First published online April 3, 2003; 10.1104/pp.102.018143
Plant Physiol, May 2003, Vol. 132, pp. 292-299
Generation and Analysis of an Artificial Gene Dosage Series in
Tomato to Study the Mechanisms by Which the Cloned Quantitative Trait
Locus fw2.2 Controls Fruit Size1
Jiping
Liu,
Bin
Cong, and
Steven D.
Tanksley*
Department of Plant Breeding and Department of Plant Biology,
Cornell University, Ithaca, New York 14853
It has been proposed that fw2.2 encodes a negative
fruit-growth regulator that underlies natural fruit-size variation in
tomato (Lycopersicon spp.) via heterochronic allelic
variation of fw2.2 expression, rather than by variation
in the structural protein itself. To further test the negative
regulator and the transcriptional control hypotheses, a gene dosage
series was constructed, which produced a wider range of
fw2.2 transcript accumulation than can be found in
natural tomato populations. Fruit developmental analyses revealed that
fw2.2 transcript levels were highly correlated
(negatively) with fruit mass, supporting the negative regulator and
transcriptional regulation hypotheses. Further, the effect of
fw2.2 on fruit mass was mediated by repressing three-
and two-dimensional cell division in placental and pericarp tissues,
respectively. Finally, fw2.2 had little effect on
fertility and seed size/number, indicating that fruit size effects of
fw2.2 are due largely to expression in the maternal
tissues of developing fruit and not mediated through fertility or
seed-setting-related processes.
1
This work was supported by the National Science
Foundation (grant no. DBI-0116076), by the U.S. Department of
Agriculture Plant Genome Program (grant no. 97-35300-4384), and by
the U.S.-Israel Binational Agriculture Research and Development Fund
(grant no. IS-3009-98C).
*
Corresponding author; e-mail sdt4{at}cornell.edu; fax
607-255-6683.
© 2003 American Society of Plant Biologists
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