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First published online April 3, 2003; 10.1104/pp.102.018887 Plant Physiol, May 2003, Vol. 132, pp. 185-195 Ethylene Insensitivity Modulates Ozone-Induced Cell Death in Birch1Institute of Biotechnology and Department of Biosciences, University of Helsinki, POB 56 (Viikinkaari 9), FIN-00014 Helsinki, Finland (J.V., R.R., M.K., H.T., J.K.); Department of Applied Biology, University of Helsinki, POB 27 (Latokartanonkaari 5-7), FIN-00014 Helsinki, Finland (J.V.); and Laboratory of Plant Physiology and Molecular Biology, Department of Biology, University of Turku, FIN-20014 Turku, Finland (J.K.)
We have used genotypic variation in birch (Betula
pendula Roth) to investigate the roles of ozone
(O3)-induced ethylene (ET), jasmonic acid, and salicylic
acid in the regulation of tissue tolerance to O3. Of these
hormones, ET evolution correlated best with O3-induced cell
death. Disruption of ET perception by transformation of birch with the
dominant negative mutant allele etr1-1 of the Arabidopsis ET receptor gene ETR1 or blocking of ET
perception with 1-methylcyclopropene reduced but did not completely
prevent the O3-induced cell death, when inhibition of ET
biosynthesis with aminooxyacetic acid completely abolished
O3 lesion formation. This suggests the presence of an
ET-signaling-independent but ET biosynthesis-dependent component in the
ET-mediated stimulation of cell death in O3-exposed birch.
Functional ET signaling was required for the O3 induction
of the gene encoding 1 This work was supported by the Maj and Tor Nessling Foundation, by the Finnish Society of Forest Sciences, by the Leo and Regina Wainstein's Foundation, and by the Academy of Finland, Centre of Excellence Program 2000-2005. M.K. was supported by Academy of Finland Postdoctoral grant no. 48640. 2 Present address: Department of Biology, University of Joensuu, POB 111, FIN-80101 Joensuu, Finland. 3 Present address: Umeå Plant Science Center, Umeå University, 90187 Umeå, Sweden. * Corresponding author; e-mail jaakko.kangasjarvi{at}helsinki.fi; fax 358-2-333-5549. © 2003 American Society of Plant Biologists |
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