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Plant Physiol, May 2003, Vol. 132, pp. 106-117

Overexpression of a Knotted-Like Homeobox Gene of Potato Alters Vegetative Development by Decreasing Gibberellin Accumulation1

Faye M. Rosin,2 Jennifer K. Hart,2 Harry T. Horner, Peter J. Davies, and David J. Hannapel*

Interdepartmental Program in Molecular, Cellular, and Developmental Biology (F.M.R., D.J.H.), Interdepartmental Genetics Major (J.K.H., D.J.H.), Department of Horticulture (F.M.R., J.K.H., D.J.H.), and Department of Botany, Bessey Microscopy Facility (H.T.H.), Iowa State University, Ames, Iowa 50011; and Department of Plant Biology, Cornell University, Ithaca, New York 14853 (P.J.D.)

Potato (Solanum tuberosum) homeobox 1 (POTH1) is a class I homeobox gene isolated from an early-stage tuber cDNA library. The RNA expression pattern of POTH1, unlike that of most other class I knotted-like homeobox genes, is widespread in the cells of both indeterminate and differentiated tissues. Using in situ hybridization, POTH1 transcripts were detected in meristematic cells, leaf primordia, and the vascular procambium of the young stem. Overexpression of POTH1 produced dwarf plants with altered leaf morphology. Leaves were reduced in size and displayed a "mouse-ear" phenotype. The mid-vein was less prominent, resulting in a palmate venation pattern. The overall plant height of overexpression lines was reduced due to a decrease in internode length. Levels of intermediates in the gibberellin (GA) biosynthetic pathway were altered, and the bioactive GA, GA1, was reduced by one-half in sense mutants. Accumulation of mRNA for GA 20-oxidase1, a key biosynthetic enzyme, decreased in overexpression lines. In vitro tuberization was enhanced under both short- and long-day photoperiods in several POTH1 overexpression lines. Sense lines produced more tubers at a faster rate than controls. These results imply that POTH1 mediates the development of potato by acting as a negative regulator of GA biosynthesis.

1 This project was supported in part by the Iowa Agriculture and Home Economics Experiment Station (Ames) and an Iowa State University SPRIG. This is journal paper no. J-19520 of the Iowa Agriculture and Home Economics Experiment Station (project no. 3701).

2 These two authors made equal contributions to this work.

* Corresponding author; e-mail djh{at}iastate.edu; fax 515-294-0730.

© 2003 American Society of Plant Biologists


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