Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (90)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kerk, N. M.
Right arrow Articles by Nelson, T. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kerk, N. M.
Right arrow Articles by Nelson, T. M.
Agricola
Right arrow Articles by Kerk, N. M.
Right arrow Articles by Nelson, T. M.

Plant Physiol, May 2003, Vol. 132, pp. 27-35

Laser Capture Microdissection of Cells from Plant Tissues1

Nancy M. Kerk, Teresa Ceserani, S. Lorraine Tausta, Ian M. Sussex, and Timothy M. Nelson*

Department of Molecular, Cellular and Developmental Biology, Yale University, P.O. Box 208104, New Haven, Connecticut 06520-8104

Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, by tacking selected cells to an adhesive film with a laser beam. Harvested cells can provide DNA, RNA, and protein for the profiling of genomic characteristics, gene expression, and protein spectra from individual cell types. We have optimized LCM for a variety of plant tissues and species, permitting the harvesting of cells from paraffin sections that maintain histological detail. We show that RNA can be extracted from LCM-harvested plant cells in amount and quality that are sufficient for the comparison of RNAs among individual cell types. The linear amplification of LCM-captured RNA should permit the expression profiling of plant cell types.

1 This work was supported by the National Science Foundation 2010 Program (grant no. IBN-0114648 to T.N.).

* Corresponding author; e-mail timothy.nelson{at}yale.edu; fax 203-432-5632.

© 2003 American Society of Plant Biologists


This article has been cited by other articles:


Home page
 Plant CellHome page
I. S. Moller, M. Gilliham, D. Jha, G. M. Mayo, S. J. Roy, J. C. Coates, J. Haseloff, and M. Tester
Shoot Na+ Exclusion and Increased Salinity Tolerance Engineered by Cell Type-Specific Alteration of Na+ Transport in Arabidopsis
PLANT CELL, July 1, 2009; 21(7): 2163 - 2178.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Tauris, S. Borg, P. L. Gregersen, and P. B. Holm
A roadmap for zinc trafficking in the developing barley grain based on laser capture microdissection and gene expression profiling
J. Exp. Bot., March 1, 2009; 60(4): 1333 - 1347.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. J. Gardner, A. J. Baker, J.-M. Assie, R. S. Poethig, J. P. Haseloff, and A. A. R. Webb
GAL4 GFP enhancer trap lines for analysis of stomatal guard cell development and gene expression
J. Exp. Bot., January 1, 2009; 60(1): 213 - 226.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Suwabe, G. Suzuki, H. Takahashi, K. Shiono, M. Endo, K. Yano, M. Fujita, H. Masuko, H. Saito, T. Fujioka, et al.
Separated Transcriptomes of Male Gametophyte and Tapetum in Rice: Validity of a Laser Microdissection (LM) Microarray
Plant Cell Physiol., October 1, 2008; 49(10): 1407 - 1416.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Hobo, K. Suwabe, K. Aya, G. Suzuki, K. Yano, T. Ishimizu, M. Fujita, S. Kikuchi, K. Hamada, M. Miyano, et al.
Various Spatiotemporal Expression Profiles of Anther-Expressed Genes in Rice
Plant Cell Physiol., October 1, 2008; 49(10): 1417 - 1428.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Santi and W. Schmidt
Laser microdissection-assisted analysis of the functional fate of iron deficiency-induced root hairs in cucumber
J. Exp. Bot., March 2, 2008; (2008) erm351v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Cai and C. C. Lashbrook
Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing Arabidopsis ZINC FINGER PROTEIN2
Plant Physiology, March 1, 2008; 146(3): 1305 - 1321.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Dembinsky, K. Woll, M. Saleem, Y. Liu, Y. Fu, L. A. Borsuk, T. Lamkemeyer, C. Fladerer, J. Madlung, B. Barbazuk, et al.
Transcriptomic and Proteomic Analyses of Pericycle Cells of the Maize Primary Root
Plant Physiology, November 1, 2007; 145(3): 575 - 588.
[Abstract] [Full Text] [PDF]

