First published online May 15, 2003; 10.1104/pp.102.017814
Plant Physiology 132:1020-1032 (2003)
© 2003 American Society of Plant Biologists
CELL BIOLOGY AND SIGNAL TRANSDUCTION
A Role for the GCC-Box in Jasmonate-Mediated Activation of the PDF1.2 Gene of Arabidopsis1
Rebecca L. Brown,
Kemal Kazan,
Ken C. McGrath,
Don J. Maclean and
John M. Manners
Cooperative Research Centre for Tropical Plant Protection (R.L.B., K.K., K.C.M., D.J.M., J.M.M.), and Department of Biochemistry and Molecular Biology (R.L.B., K.M., D.J.M.), The University of Queensland, Brisbane, Queensland 4072, Australia; and Commonwealth Scientific and Industrial Research Organisation, Plant Industry, Queensland Bioscience Precinct, St. Lucia, Brisbane, Queensland 4067, Australia (K.K., J.M.M.)
The PDF1.2 gene of Arabidopsis encoding a plant defensin is commonly used as a marker for characterization of the jasmonate-dependent defense responses. Here, using PDF1.2 promoter-deletion lines linked to the  -glucoronidase-reporter gene, we examined putative promoter elements associated with jasmonate-responsive expression of this gene. Using stably transformed plants, we first characterized the extended promoter region that positively regulates basal expression from the PDF1.2 promoter. Second, using promoter deletion constructs including one from which the GCC-box region was deleted, we observed a substantially lower response to jasmonate than lines carrying this motif. In addition, point mutations introduced into the core GCC-box sequence substantially reduced jasmonate responsiveness, whereas addition of a 20-nucleotide-long promoter element carrying the core GCC-box and flanking nucleotides provided jasmonate responsiveness to a 35S minimal promoter. Taken together, these results indicated that the GCC-box plays a key role in conferring jasmonate responsiveness to the PDF1.2 promoter. However, deletion or specific mutations introduced into the core GCC-box did not completely abolish the jasmonate responsiveness of the promoter, suggesting that the other promoter elements lying downstream from the GCC-box region may also contribute to jasmonate responsiveness. In other experiments, we identified a jasmonate- and pathogen-responsive ethylene response factor transcription factor, AtERF2, which when overexpressed in transgenic Arabidopsis plants activated transcription from the PDF1.2, Thi2.1, and PR4 (basic chitinase) genes, all of which contain a GCC-box sequence in their promoters. Our results suggest that in addition to their roles in regulating ethylene-mediated gene expression, ethylene response factors also appear to play important roles in regulating jasmonate-responsive gene expression, possibly via interaction with the GCC-box.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.102.017814.
1 This work was supported by the Grains Research and Development Corporation (postgraduate fellowship to R.L.B.).
* Corresponding author; e-mail kemal.kazan{at}csiro.au; fax 61–7–3214–2950.
Received November 14, 2002;
returned for revision January 4, 2003;
accepted March 5, 2003.
