Plant Physiology 132:517-529 (2003)
© 2003 American Society of Plant Biologists
RESEARCH PAPERS ON SYSTEMS BIOLOGY/GENOMICS/BIOINFORMATICS
Use of Serial Analysis of Gene Expression Technology to Reveal Changes in Gene Expression in Arabidopsis Pollen Undergoing Cold Stress1,[w]
Ji-Yeon Lee and
Dong-Hee Lee*
Department of Life Sciences, Ewha Woman's University, Seoul 120–750, Korea
We have characterized the global gene expression patterns of Arabidopsis pollen using Serial Analysis of Gene Expression (SAGE). A total of 21,237 SAGE tags were sequenced and 4,211 unique tags were identified. Interestingly, the number of unique tags in pollen was low compared with the SAGE library of the leaf constructed on a similar scale. The transcript profiles of pollen reflect accurately the characteristics of pollen as a reproductive organ. Functional classification of the expressed genes reveals that those involved in cellular biogenesis such as polygalacturonase, pectate lyase, and pectin methylesterase make up more than 40% of the total transcripts. However, genes involved in energy and protein synthesis, which are prevalent in leaves, were expressed at a relatively low level. The expression level of the great majority of transcripts was unaffected by cold treatment at 0°C for 72 h, whereas pollen tube growth and seed production were substantially reduced. Interestingly, many genes thought to be responsible for cold acclimation such as COR, lipid transfer protein, and  -amylase, that are highly induced in Arabidopsis leaves, were only expressed at their normal level or weakly induced in the pollen. The expression patterns of the cold-responsive transcripts identified by SAGE were confirmed by microarray analysis. Our results strongly suggest that poor accumulation of proteins that play a role in stress tolerance may be why Arabidopsis pollen is cold sensitive.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.020511.
1 This research was supported in part by Korea Science and Engineering Foundation (grant no. 98–0401–06–01–3), by the Brain Korea 21 Project in 2001, and by the 21st Frontier R&D Program (grant no. CG1122).
[w] The online version of this article contains Web-only data. The supplemental material is available at http://www.plantphysiol.org.
* Corresponding author; e-mail lee{at}ewha.ac.kr; fax 82–2–3277–2385.
Received January 16, 2003;
returned for revision February 25, 2003;
accepted March 19, 2003.
This article has been cited by other articles:
|
|
|
|
 
G. Y. Busot, B. McClure, C. P. Ibarra-Sanchez, K. Jimenez-Duran, S. Vazquez-Santana, and F. Cruz-Garcia
Pollination in Nicotiana alata stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue
J. Exp. Bot.,
August 1, 2008;
59(11):
3187 - 3201.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ganeshan, P. Vitamvas, D. B. Fowler, and R. N. Chibbar
Quantitative expression analysis of selected COR genes reveals their differential expression in leaf and crown tissues of wheat (Triticum aestivum L.) during an extended low temperature acclimation regimen
J. Exp. Bot.,
June 1, 2008;
59(9):
2393 - 2402.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Solecka, J. Zebrowski, and A. Kacperska
Are Pectins Involved in Cold Acclimation and De-acclimation of Winter Oil-seed Rape Plants?
Ann. Bot.,
March 1, 2008;
101(4):
521 - 530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Becker and J. A. Feijo
How Many Genes are Needed to Make a Pollen Tube? Lessons from Transcriptomics
Ann. Bot.,
November 1, 2007;
100(6):
1117 - 1123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Malik, F. Wang, J. M. Dirpaul, N. Zhou, P. L. Polowick, A. M.R. Ferrie, and J. E. Krochko
Transcript Profiling and Identification of Molecular Markers for Early Microspore Embryogenesis in Brassica napus
Plant Physiology,
May 1, 2007;
144(1):
134 - 154.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Dai, T. Chen, K. Chong, Y. Xue, S. Liu, and T. Wang
Proteomics Identification of Differentially Expressed Proteins Associated with Pollen Germination and Tube Growth Reveals Characteristics of Germinated Oryza sativa Pollen
Mol. Cell. Proteomics,
February 1, 2007;
6(2):
207 - 230.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hewezi, M. Leger, W. El Kayal, and L. Gentzbittel
Transcriptional profiling of sunflower plants growing under low temperatures reveals an extensive down-regulation of gene expression associated with chilling sensitivity
J. Exp. Bot.,
September 1, 2006;
57(12):
3109 - 3122.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bao, S. Lee, C. Chen, X. Zhang, Y. Zhang, S. Liu, T. Clark, J. Wang, M. Cao, H. Yang, et al.
Serial Analysis of Gene Expression Study of a Hybrid Rice Strain (LYP9) and Its Parental Cultivars
Plant Physiology,
July 1, 2005;
138(3):
1216 - 1231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Salmi, T. J. Bushart, S. C. Stout, and S. J. Roux
Profile and Analysis of Gene Expression Changes during Early Development in Germinating Spores of Ceratopteris richardii
Plant Physiology,
July 1, 2005;
138(3):
1734 - 1745.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Poroyko, L.G. Hejlek, W.G. Spollen, G.K. Springer, H.T. Nguyen, R.E. Sharp, and H.J. Bohnert
The Maize Root Transcriptome by Serial Analysis of Gene Expression
Plant Physiology,
July 1, 2005;
138(3):
1700 - 1710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Pina, F. Pinto, J. A. Feijo, and J. D. Becker
Gene Family Analysis of the Arabidopsis Pollen Transcriptome Reveals Biological Implications for Cell Growth, Division Control, and Gene Expression Regulation
Plant Physiology,
June 1, 2005;
138(2):
744 - 756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-L. Wang, C.-M. Hsu, L.-C. Chang, C.-S. Wang, T.-H. Su, Y.-J. J. Huang, L. Jiang, and G.-Y. Jauh
Gene Expression Profiles of Cold-stored and Fresh Pollen to Investigate Pollen Germination and Growth
Plant Cell Physiol.,
October 15, 2004;
45(10):
1519 - 1528.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Robinson, D. J. Cram, C. T. Lewis, and I. A.P. Parkin
Maximizing the Efficacy of SAGE Analysis Identifies Novel Transcripts in Arabidopsis
Plant Physiology,
October 1, 2004;
136(2):
3223 - 3233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Munos, C. Cazettes, C. Fizames, F. Gaymard, P. Tillard, M. Lepetit, L. Lejay, and A. Gojon
Transcript Profiling in the chl1-5 Mutant of Arabidopsis Reveals a Role of the Nitrate Transporter NRT1.1 in the Regulation of Another Nitrate Transporter, NRT2.1
PLANT CELL,
September 1, 2004;
16(9):
2433 - 2447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Lalanne, C. Michaelidis, J. M. Moore, W. Gagliano, A. Johnson, R. Patel, R. Howden, J.-P. Vielle-Calzada, U. Grossniklaus, and D. Twell
Analysis of Transposon Insertion Mutants Highlights the Diversity of Mechanisms Underlying Male Progamic Development in Arabidopsis
Genetics,
August 1, 2004;
167(4):
1975 - 1986.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. C. Meyers, D. W. Galbraith, T. Nelson, and V. Agrawal
Methods for Transcriptional Profiling in Plants. Be Fruitful and Replicate
Plant Physiology,
June 1, 2004;
135(2):
637 - 652.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. McCormick
Control of Male Gametophyte Development
PLANT CELL,
June 1, 2004;
16(suppl_1):
S142 - S153.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Fizames, S. Munos, C. Cazettes, P. Nacry, J. Boucherez, F. Gaymard, D. Piquemal, V. Delorme, T. Commes, P. Doumas, et al.
The Arabidopsis Root Transcriptome by Serial Analysis of Gene Expression. Gene Identification Using the Genome Sequence
Plant Physiology,
January 1, 2004;
134(1):
67 - 80.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Chakravarthy, R. P. Tuori, M. D. D'Ascenzo, P. R. Fobert, C. Despres, and G. B. Martin
The Tomato Transcription Factor Pti4 Regulates Defense-Related Gene Expression via GCC Box and Non-GCC Box cis Elements
PLANT CELL,
December 1, 2003;
15(12):
3033 - 3050.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
