First published online March 6, 2003; 10.1104/pp.102.016444
Plant Physiol, April 2003, Vol. 131, pp. 1602-1612
Distribution of Fucose-Containing Xyloglucans in Cell Walls of
the mur1 Mutant of Arabidopsis1
Glenn
Freshour,
Christopher
P.
Bonin,2
Wolf-Dieter
Reiter,
Peter
Albersheim,
Alan G.
Darvill, and
Michael G.
Hahn*
The University of Georgia, Complex Carbohydrate Research Center
(G.F., P.A., A.G.D., M.G.H.) and Departments of Plant Biology (M.G.H.)
and Biochemistry and Molecular Biology (P.A., A.G.D.), 220 Riverbend
Road, Athens, Georgia 30602-4712; and University of Connecticut,
Department of Molecular and Cell Biology, Storrs, Connecticut 06269 (C.P.B., W.-D.R.)
The monoclonal antibody, CCRC-M1, which recognizes a fucose
(Fuc)-containing epitope found principally in the cell wall
polysaccharide xyloglucan, was used to determine the distribution of
this epitope throughout the mur1 mutant of
Arabidopsis. Immunofluorescent labeling of whole seedlings
revealed that mur1 root hairs are stained heavily by
CCRC-M1, whereas the body of the root remains unstained or only lightly
stained. Immunogold labeling showed that CCRC-M1 labeling within the
mur1 root is specific to particular cell walls and cell
types. CCRC-M1 labels all cell walls at the apex of primary roots
2 d and older and the apices of mature lateral roots, but does not
bind to cell walls in lateral root initials. Labeling with CCRC-M1
decreases in mur1 root cells that are undergoing rapid
elongation growth such that, in the mature portions of primary and
lateral roots, only the walls of pericycle cells and the outer walls of
epidermal cells are labeled. Growth of the mutant on Fuc-containing
media restores wild-type labeling, where all cell walls are labeled by
the CCRC-M1 antibody. No labeling was observed in mur1
hypocotyls, shoots, or leaves; stipules are labeled. CCRC-M1 does label
pollen grains within anthers and pollen tube walls. These results
suggest the Fuc destined for incorporation into xyloglucan is
synthesized using one or the other or both isoforms of
GDP-D-mannose 4,6-dehydratase, depending on the cell type
and/or developmental state of the cell.
1
This work was supported by the U.S.
Department of Energy (grant nos. DE-FG02-96ER20220 and
DE-FG02-95ER20203) and in part by the U.S. Department of
Energy-funded Center for Plant and Microbial Complex
Carbohydrates (grant no. DE-FG05-93ER20097).
2
Present address: Department of Biochemistry and
Molecular Biophysics, Columbia University, New York, NY 10032.
*
Corresponding author; e-mail hahn{at}ccrc.uga.edu; fax
706-542-4412.
© 2003 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
D. S. Domozych, I. Sorensen, and W. G. T. Willats
The distribution of cell wall polymers during antheridium development and spermatogenesis in the Charophycean green alga, Chara corallina
Ann. Bot.,
November 1, 2009;
104(6):
1045 - 1056.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Gu, C. J. Wages, K. E. Davis, P. J. Guyett, and M. Bar-Peled
Enzymatic Characterization and Comparison of Various Poaceae UDP-GlcA 4-Epimerase Isoforms
J. Biochem.,
October 1, 2009;
146(4):
527 - 534.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Obel, V. Erben, T. Schwarz, S. Kuhnel, A. Fodor, and M. Pauly
Microanalysis of Plant Cell Wall Polysaccharides
Mol Plant,
September 1, 2009;
2(5):
922 - 932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Q. Phan, S.-J. Kim, and D. C. Bassham
Overexpression of Arabidopsis Sorting Nexin AtSNX2b Inhibits Endocytic Trafficking to the Vacuole
Mol Plant,
November 1, 2008;
1(6):
961 - 976.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. H. Liepman, C. J. Nairn, W. G.T. Willats, I. Sorensen, A. W. Roberts, and K. Keegstra
Functional Genomic Analysis Supports Conservation of Function Among Cellulose Synthase-Like A Gene Family Members and Suggests Diverse Roles of Mannans in Plants
Plant Physiology,
April 1, 2007;
143(4):
1881 - 1893.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Persson, K. H. Caffall, G. Freshour, M. T. Hilley, S. Bauer, P. Poindexter, M. G. Hahn, D. Mohnen, and C. Somerville
The Arabidopsis irregular xylem8 Mutant Is Deficient in Glucuronoxylan and Homogalacturonan, Which Are Essential for Secondary Cell Wall Integrity
PLANT CELL,
January 1, 2007;
19(1):
237 - 255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. Gunnarsson, Q. Zhou, C. Montanier, E. N. Karlsson, H. Brumer III, and M. Ohlin
Engineered xyloglucan specificity in a carbohydrate-binding module
Glycobiology,
December 1, 2006;
16(12):
1171 - 1180.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Coyne, B. Reinap, M. M. Lee, and L. E. Comstock
Human Symbionts Use a Host-Like Pathway for Surface Fucosylation
Science,
March 18, 2005;
307(5716):
1778 - 1781.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Somerville, S. Bauer, G. Brininstool, M. Facette, T. Hamann, J. Milne, E. Osborne, A. Paredez, S. Persson, T. Raab, et al.
Toward a Systems Approach to Understanding Plant Cell Walls
Science,
December 24, 2004;
306(5705):
2206 - 2211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. Bonin, G. Freshour, M. G. Hahn, G. F. Vanzin, and W.-D. Reiter
The GMD1 and GMD2 Genes of Arabidopsis Encode Isoforms of GDP-D-Mannose 4,6-Dehydratase with Cell Type-Specific Expression Patterns
Plant Physiology,
June 1, 2003;
132(2):
883 - 892.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