This article has been cited by other articles:
|
|
|
|
 
A. Hayward, P. Stirnberg, C. Beveridge, and O. Leyser
Interactions between Auxin and Strigolactone in Shoot Branching Control
Plant Physiology,
September 1, 2009;
151(1):
400 - 412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Moreno, Y. Tao, J. Chory, and C. L. Ballare
Ecological modulation of plant defense via phytochrome control of jasmonate sensitivity
PNAS,
March 24, 2009;
106(12):
4935 - 4940.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kumar, W. Busch, H. Birke, B. Kemmerling, T. Nurnberger, and F. Schoffl
Heat Shock Factors HsfB1 and HsfB2b Are Involved in the Regulation of Pdf1.2 Expression and Pathogen Resistance in Arabidopsis
Mol Plant,
January 1, 2009;
2(1):
152 - 165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Xiang, N. Tang, H. Du, H. Ye, and L. Xiong
Characterization of OsbZIP23 as a Key Player of the Basic Leucine Zipper Transcription Factor Family for Conferring Abscisic Acid Sensitivity and Salinity and Drought Tolerance in Rice
Plant Physiology,
December 1, 2008;
148(4):
1938 - 1952.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Gonzalez-Lamothe, P. Boyle, A. Dulude, V. Roy, C. Lezin-Doumbou, G. S. Kaur, K. Bouarab, C. Despres, and N. Brisson
The Transcriptional Activator Pti4 Is Required for the Recruitment of a Repressosome Nucleated by Repressor SEBF at the Potato PR-10a Gene
PLANT CELL,
November 1, 2008;
20(11):
3136 - 3147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Krom and W. Ramakrishna
Comparative Analysis of Divergent and Convergent Gene Pairs and Their Expression Patterns in Rice, Arabidopsis, and Populus
Plant Physiology,
August 1, 2008;
147(4):
1763 - 1773.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Pre, M. Atallah, A. Champion, M. De Vos, C. M. J. Pieterse, and J. Memelink
The AP2/ERF Domain Transcription Factor ORA59 Integrates Jasmonic Acid and Ethylene Signals in Plant Defense
Plant Physiology,
July 1, 2008;
147(3):
1347 - 1357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Grennan
Ethylene Response Factors in Jasmonate Signaling and Defense Response
Plant Physiology,
April 1, 2008;
146(4):
1457 - 1458.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sun, J.-P. Yu, F. Chen, T.-J. Zhao, X.-H. Fang, Y.-Q. Li, and S.-F. Sui
TINY, a Dehydration-responsive Element (DRE)-binding Protein-like Transcription Factor Connecting the DRE- and Ethylene-responsive Element-mediated Signaling Pathways in Arabidopsis
J. Biol. Chem.,
March 7, 2008;
283(10):
6261 - 6271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Andriankaja, A. Boisson-Dernier, L. Frances, L. Sauviac, A. Jauneau, D. G. Barker, and F. de Carvalho-Niebel
AP2-ERF Transcription Factors Mediate Nod Factor Dependent Mt ENOD11 Activation in Root Hairs via a Novel cis-Regulatory Motif
PLANT CELL,
September 1, 2007;
19(9):
2866 - 2885.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Li, W. Xu, W. Yang, Z. Kong, and Y. Xue
Genome-Wide Gene Expression Profiling Reveals Conserved and Novel Molecular Functions of the Stigma in Rice
Plant Physiology,
August 1, 2007;
144(4):
1797 - 1812.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Dombrecht, G. P. Xue, S. J. Sprague, J. A. Kirkegaard, J. J. Ross, J. B. Reid, G. P. Fitt, N. Sewelam, P. M. Schenk, J. M. Manners, et al.
MYC2 Differentially Modulates Diverse Jasmonate-Dependent Functions in Arabidopsis
PLANT CELL,
July 1, 2007;
19(7):
2225 - 2245.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Vom Endt, M. Soares e Silva, J. W. Kijne, G. Pasquali, and J. Memelink
Identification of a Bipartite Jasmonate-Responsive Promoter Element in the Catharanthus roseus ORCA3 Transcription Factor Gene That Interacts Specifically with AT-Hook DNA-Binding Proteins
Plant Physiology,
July 1, 2007;
144(3):
1680 - 1689.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Freeling, L. Rapaka, E. Lyons, B. Pedersen, and B. C. Thomas
G-Boxes, Bigfoot Genes, and Environmental Response: Characterization of Intragenomic Conserved Noncoding Sequences in Arabidopsis
