. Movement of 35S-labeled
sulfate from cotyledons to the sink organs was restricted in the T-DNA
insertion mutant of Sultr1;3. These
results provide evidence that Sultr1;3 transporter plays an important
role in loading of sulfate to the sieve tube, initiating the
source-to-sink translocation of sulfur nutrient in Arabidopsis.
1
This work was supported by the Ministry of
Education, Culture, Sports, Science and Technology of Japan, by the
Japan Society for the Promotion of Science, and by Core Research for
Evolutional Science and Technology of Japan Science and Technology.
*
Corresponding author; e-mail hideki{at}postman.riken.go.jp; fax
81-45-503-9609.
© 2003 American Society of Plant Biologists
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