First published online March 20, 2003; 10.1104/pp.014035
Plant Physiol, April 2003, Vol. 131, pp. 1705-1717
Ethylene Regulates Monomeric GTP-Binding Protein Gene Expression
and Activity in Arabidopsis1
Igor E.
Moshkov,2
Luis A.J.
Mur,2
Galina V.
Novikova,2
Aileen R.
Smith, and
Michael A.
Hall*
Timiryazev Institute of Plant Physiology Russian Academy of
Sciences, Botanicheskaya 35, Moscow 127276, Russia (I.E.M.,
G.V.N.); and Institute of Biological Sciences, University of Wales,
Aberystwyth SY23 3DA, United Kingdom (L.A.J.M., A.R.S., M.A.H.)
Ethylene rapidly and transiently up-regulates the activity of
several monomeric GTP-binding proteins (monomeric G proteins) in leaves
of Arabidopsis as determined by two-dimensional gel electrophoresis and
autoradiographic analyses. The activation is suppressed by the
receptor-directed inhibitor 1-methylcyclopropene. In the
etr1-1 mutant, constitutive activity of all the
monomeric G proteins activated by ethylene is down-regulated relative
to wild type, and ethylene treatment has no effect on the levels of
activity. Conversely, in the ctr1-1 mutant, several of
the monomeric G proteins activated by ethylene are constitutively up-regulated. However, the activation profile of ctr1-1
does not exactly mimic that of ethylene-treated wild type. Biochemical and molecular evidence suggested that some of these monomeric G
proteins are of the Rab class. Expression of the genes for a number of
monomeric G proteins in response to ethylene was investigated by
reverse transcriptase-PCR. Rab8 and
Ara3 expression was increased within 10 min of ethylene
treatment, although levels fell back significantly by 40 min. In the
etr1-1 mutant, expression of Rab8 was
lower than wild type and unaffected by ethylene; in
ctr1-1, expression of Rab8 was much
higher than wild type and comparable with that seen in ethylene
treatments. Expression in ctr1-1 was also unaffected by
ethylene. Thus, the data indicate a role for monomeric G proteins in
ethylene signal transduction.
1
This work was supported in part by INTAS
(grant no. 99-01200).
2
These authors contributed equally to the paper.
*
Corresponding author; e-mail mzh{at}aber.ac.uk; fax
44-1970-622307.
© 2003 American Society of Plant Biologists
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