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Plant Physiol, May 2003, Vol. 132, pp. 146-153
Enhancement of Plant-Microbe Interactions Using a Rhizosphere
Metabolomics-Driven Approach and Its Application in the Removal of
Polychlorinated Biphenyls1,[w]
Kothandaraman
Narasimhan,
Chanbasha
Basheer,
Vladimir B.
Bajic, and
Sanjay
Swarup*
Department of Biological Sciences (K.N., S.S.) and Department of
Chemistry (C.B.), 10 Science Drive 4, National University of Singapore,
Singapore 117 543; and Institute for Infocomm Research (I2R), 21 Heng
Mui Keng Terrace, National University of Singapore, Singapore 119 613 (V.B.B.)
Persistent organic pollutants, such as polychlorinated biphenyls
(PCBs), are a global problem. We demonstrate enhanced depletion of PCBs
using root-associated microbes, which can use plant secondary metabolites, such as phenylpropanoids. Using a "rhizosphere
metabolomics" approach, we show that phenylpropanoids constitute 84%
of the secondary metabolites exuded from Arabidopsis roots.
Phenylpropanoid-utilizing microbes are more competitive and are able to
grow at least 100-fold better than their auxotrophic mutants on roots
of plants that are able to synthesize or overproduce phenylpropanoids,
such as flavonoids. Better colonization of the
phenylpropanoid-utilizing strain in a gnotobiotic system on the roots
of flavonoid-producing plants leads to almost 90% removal of PCBs in a
28-d period. Our work complements previous approaches to engineer soil
microbial populations based on opines produced by transgenic plants and used by microbes carrying opine metabolism genes. The current approach
based on plant natural products can be applied to contaminated soils
with pre-existing vegetation. This strategy is also likely to be
applicable to improving the competitive abilities of biocontrol and
biofertilization strains.
1
This work was supported by the Academic Research
Fund, National University of Singapore (grant nos.
R-154-000-064-112 and R-154-000-059-112). K.N. was supported by
a Research Scholarship from the National University of Singapore.
[w]
The online version of this article contains Web-only
data. The supplemental material is available at
www.plantphysiol.org.
*
Corresponding author; e-mail dbsss{at}nus.edu.sg; fax
65-6779-2486.
© 2003 American Society of Plant Biologists
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