First published online April 3, 2003; 10.1104/pp.103.020339
Plant Physiol, May 2003, Vol. 132, pp. 154-160
The Cotton Kinesin-Like Calmodulin-Binding Protein Associates
with Cortical Microtubules in Cotton Fibers1
Mary L.
Preuss,2
Deborah P.
Delmer,3 and
Bo
Liu*
Section of Plant Biology, University of California, One Shields
Avenue, Davis, California 95616
Microtubules in interphase plant cells form a cortical array, which
is critical for plant cell morphogenesis. Genetic studies imply that
the minus end-directed microtubule motor kinesin-like calmodulin-binding protein (KCBP) plays a role in trichome
morphogenesis in Arabidopsis. However, it was not clear whether
this motor interacted with interphase microtubules. In cotton
(Gossypium hirsutum) fibers, cortical microtubules
undergo dramatic reorganization during fiber development. In this
study, cDNA clones of the cotton KCBP homolog GhKCBP were isolated from
a cotton fiber-specific cDNA library. During cotton fiber development
from 10 to 21 DPA, the GhKCBP protein level gradually decreases. By
immunofluorescence, GhKCBP was detected as puncta along cortical
microtubules in fiber cells of different developmental stages. Thus our
results provide evidence that GhKCBP plays a role in interphase cell
growth likely by interacting with cortical microtubules. In contrast to
fibers, in dividing cells of cotton, GhKCBP localized to the nucleus,
the microtubule preprophase band, mitotic spindle, and the
phragmoplast. Therefore KCBP likely exerts multiple roles in cell
division and cell growth in flowering plants.
1
This work was supported by the Division of
Energy Biosciences, U.S. Department of Energy (grant nos.
DE-FG-03-01ER15189 to B.L. and DE-FG-03-963ER20238 to
D.P.D.).
2
Present address: Donald Danforth Plant Science Center,
975 North Warson Road, St. Louis, MO 63132.
3
Present address: The Rockefeller Foundation, 420 Fifth
Avenue, New York, NY 10018-2702.
*
Corresponding author, e-mail bliu{at}ucdavis.edu; fax
530-752-5410.
© 2003 American Society of Plant Biologists
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