First published online March 20, 2003; 10.1104/pp.102.016725
Plant Physiol, April 2003, Vol. 131, pp. 1566-1575
Cloning, Expression, and Characterization of Sorbitol
Transporters from Developing Sour Cherry Fruit and Leaf Sink
Tissues1
Zhifang
Gao,
Laurence
Maurousset,
Remi
Lemoine,
Sang-Dong
Yoo,
Steven
van Nocker, and
Wayne
Loescher*
Department of Horticulture, Michigan State University, East
Lansing, Michigan 48824 (Z.G., S.-D.Y., S.v.N., W.L.); and Laboratoire
de Physiologie et Biochimie Végétales, Unité Mixte de
Recherches 6161, Centre National de la Recherche Scientifique,
Bâtiment Botanique, Université de Poitiers, 40 Avenue du
Recteur Pineau, 86022 Poitiers cedex, France (L.M., R.L.)
The acyclic polyol sorbitol is a primary photosynthetic
product and the principal photosynthetic transport substance in many economically important members of the family Rosaceace (e.g. almond [Prunus dulcis (P. Mill.) D.A. Webber], apple
[Malus pumila P. Mill.], cherry
[Prunus spp.], peach [Prunus persica
L. Batsch], and pear [Pyrus communis]). To understand
key steps in long-distance transport and particularly partitioning and
accumulation of sorbitol in sink tissues, we have cloned two sorbitol
transporter genes (PcSOT1 and
PcSOT2) from sour cherry (Prunus cerasus)
fruit tissues that accumulate large quantities of sorbitol. Sorbitol
uptake activities and other characteristics were measured by
heterologous expression of PcSOT1 and
PcSOT2 in yeast (Saccharomyces
cerevisiae). Both genes encode proton-dependent,
sorbitol-specific transporters with similar affinities
(Km sorbitol of 0.81 mM for
PcSOT1 and 0.64 mM for PcSOT2). Analyses of gene expression
of these transporters, however, suggest different roles during leaf and
fruit development. PcSOT1 is expressed throughout fruit
development, but especially when growth and sorbitol accumulation rates
are highest. In leaves, PcSOT1 expression is highest in
young, expanding tissues, but substantially less in mature leaves. In
contrast, PcSOT2 is mainly expressed only early in fruit
development and not in leaves. Compositional analyses suggest that
transport mediated by PcSOT1 and PcSOT2 plays a major role in sorbitol
and dry matter accumulation in sour cherry fruits. Presence of these
transporters and the high fruit sorbitol concentrations suggest that
there is an apoplastic step during phloem unloading and accumulation in
these sink tissues. Expression of PcSOT1 in young leaves
before completion of the transition from sink to source is further
evidence for a role in determining sink activity.
1
This work was supported by Michigan State
University (Project GREEEN) and by the Centre National de la Recherche
Scientifique (France; special fellowship to Z.G.).
*
Corresponding author; e-mail loescher{at}msu.edu; fax
517-432-3490.
© 2003 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
N. Garcia-Marino, F. de la Torre, and A.J. Matilla
Organic Acids and Soluble Sugars in Edible and Nonedible Parts of Damson Plum (Prunus domestica L. subsp. insititia cv. Syriaca) Fruits During Development and Ripening
Food Science and Technology International,
April 1, 2008;
14(2):
187 - 193.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. Schneider, D. Beyhl, R. Hedrich, and N. Sauer
Functional and Physiological Characterization of Arabidopsis INOSITOL TRANSPORTER1, a Novel Tonoplast-Localized Transporter for myo-Inositol
PLANT CELL,
April 1, 2008;
20(4):
1073 - 1087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Juchaux-Cachau, L. Landouar-Arsivaud, J.-P. Pichaut, C. Campion, B. Porcheron, J. Jeauffre, N. Noiraud-Romy, P. Simoneau, L. Maurousset, and R. Lemoine
Characterization of AgMaT2, a Plasma Membrane Mannitol Transporter from Celery, Expressed in Phloem Cells, Including Phloem Parenchyma Cells
Plant Physiology,
September 1, 2007;
145(1):
62 - 74.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Pommerrenig, F. S. Papini-Terzi, and N. Sauer
