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Plant Physiol, April 2003, Vol. 131, pp. 1623-1627
A Reevaluation of the Role of the Heterotrimeric G Protein in
Coupling Light Responses in Arabidopsis1
Alan M.
Jones,*
Joseph R.
Ecker, and
Jin-Gui
Chen
Department of Biology, University of North Carolina, Chapel Hill,
North Carolina 27599 (A.M.J., J.-G.C.); and The Salk Institute for
Biological Studies, La Jolla, California 92037 (J.R.E.)
Previous studies implicated the involvement of a heterotrimeric G
protein in red (R) and far-red (FR) light signal transduction, but
these studies utilized pharmacological or gain-of-function approaches
and, therefore, are indirect tests. Here, we reexamine the role of the
single canonical heterotrimeric G protein in R and FR control of
hypocotyl growth using a loss-of-function approach. Single- and
double-null mutants for the GPA1, AGB1
genes encoding the alpha and beta subunit of the heterotrimeric G
protein, respectively, have wild-type sensitivity to R and FR. Ectopic
overexpression of wild type and a constitutive active form of the alpha
subunit and of the wild-type beta subunit had no effect that can be
unequivocally attributed to altered R and FR responsiveness. These
results preclude a direct role for the heterotrimeric G complex in R
and FR transduction in Arabidopsis leading to growth control in the hypocotyl.
1
This work was supported by the National
Institutes of Health (grant no. GM65989-01 to A.M.J.) and by the
National Science Foundation (grant no. MCB-0209711 to A.M.J.).
*
Corresponding author; e-mail alan_jones{at}unc.edu; fax
919-962-6932.
© 2003 American Society of Plant Biologists
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