|
|
||||||||
|
Plant Physiol, May 2003, Vol. 132, pp. 64-74 Expression Profiling and Bioinformatic Analyses of a Novel Stress-Regulated Multispanning Transmembrane Protein Family from Cereals and Arabidopsis1,[w]Département des Sciences biologiques, Université du Québec à Montréal, Case Postale 8888, succursale Centre-ville, Montréal, Canada H3C 3P8
Cold acclimation is a multigenic trait that allows hardy
plants to develop efficient tolerance mechanisms needed for winter survival. To determine the genetic nature of these mechanisms, several
cold-responsive genes of unknown function were identified from
cold-acclimated wheat (Triticum aestivum). To identify
the putative functions and structural features of these new genes, integrated genomic approaches of data mining, expression profiling, and
bioinformatic predictions were used. The analyses revealed that one of
these genes is a member of a small family that encodes two distinct
groups of multispanning transmembrane proteins. The cold-regulated
(COR)413-plasma membrane and COR413-thylakoid membrane groups are
potentially targeted to the plasma membrane and thylakoid membrane,
respectively. Further sequence analysis of the two groups from
different plant species revealed the presence of a highly conserved
phosphorylation site and a glycosylphosphatidylinositol-anchoring site
at the C-terminal end. No homologous sequences were found in other
organisms suggesting that this family is specific to the plant kingdom.
Intraspecies and interspecies comparative gene expression profiling
shows that the expression of this gene family is correlated with the
development of freezing tolerance in cereals and Arabidopsis. In
addition, several members of the family are regulated by water stress,
light, and abscisic acid. Structure predictions and comparative genome
analyses allow us to propose that the cor413 genes
encode putative G-protein-coupled receptors.
1 This work was supported by the Natural Sciences and Engineering Research Council of Canada, by Genome Canada, by Genome Québec, and by Fonds pour la Formation des Chercheurs et l'Aide à la recherche (research grants to F.S.). [w] The online version of this article contains Web-only data. The supplemental material is available at www.plantphysiol.org. * Corresponding author; e-mail sarhan.fathey{at}uqam.ca; fax 514-987-4647. © 2003 American Society of Plant Biologists This article has been cited by other articles:
|
|||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ASPB Publications | PLANT PHYSIOLOGY® | THE PLANT CELL | |
|---|---|---|---|