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Plant Physiol, May 2003, Vol. 132, pp. 106-117
Overexpression of a Knotted-Like Homeobox Gene of
Potato Alters Vegetative Development by Decreasing Gibberellin
Accumulation1
Faye M.
Rosin,2
Jennifer K.
Hart,2
Harry T.
Horner,
Peter J.
Davies, and
David J.
Hannapel*
Interdepartmental Program in Molecular, Cellular, and Developmental
Biology (F.M.R., D.J.H.), Interdepartmental Genetics Major (J.K.H.,
D.J.H.), Department of Horticulture (F.M.R., J.K.H., D.J.H.), and
Department of Botany, Bessey Microscopy Facility (H.T.H.), Iowa State
University, Ames, Iowa 50011; and Department of Plant Biology, Cornell
University, Ithaca, New York 14853 (P.J.D.)
Potato (Solanum tuberosum) homeobox 1 (POTH1) is a class I homeobox gene isolated from an
early-stage tuber cDNA library. The RNA expression pattern of
POTH1, unlike that of most other class I
knotted-like homeobox genes, is widespread in the cells of both indeterminate and differentiated tissues. Using in
situ hybridization, POTH1 transcripts were detected in
meristematic cells, leaf primordia, and the vascular procambium of the
young stem. Overexpression of POTH1 produced dwarf
plants with altered leaf morphology. Leaves were reduced in size and
displayed a "mouse-ear" phenotype. The mid-vein was less prominent,
resulting in a palmate venation pattern. The overall plant height of
overexpression lines was reduced due to a decrease in internode length.
Levels of intermediates in the gibberellin (GA) biosynthetic pathway
were altered, and the bioactive GA, GA1, was reduced by
one-half in sense mutants. Accumulation of mRNA for GA 20-oxidase1, a
key biosynthetic enzyme, decreased in overexpression lines. In vitro
tuberization was enhanced under both short- and long-day photoperiods
in several POTH1 overexpression lines. Sense lines
produced more tubers at a faster rate than controls. These results
imply that POTH1 mediates the development of potato by
acting as a negative regulator of GA biosynthesis.
1
This project was supported in part by the Iowa
Agriculture and Home Economics Experiment Station (Ames) and an Iowa
State University SPRIG. This is journal paper no. J-19520 of the Iowa Agriculture and Home Economics Experiment Station (project no. 3701).
2
These two authors made equal contributions to this work.
*
Corresponding author; e-mail djh{at}iastate.edu; fax
515-294-0730.
© 2003 American Society of Plant Biologists
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