Differential Regulation of Sorbitol and Sucrose Loading into the Phloem of Plantago major in Response to Salt Stress
Plant Physiology,
June 1, 2007;
144(2):
1029 - 1038.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Conde, P. Silva, A. Agasse, R. Lemoine, S. Delrot, R. Tavares, and H. Geros
Utilization and Transport of Mannitol in Olea europaea and Implications for Salt Stress Tolerance
Plant Cell Physiol.,
January 1, 2007;
48(1):
42 - 53.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Teo, Y. Suzuki, S. L. Uratsu, B. Lampinen, N. Ormonde, W. K. Hu, T. M. DeJong, and A. M. Dandekar
Colloquium Paper: Silencing leaf sorbitol synthesis alters long-distance partitioning and apple fruit quality
PNAS,
December 5, 2006;
103(49):
18842 - 18847.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hemmerlin, D. Tritsch, M. Hartmann, K. Pacaud, J.-F. Hoeffler, A. van Dorsselaer, M. Rohmer, and T. J. Bach
A Cytosolic Arabidopsis D-Xylulose Kinase Catalyzes the Phosphorylation of 1-Deoxy-D-Xylulose into a Precursor of the Plastidial Isoprenoid Pathway
Plant Physiology,
October 1, 2006;
142(2):
441 - 457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Conde, A. Agasse, D. Glissant, R. Tavares, H. Geros, and S. Delrot
Pathways of Glucose Regulation of Monosaccharide Transport in Grape Cells
Plant Physiology,
August 1, 2006;
141(4):
1563 - 1577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Schneider, A. Schneidereit, K. R. Konrad, M.-R. Hajirezaei, M. Gramann, R. Hedrich, and N. Sauer
Arabidopsis INOSITOL TRANSPORTER4 Mediates High-Affinity H+ Symport of Myoinositol across the Plasma Membrane
Plant Physiology,
June 1, 2006;
141(2):
565 - 577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Vignault, M. Vachaud, B. Cakir, D. Glissant, F. Dedaldechamp, M. Buttner, R. Atanassova, P. Fleurat-Lessard, R. Lemoine, and S. Delrot
VvHT1 encodes a monosaccharide transporter expressed in the conducting complex of the grape berry phloem
J. Exp. Bot.,
May 1, 2005;
56(415):
1409 - 1418.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ferreira, F. van Voorst, A. Martins, L. Neves, R. Oliveira, M. C. Kielland-Brandt, C. Lucas, and A. Brandt
A Member of the Sugar Transporter Family, Stl1p Is the Glycerol/H+ Symporter in Saccharomyces cerevisiae
Mol. Biol. Cell,
April 1, 2005;
16(4):
2068 - 2076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Reinders, J. A. Panshyshyn, and J. M. Ward
Analysis of Transport Activity of Arabidopsis Sugar Alcohol Permease Homolog AtPLT5
J. Biol. Chem.,
January 14, 2005;
280(2):
1594 - 1602.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-S. Klepek, D. Geiger, R. Stadler, F. Klebl, L. Landouar-Arsivaud, R. Lemoine, R. Hedrich, and N. Sauer
Arabidopsis POLYOL TRANSPORTER5, a New Member of the Monosaccharide Transporter-Like Superfamily, Mediates H+-Symport of Numerous Substrates, Including myo-Inositol, Glycerol, and Ribose
PLANT CELL,
January 1, 2005;
17(1):
204 - 218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Watari, Y. Kobae, S. Yamaki, K. Yamada, K. Toyofuku, T. Tabuchi, and K. Shiratake
Identification of Sorbitol Transporters Expressed in the Phloem of Apple Source Leaves
Plant Cell Physiol.,
August 15, 2004;
45(8):
1032 - 1041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-Y. Zhang, Y.-B. Peng, S. Pelleschi-Travier, Y. Fan, Y.-F. Lu, Y.-M. Lu, X.-P. Gao, Y.-Y. Shen, S. Delrot, and D.-P. Zhang
Evidence for Apoplasmic Phloem Unloading in Developing Apple Fruit
Plant Physiology,
May 1, 2004;
135(1):
574 - 586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ramsperger-Gleixner, D. Geiger, R. Hedrich, and N. Sauer
Differential Expression of Sucrose Transporter and Polyol Transporter Genes during Maturation of Common Plantain Companion Cells
Plant Physiology,
January 1, 2004;
134(1):
147 - 160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