Home page
 CSH ProtocolsHome page
K. Ohtsu and P. S. Schnable
Maize Tissue Preparation and Extraction of RNA from Target Cells for Genotyping
CSH Protocols, July 1, 2007; 2007(14): pdb.prot4784 - pdb.prot4784.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
B. H. Le, J. A. Wagmaister, T. Kawashima, A. Q. Bui, J. J. Harada, and R. B. Goldberg
Using Genomics to Study Legume Seed Development
Plant Physiology, June 1, 2007; 144(2): 562 - 574.
[Full Text] [PDF]

Home page
 Genome ResHome page
S. J. Emrich, W. B. Barbazuk, L. Li, and P. S. Schnable
Gene discovery and annotation using LCM-454 transcriptome sequencing
Genome Res., January 1, 2007; 17(1): 69 - 73.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Ohtsu, H. Takahashi, P. S. Schnable, and M. Nakazono
Cell Type-Specific Gene Expression Profiling in Plants by Using a Combination of Laser Microdissection and High-Throughput Technologies
Plant Cell Physiol., January 1, 2007; 48(1): 3 - 7.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
F. J. Corpas, A. Fernandez-Ocana, A. Carreras, R. Valderrama, F. Luque, F. J. Esteban, M. Rodriguez-Serrano, M. Chaki, J. R. Pedrajas, L. M. Sandalio, et al.
The Expression of Different Superoxide Dismutase Forms is Cell-type Dependent in Olive (Olea europaea L.) Leaves
Plant Cell Physiol., July 1, 2006; 47(7): 984 - 994.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Woll, L. A. Borsuk, H. Stransky, D. Nettleton, P. S. Schnable, and F. Hochholdinger
Isolation, Characterization, and Pericycle-Specific Transcriptome Analyses of the Novel Maize Lateral and Seminal Root Initiation Mutant rum1
Plant Physiology, November 1, 2005; 139(3): 1255 - 1267.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J.-Y. Lee, M. Levesque, and P. N. Benfey
High-Throughput RNA Isolation Technologies. New Tools for High-Resolution Gene Expression Profiling in Plant Systems
Plant Physiology, June 1, 2005; 138(2): 585 - 590.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. E. Zanetti, I.-F. Chang, F. Gong, D. W. Galbraith, and J. Bailey-Serres
Immunopurification of Polyribosomal Complexes of Arabidopsis for Global Analysis of Gene Expression
Plant Physiology, June 1, 2005; 138(2): 624 - 635.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C.-W. Tung, K. G. Dwyer, M. E. Nasrallah, and J. B. Nasrallah
Genome-Wide Identification of Genes Expressed in Arabidopsis Pistils Specifically along the Path of Pollen Tube Growth
Plant Physiology, June 1, 2005; 138(2): 977 - 989.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. P. Brandt
Microgenomics: gene expression analysis at the tissue-specific and single-cell levels
J. Exp. Bot., February 1, 2005; 56(412): 495 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
B. C. Meyers, S. S. Tej, T. H. Vu, C. D. Haudenschild, V. Agrawal, S. B. Edberg, H. Ghazal, and S. Decola
The Use of MPSS for Whole-Genome Transcriptional Analysis in Arabidopsis
Genome Res., August 1, 2004; 14(8): 1641 - 1653.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. C. Meyers, D. W. Galbraith, T. Nelson, and V. Agrawal
Methods for Transcriptional Profiling in Plants. Be Fruitful and Replicate
Plant Physiology, June 1, 2004; 135(2): 637 - 652.
[Full Text] [PDF]

Home page
 ScienceHome page
K. Birnbaum, D. E. Shasha, J. Y. Wang, J. W. Jung, G. M. Lambert, D. W. Galbraith, and P. N. Benfey
A Gene Expression Map of the Arabidopsis Root
Science, December 12, 2003; 302(5652): 1956 - 1960.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists