Plant Physiology 132:485-493 (2003)
© 2003 American Society of Plant Biologists
RESEARCH PAPERS ON SYSTEMS BIOLOGY/GENOMICS/BIOINFORMATICS
Rapid, Noninvasive Screening for Perturbations of Metabolism and Plant Growth Using Chlorophyll Fluorescence Imaging1
Romina P. Barbagallo,
Kevin Oxborough,
Kenneth E. Pallett and
Neil R. Baker*
Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, United Kingdom (R.P.B., K.O., N.R.B.); and Bayer CropScience GmbH, Ecotoxicology, Industriepark Höchst, 65926 Frankfurt am Main, Germany (K.E.P.)
A rapid, noninvasive technique involving imaging of chlorophyll fluorescence parameters for detecting perturbations of leaf metabolism and growth in seedlings is described. Arabidopsis seedlings were grown in 96-well microtitre plates for 4 d and then treated with eight herbicides with differing modes of action to induce perturbations in a range of different metabolic processes. Imaging of chlorophyll fluorescence emissions from 96 seedlings growing on a microtitre plate enabled images of a number of fluorescence parameters to be rapidly and simultaneously produced for the plants in each well. Herbicideinduced perturbations in metabolism, even in metabolic reactions not directly associated with photosynthetic metabolism, were detected from the changes in the images of fluorescence parameters considerably before any visual effects on seedling growth were observed. Evaluations of seedling growth were made from measurements of the area of chlorophyll fluorescence emission in images of plants growing in the 96-well plates. Decreased seedling growth related directly to herbicideinduced changes in the imaged chlorophyll fluorescence parameters. The applicability of this rapid-screening technique for metabolic perturbations in monocotyledonous species was demonstrated by treating Agrostis tenuis seedlings with Imazapyr, an inhibitor of branched-chain amino acid synthesis.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.102.018093.
1 This work was supported by the European Commission under the Marie Curie Industrial Host Fellowship Program (grant no. HPMI–CT–1999–00085 to Bayer CropScience GmbH).
* Corresponding author; e-mail baken{at}essex.ac.uk; fax 44–1206–873416.
Received November 22, 2002;
returned for revision January 7, 2003;
accepted February 8, 2003.
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