Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online March 6, 2003; 10.1104/pp.102.014308

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
131/4/1638    most recent
pp.102.014308v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Steinebrunner, I.
Right arrow Articles by Roux, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steinebrunner, I.
Right arrow Articles by Roux, S. J.
Agricola
Right arrow Articles by Steinebrunner, I.
Right arrow Articles by Roux, S. J.

Plant Physiol, April 2003, Vol. 131, pp. 1638-1647

Disruption of Apyrases Inhibits Pollen Germination in Arabidopsis1

Iris Steinebrunner, Jian Wu,2 Yu Sun,2 Ashley Corbett, and Stanley J. Roux*

Department of Molecular Cell and Developmental Biology, University of Texas, 205 West 24th Street, Austin, Texas 78712-6700

In Arabidopsis, we previously identified two highly similar apyrases, AtAPY1 and AtAPY2. Here, T-DNA knockout (KO) mutations of each gene were isolated in a reverse genetic approach. The single KO mutants lacked a discernible phenotype. The double KO mutants, however, exhibited a complete inhibition of pollen germination, and this correlated with positive beta -glucuronidase staining in the pollen of apyrase promoter:beta -glucuronidase fusion transgenic lines. The vast majority of the pollen grains of these mutants were identical to wild type in size, shape, and nuclear state and were viable as assayed by metabolic activity and plasma membrane integrity. Complementation with either AtAPY1 or AtAPY2 cDNA rescued pollen germination, confirming that the phenotype was apyrase specific. Despite the redundancy of the two apyrases in rescue potential, transmission analyses suggested a greater role for AtAPY2 in male gamete success. The effect of mutant apyrase on the transmission through the female gametophyte was only marginal, and embryo development appeared normal in the absence of apyrases. The male-specific double KO mutation is fully penetrant and shows that apyrases play a crucial role in pollen germination.

1 This work was supported by the National Science Foundation (grant no. IBN-0080363) and by The Texas Higher Education Coordinating Board.

2 These authors contributed equally to this work.

* Corresponding author; e-mail sroux{at}uts.cc.utexas.edu; fax 512-232-3402.

© 2003 American Society of Plant Biologists


This article has been cited by other articles:


Home page
 J Exp BotHome page
S. A. Reichler, J. Torres, A. L. Rivera, V. A. Cintolesi, G. Clark, and S. J. Roux
Intersection of two signalling pathways: extracellular nucleotides regulate pollen germination and pollen tube growth via nitric oxide
J. Exp. Bot., May 1, 2009; 60(7): 2129 - 2138.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. C. Boavida, B. Shuai, H.-J. Yu, G. C. Pagnussat, V. Sundaresan, and S. McCormick
A Collection of Ds Insertional Mutants Associated With Defects in Male Gametophyte Development and Function in Arabidopsis thaliana
Genetics, April 1, 2009; 181(4): 1369 - 1385.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Govindarajulu, S.-Y. Kim, M. Libault, R. H. Berg, K. Tanaka, G. Stacey, and C. G. Taylor
GS52 Ecto-Apyrase Plays a Critical Role during Soybean Nodulation
Plant Physiology, February 1, 2009; 149(2): 994 - 1004.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Riewe, L. Grosman, A. R. Fernie, C. Wucke, and P. Geigenberger
The Potato-Specific Apyrase Is Apoplastically Localized and Has Influence on Gene Expression, Growth, and Development
Plant Physiology, July 1, 2008; 147(3): 1092 - 1109.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. P. Foresi, A. M. Laxalt, C. V. Tonon, C. A. Casalongue, and L. Lamattina
Extracellular ATP Induces Nitric Oxide Production in Tomato Cell Suspensions
Plant Physiology, November 1, 2007; 145(3): 589 - 592.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Wu, I. Steinebrunner, Y. Sun, T. Butterfield, J. Torres, D. Arnold, A. Gonzalez, F. Jacob, S. Reichler, and S. J. Roux
Apyrases (Nucleoside Triphosphate-Diphosphohydrolases) Play a Key Role in Growth Control in Arabidopsis
Plant Physiology, June 1, 2007; 144(2): 961 - 975.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. U. Igamberdiev and L. A. Kleczkowski
Equilibration of adenylates in the mitochondrial intermembrane space maintains respiration and regulates cytosolic metabolism
J. Exp. Bot., July 1, 2006; 57(10): 2133 - 2141.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. J. Song, I. Steinebrunner, X. Wang, S. C. Stout, and S. J. Roux
Extracellular ATP Induces the Accumulation of Superoxide via NADPH Oxidases in Arabidopsis
Plant Physiology, April 1, 2006; 140(4): 1222 - 1232.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Gass, T. Glagotskaia, S. Mellema, J. Stuurman, M. Barone, T. Mandel, U. Roessner-Tunali, and C. Kuhlemeier
Pyruvate Decarboxylase Provides Growing Pollen Tubes with a Competitive Advantage in Petunia
PLANT CELL, August 1, 2005; 17(8): 2355 - 2368.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. A. Cole, L. Synek, V. Zarsky, and J. E. Fowler
SEC8, a Subunit of the Putative Arabidopsis Exocyst Complex, Facilitates Pollen Germination and Competitive Pollen Tube Growth
Plant Physiology, August 1, 2005; 138(4): 2005 - 2018.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. B. McAlvin and G. Stacey
Transgenic Expression of the Soybean Apyrase in Lotus japonicus Enhances Nodulation
Plant Physiology, April 1, 2005; 137(4): 1456 - 1462.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. A. Johnson, K. von Besser, Q. Zhou, E. Smith, G. Aux, D. Patton, J. Z. Levin, and D. Preuss
Arabidopsis hapless Mutations Define Essential Gametophytic Functions
Genetics, October 1, 2004; 168(2): 971 - 982.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. R. Jeter, W. Tang, E. Henaff, T. Butterfield, and S. J. Roux
Evidence of a Novel Cell Signaling Role for Extracellular Adenosine Triphosphates and Diphosphates in Arabidopsis
PLANT CELL, October 1, 2004; 16(10): 2652 - 2664.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. Lalanne, C. Michaelidis, J. M. Moore, W. Gagliano, A. Johnson, R. Patel, R. Howden, J.-P. Vielle-Calzada, U. Grossniklaus, and D. Twell
Analysis of Transposon Insertion Mutants Highlights the Diversity of Mechanisms Underlying Male Progamic Development in Arabidopsis
Genetics, August 1, 2004; 167(4): 1975 - 1986.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. McCormick
Control of Male Gametophyte Development
PLANT CELL, June 1, 2004; 16(suppl_1): S142 - S153.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. R. Hicks, E. Rojo, S. Hong, D. G. Carter, and N. V. Raikhel
Geminating Pollen Has Tubular Vacuoles, Displays Highly Dynamic Vacuole Biogenesis, and Requires VACUOLESS1 for Proper Function
Plant Physiology, March 1, 2004; 134(3): 1227 - 1239.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists