|
Plant Physiol, May 2003, Vol. 132, pp. 161-173
A Novel Family in Medicago truncatula Consisting of
More Than 300 Nodule-Specific Genes Coding for Small, Secreted
Polypeptides with Conserved Cysteine Motifs1,[w]
Peter
Mergaert,2
Krisztina
Nikovics,2
Zsolt
Kelemen,
Nicolas
Maunoury,
Danièle
Vaubert,
Adam
Kondorosi, and
Eva
Kondorosi*
Institut des Sciences du Végétal, Centre National de la
Recherche Scientifique, Avenue de la Terrasse, 91198 Gif-sur-Yvette,
France
Transcriptome analysis of Medicago
truncatula nodules has led to the discovery of a gene family
named NCR (nodule-specific cysteine rich) with more than
300 members. The encoded polypeptides were short (60-90 amino acids),
carried a conserved signal peptide, and, except for a conserved
cysteine motif, displayed otherwise extensive sequence divergence.
Family members were found in pea (Pisum sativum), broad
bean (Vicia faba), white clover (Trifolium repens), and Galega orientalis but not in other
plants, including other legumes, suggesting that the family might be
specific for galegoid legumes forming indeterminate nodules. Gene
expression of all family members was restricted to nodules except for
two, also expressed in mycorrhizal roots. NCR genes
exhibited distinct temporal and spatial expression patterns in nodules
and, thus, were coupled to different stages of development. The
signal peptide targeted the polypeptides in the secretory pathway, as
shown by green fluorescent protein fusions expressed in onion
(Allium cepa) epidermal cells. Coregulation of
certain NCR genes with genes coding for a potentially
secreted calmodulin-like protein and for a signal peptide peptidase
suggests a concerted action in nodule development. Potential functions
of the NCR polypeptides in cell-to-cell signaling and creation of a
defense system are discussed.
1
This work was supported in part by "Action
Puces à ADN-Centre National de la Recherche
Scientifique" (grant), by the Centre National de la Recherche
Scientifique-Hungarian Academy of Sciences "Jumelage" program
(fellowships to K.N. and Z.K.), and by the Ministère de la
Recherche et de la Technologie (fellowship to N.M.).
2
These authors contributed equally to the paper.
[w]
The online version of this article contains Web-only
data. The supplemental material is available at
www.plantphysiol.org.
*
Corresponding author; e-mail eva.kondorosi{at}isv.cnrs-gif.fr;
fax 33-1-69-82-36-95.
© 2003 American Society of Plant Biologists
This article has been cited by other articles:
|
|
|
|
 
G. Den Herder, A. De Keyser, R. De Rycke, S. Rombauts, W. Van de Velde, M. R. Clemente, C. Verplancke, P. Mergaert, E. Kondorosi, M. Holsters, et al.
Seven in Absentia Proteins Affect Plant Growth and Nodulation in Medicago truncatula
Plant Physiology,
September 1, 2008;
148(1):
369 - 382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Alkhalfioui, M. Renard, P. Frendo, C. Keichinger, Y. Meyer, E. Gelhaye, M. Hirasawa, D. B. Knaff, C. Ritzenthaler, and F. Montrichard
A Novel Type of Thioredoxin Dedicated to Symbiosis in Legumes
Plant Physiology,
September 1, 2008;
148(1):
424 - 435.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-P. Combier, T. Vernie, F. de Billy, F. El Yahyaoui, R. Mathis, and P. Gamas
The MtMMPL1 Early Nodulin Is a Novel Member of the Matrix Metalloendoproteinase Family with a Role in Medicago truncatula Infection by Sinorhizobium meliloti
Plant Physiology,
June 1, 2007;
144(2):
703 - 716.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-X. Chou, X.-Y. Wei, D.-S. Chen, and J.-C. Zhou
Thirteen nodule-specific or nodule-enhanced genes encoding products homologous to cysteine cluster proteins or plant lipid transfer proteins are identified in Astragalus sinicus L. by suppressive subtractive hybridization
J. Exp. Bot.,
August 1, 2006;
57(11):
2673 - 2685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Van de Velde, J. C. P. Guerra, A. D. Keyser, R. De Rycke, S. Rombauts, N. Maunoury, P. Mergaert, E. Kondorosi, M. Holsters, and S. Goormachtig
Aging in Legume Symbiosis. A Molecular View on Nodule Senescence in Medicago truncatula
Plant Physiology,
June 1, 2006;
141(2):
711 - 720.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Mergaert, T. Uchiumi, B. Alunni, G. Evanno, A. Cheron, O. Catrice, A.-E. Mausset, F. Barloy-Hubler, F. Galibert, A. Kondorosi, et al.
Eukaryotic control on bacterial cell cycle and differentiation in the Rhizobium-legume symbiosis
PNAS,
March 28, 2006;
103(13):
5230 - 5235.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Narvaez-Vasquez, G. Pearce, and C. A. Ryan
The plant cell wall matrix harbors a precursor of defense signaling peptides
PNAS,
September 6, 2005;
102(36):
12974 - 12977.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A.T. Silverstein, M. A. Graham, T. D. Paape, and K. A. VandenBosch
Genome Organization of More Than 300 Defensin-Like Genes in Arabidopsis
Plant Physiology,
June 1, 2005;
138(2):
600 - 610.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zhu, H.-K. Choi, D. R. Cook, and R. C. Shoemaker
Bridging Model and Crop Legumes through Comparative Genomics
Plant Physiology,
April 1, 2005;
137(4):
1189 - 1196.
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Hohnjec, M. F. Vieweg, A. Puhler, A. Becker, and H. Kuster
Overlaps in the Transcriptional Profiles of Medicago truncatula Roots Inoculated with Two Different Glomus Fungi Provide Insights into the Genetic Program Activated during Arbuscular Mycorrhiza
Plant Physiology,
April 1, 2005;
137(4):
1283 - 1301.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Ooki, M. Banba, K. Yano, J. Maruya, S. Sato, S. Tabata, K. Saeki, M. Hayashi, M. Kawaguchi, K. Izui, et al.
Characterization of the Lotus japonicus Symbiotic Mutant lot1 That Shows a Reduced Nodule Number and Distorted Trichomes
Plant Physiology,
April 1, 2005;
137(4):
1261 - 1271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Dresselhaus, S. Amien, M. Marton, A. Strecke, R. Brettschneider, and S. Cordts
TRANSPARENT LEAF AREA1 Encodes a Secreted Proteolipid Required for Anther Maturation, Morphogenesis, and Differentiation during Leaf Development in Maize
PLANT CELL,
March 1, 2005;
17(3):
730 - 745.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Vandepoele and Y. Van de Peer
Exploring the Plant Transcriptome through Phylogenetic Profiling
Plant Physiology,
January 1, 2005;
137(1):
31 - 42.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. El Yahyaoui, H. Kuster, B. Ben Amor, N. Hohnjec, A. Puhler, A. Becker, J. Gouzy, T. Vernie, C. Gough, A. Niebel, et al.
Expression Profiling in Medicago truncatula Identifies More Than 750 Genes Differentially Expressed during Nodulation, Including Many Potential Regulators of the Symbiotic Program
Plant Physiology,
October 1, 2004;
136(2):
3159 - 3176.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Graham, K. A.T. Silverstein, S. B. Cannon, and K. A. VandenBosch
Computational Identification and Characterization of Novel Genes from Legumes
Plant Physiology,
July 1, 2004;
135(3):
1179 - 1197.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. Mitra and S. R. Long
Plant and Bacterial Symbiotic Mutants Define Three Transcriptionally Distinct Stages in the Development of the Medicago truncatula/Sinorhizobium meliloti Symbiosis
Plant Physiology,
February 1, 2004;
134(2):
595 - 604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
