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Plant Physiol, April 2003, Vol. 131, pp. 1834-1842
The Gluconeogenic Enzyme Phosphoenolpyruvate
Carboxykinase in Arabidopsis Is Essential for Seedling
Establishment
Elizabeth L.
Rylott,
Alison
D.
Gilday, and
Ian A.
Graham*
Centre for Novel Agricultural Products, Department of Biology (Area
7), University of York, P.O. Box 373, York YO10 5YW, United
Kingdom
Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes
the conversion of oxaloacetate to phosphoenolpyruvate in
the gluconeogenic production of sugars from storage oil in germinating
oilseeds. Here, we present the results of analysis on PEPCK antisense
Arabidopsis plants with a range of enzyme activities from 20% to 80%
of wild-type levels. There is a direct correlation between enzyme
activity and seedling establishment during early post-germinative
growth, thus demonstrating the absolute requirement of PEPCK and
gluconeogenesis in this process. Soluble sugar levels in the
35S-PCK1 antisense seedlings are reduced and seedling
establishment can be rescued with an exogenous supply of sucrose. We
observed an increase in the respiration of acetyl coenzyme A units
released from fatty acid  -oxidation and a corresponding decrease in
the production of sugars with decreasing enzyme activity in 2-d-old
antisense seedlings. The 35S-PCK1 antisense lines have a
more extreme phenotype when compared with Arabidopsis mutants disrupted
in the glyoxylate cycle. We conclude that the 35S-PCK1
antisense seedlings are compromised in the ability to use both storage
lipid and storage protein through gluconeogenesis to produce soluble sugars.
*
Corresponding author; e-mail iag1{at}york.ac.uk; fax
44-01904-328762.
© 2003 American Society of Plant Biologists
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