PLANT CELL,
May 1, 2007;
19(5):
1441 - 1457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Pirrello, F. Jaimes-Miranda, M. T. Sanchez-Ballesta, B. Tournier, Q. Khalil-Ahmad, F. Regad, A. Latche, J. C. Pech, and M. Bouzayen
Sl-ERF2, a Tomato Ethylene Response Factor Involved in Ethylene Response and Seed Germination
Plant Cell Physiol.,
September 1, 2006;
47(9):
1195 - 1205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. C. McGrath, B. Dombrecht, J. M. Manners, P. M. Schenk, C. I. Edgar, D. J. Maclean, W.-R. Scheible, M. K. Udvardi, and K. Kazan
Repressor- and Activator-Type Ethylene Response Factors Functioning in Jasmonate Signaling and Disease Resistance Identified via a Genome-Wide Screen of Arabidopsis Transcription Factor Gene Expression
Plant Physiology,
October 1, 2005;
139(2):
949 - 959.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-P. Song, M. Agarwal, M. Ohta, Y. Guo, U. Halfter, P. Wang, and J.-K. Zhu
Role of an Arabidopsis AP2/EREBP-Type Transcriptional Repressor in Abscisic Acid and Drought Stress Responses
PLANT CELL,
August 1, 2005;
17(8):
2384 - 2396.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. W. Wilson, G. C. Kennedy, J. W. Peacock, and E. S. Dennis
Microarray Analysis Reveals Vegetative Molecular Phenotypes of Arabidopsis Flowering-time Mutants
Plant Cell Physiol.,
August 1, 2005;
46(8):
1190 - 1201.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ogawa, L. Pan, M. Kawai-Yamada, L.-H. Yu, S. Yamamura, T. Koyama, S. Kitajima, M. Ohme-Takagi, F. Sato, and H. Uchimiya
Functional Analysis of Arabidopsis Ethylene-Responsive Element Binding Protein Conferring Resistance to Bax and Abiotic Stress-Induced Plant Cell Death
Plant Physiology,
July 1, 2005;
138(3):
1436 - 1445.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Anderson, E. Badruzsaufari, P. M. Schenk, J. M. Manners, O. J. Desmond, C. Ehlert, D. J. Maclean, P. R. Ebert, and K. Kazan
Antagonistic Interaction between Abscisic Acid and Jasmonate-Ethylene Signaling Pathways Modulates Defense Gene Expression and Disease Resistance in Arabidopsis
PLANT CELL,
December 1, 2004;
16(12):
3460 - 3479.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. D. Buchanan, P. E. Klein, and J. E. Mullet
Phylogenetic Analysis of 5'-Noncoding Regions From the ABA-Responsive rab16/17 Gene Family of Sorghum, Maize and Rice Provides Insight Into the Composition, Organization and Function of cis-Regulatory Modules
Genetics,
November 1, 2004;
168(3):
1639 - 1654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Boter, O. Ruiz-Rivero, A. Abdeen, and S. Prat
Conserved MYC transcription factors play a key role in jasmonate signaling both in tomato and Arabidopsis
Genes & Dev.,
July 1, 2004;
18(13):
1577 - 1591.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Gachon, A. Mingam, and B. Charrier
Real-time PCR: what relevance to plant studies?
J. Exp. Bot.,
July 1, 2004;
55(402):
1445 - 1454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Lorenzo, J. M. Chico, J. J. Sanchez-Serrano, and R. Solano
JASMONATE-INSENSITIVE1 Encodes a MYC Transcription Factor Essential to Discriminate between Different Jasmonate-Regulated Defense Responses in Arabidopsis
PLANT CELL,
July 1, 2004;
16(7):
1938 - 1950.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhao, S.-H. Zheng, K. Fujita, and K. Sakai
Jasmonate and ethylene signalling and their interaction are integral parts of the elicitor signalling pathway leading to {beta}-thujaplicin biosynthesis in Cupressus lusitanica cell cultures
J. Exp. Bot.,
May 1, 2004;
55(399):
1003 - 1012.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. J. Campbell, P. M. Schenk, K. Kazan, I. A.M.A. Penninckx, J. P. Anderson, D. J. Maclean, B. P.A. Cammue, P. R. Ebert, and J. M. Manners
Pathogen-Responsive Expression of a Putative ATP-Binding Cassette Transporter Gene Conferring Resistance to the Diterpenoid Sclareol Is Regulated by Multiple Defense Signaling Pathways in Arabidopsis
Plant Physiology,
November 1, 2003;
133(3):
1272 - 1284.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
