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Plant Physiol, May 2003, Vol. 132, pp. 36-43
Genetic Modification Removes an Immunodominant Allergen from
Soybean1,[w]
Eliot M.
Herman,
Ricki M.
Helm,
Rudolf
Jung, and
Anthony J.
Kinney*
Plant Genetics Research Unit, United States Department of
Agriculture/Agricultural Research Service, Donald Danforth Plant
Science Center, 975 North Warson Street, St. Louis, Missouri 63132 (E.M.H.); University of Arkansas for Medical Sciences, Arkansas
Children's Hospital Research Institute, 1120 Marshall Street, Little
Rock, Arkansas 72202 (R.M.H.); Pioneer Hi-Bred International, 7300 NW
62nd Avenue, Johnston, Iowa 50131-1004 (R.J.); and Dupont Experimental
Station, P.O. Box 80402, Wilmington, Delaware 19880-0402
(A.J.K.)
The increasing use of soybean (Glycine max)
products in processed foods poses a potential threat to
soybean-sensitive food-allergic individuals. In vitro assays on soybean
seed proteins with sera from soybean-sensitive individuals have
immunoglobulin E reactivity to abundant storage proteins and a few
less-abundant seed proteins. One of these low abundance proteins, Gly m
Bd 30 K, also referred to as P34, is in fact a major (i.e.
immunodominant) soybean allergen. Although a member of the papain
protease superfamily, Gly m Bd 30 K has a glycine in the conserved
catalytic cysteine position found in all other cysteine proteases.
Transgene-induced gene silencing was used to prevent the accumulation
of Gly m Bd 30 K protein in soybean seeds. The Gly m Bd 30 K-silenced
plants and their seeds lacked any compositional, developmental,
structural, or ultrastructural phenotypic differences when compared
with control plants. Proteomic analysis of extracts from transgenic
seed detected the suppression of Gly m Bd 30 K-related peptides but no
other significant changes in polypeptide pattern. The lack of a
collateral alteration of any other seed protein in the Gly m Bd 30 K-silenced seeds supports the presumption that the protein does not
have a role in seed protein processing and maturation. These data
provide evidence for substantial equivalence of composition of
transgenic and non-transgenic seed eliminating one of the dominant
allergens of soybean seeds.
1
This work was supported in part by the U.S.
Department of Agriculture Initiative for Future Food and Agricultural
Systems (grant no. 2001-04239 to R.M.H. and E.M.H.).
[w]
The online version of this article contains Web-only
data. The supplemental material is available at
www.plantphysiol.org.
*
Corresponding author; e-mail
anthony.kinney{at}usa.dupont.com; fax 302-695-9149.
© 2003 American Society of Plant Biologists
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