This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 132/2/606    most recent pp.103.022186v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (71) Citing Articles via Google Scholar Google Scholar Articles by van Wees, S. C.M. Articles by Glazebrook, J. Search for Related Content PubMed PubMed Citation Articles by van Wees, S. C.M. Articles by Glazebrook, J. Agricola Articles by van Wees, S. C.M. Articles by Glazebrook, J.

RESEARCH PAPERS ON SYSTEMS BIOLOGY/GENOMICS/BIOINFORMATICS

Characterization of the Early Response of Arabidopsis to Alternaria brassicicola Infection Using Expression Profiling^[w]

Torrey Mesa Research Institute, Syngenta Research and Technology, 3115 Merryfield Row, San Diego, California 92121

All tested accessions of Arabidopsis are resistant to the fungal pathogen Alternaria brassicicola. Resistance is compromised by pad3 or coi1 mutations, suggesting that it requires the Arabidopsis phytoalexin camalexin and jasmonic acid (JA)-dependent signaling, respectively. This contrasts with most well-studied Arabidopsis pathogens, which are controlled by salicylic acid-dependent responses and do not benefit from absence of camalexin or JA. Here, mutants with defects in camalexin synthesis (pad1, pad2, pad3, and pad5) or in JA signaling (pad1, coi1) were found to be more susceptible than wild type. Mutants with defects in salicylic acid (pad4 and sid2) or ethylene (ein2) signaling remained resistant. Plant responses to A. brassicicola were characterized using expression profiling. Plants showed dramatic gene expression changes within 12 h, persisting at 24 and 36 h. Wild-type and pad3 plants responded similarly, suggesting that pad3 does not have a major effect on signaling. The response of coi1 plants was quite different. Of the 645 genes induced by A. brassicicola in wild-type and pad3 plants, 265 required COI1 for full expression. It is likely that some of the COI1-dependent genes are important for resistance to A. brassicicola. Responses to A. brassicicola were compared with responses to Pseudomonas syringae infection. Despite the fact that these pathogens are limited by different defense responses, approximately 50% of the induced genes were induced in response to both pathogens. Among these, requirements for COI1 were consistent after infection by either pathogen, suggesting that the regulatory effect of COI1 is similar regardless of the initial stimulus.

^[w] The online version of this article contains Web-only data. The supplemental material is available at http://www.plantphysiol.org.

¹ Present address: The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037.

² Present address: Diversa Corporation, 4955 Directors Place, San Diego, CA 92121.

³ Present address: Syngenta Biotechnology Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709.

^* Corresponding author; e-mail janeg9{at}hotmail.com; fax 858–350–6306.

Received February 12, 2003; returned for revision March 14, 2003; accepted March 14, 2003.

This article has been cited by other articles:

				M. Pogany, U. von Rad, S. Grun, A. Dongo, A. Pintye, P. Simoneau, G. Bahnweg, L. Kiss, B. Barna, and J. Durner Dual Roles of Reactive Oxygen Species and NADPH Oxidase RBOHD in an Arabidopsis-Alternaria Pathosystem Plant Physiology, November 1, 2009; 151(3): 1459 - 1475. [Abstract] [Full Text] [PDF]

				R. Dhawan, H. Luo, A. M. Foerster, S. AbuQamar, H.-N. Du, S. D. Briggs, O. M. Scheid, and T. Mengiste HISTONE MONOUBIQUITINATION1 Interacts with a Subunit of the Mediator Complex and Regulates Defense against Necrotrophic Fungal Pathogens in Arabidopsis PLANT CELL, March 1, 2009; 21(3): 1000 - 1019. [Abstract] [Full Text] [PDF]

				S. C. Lee, I. S. Hwang, H. W. Choi, and B. K. Hwang Involvement of the Pepper Antimicrobial Protein CaAMP1 Gene in Broad Spectrum Disease Resistance Plant Physiology, October 1, 2008; 148(2): 1004 - 1020. [Abstract] [Full Text] [PDF]

				S. AbuQamar, M.-F. Chai, H. Luo, F. Song, and T. Mengiste Tomato Protein Kinase 1b Mediates Signaling of Plant Responses to Necrotrophic Fungi and Insect Herbivory PLANT CELL, July 1, 2008; 20(7): 1964 - 1983. [Abstract] [Full Text] [PDF]

				M. Herde, K. Gartner, T. G. Kollner, B. Fode, W. Boland, J. Gershenzon, C. Gatz, and D. Tholl Identification and Regulation of TPS04/GES, an Arabidopsis Geranyllinalool Synthase Catalyzing the First Step in the Formation of the Insect-Induced Volatile C16-Homoterpene TMTT PLANT CELL, April 1, 2008; 20(4): 1152 - 1168. [Abstract] [Full Text] [PDF]

				J. Wan, X.-C. Zhang, D. Neece, K. M. Ramonell, S. Clough, S.-y. Kim, M. G. Stacey, and G. Stacey A LysM Receptor-Like Kinase Plays a Critical Role in Chitin Signaling and Fungal Resistance in Arabidopsis PLANT CELL, February 1, 2008; 20(2): 471 - 481. [Abstract] [Full Text] [PDF]

				S. H. Spoel, J. S. Johnson, and X. Dong Regulation of tradeoffs between plant defenses against pathogens with different lifestyles PNAS, November 20, 2007; 104(47): 18842 - 18847. [Abstract] [Full Text] [PDF]

				Y. Li, F. Beisson, A. J. K. Koo, I. Molina, M. Pollard, and J. Ohlrogge Identification of acyltransferases required for cutin biosynthesis and production of cutin with suberin-like monomers PNAS, November 13, 2007; 104(46): 18339 - 18344. [Abstract] [Full Text] [PDF]

				M. Nafisi, S. Goregaoker, C. J. Botanga, E. Glawischnig, C. E. Olsen, B. A. Halkier, and J. Glazebrook Arabidopsis Cytochrome P450 Monooxygenase 71A13 Catalyzes the Conversion of Indole-3-Acetaldoxime in Camalexin Synthesis PLANT CELL, June 1, 2007; 19(6): 2039 - 2052. [Abstract] [Full Text] [PDF]

				Y. Trusov, J. E. Rookes, K. Tilbrook, D. Chakravorty, M. G. Mason, D. Anderson, J.-G. Chen, A. M. Jones, and J. R. Botella Heterotrimeric G Protein {gamma} Subunits Provide Functional Selectivity in G{beta}{gamma} Dimer Signaling in Arabidopsis PLANT CELL, April 1, 2007; 19(4): 1235 - 1250. [Abstract] [Full Text] [PDF]

				L. Calo, I. Garcia, C. Gotor, and L. C. Romero Leaf hairs influence phytopathogenic fungus infection and confer an increased resistance when expressing a Trichoderma {alpha}-1,3-glucanase J. Exp. Bot., November 1, 2006; 57(14): 3911 - 3920. [Abstract] [Full Text] [PDF]

				M. De Vos, W. Van Zaanen, A. Koornneef, J. P. Korzelius, M. Dicke, L.C. Van Loon, and C. M.J. Pieterse Herbivore-Induced Resistance against Microbial Pathogens in Arabidopsis Plant Physiology, September 1, 2006; 142(1): 352 - 363. [Abstract] [Full Text] [PDF]

				A. F. Bent, T. K. Hoffman, J. S. Schmidt, G. L. Hartman, D. D. Hoffman, P. Xue, and M. L. Tucker Disease- and Performance-Related Traits of Ethylene-Insensitive Soybean Crop Sci., February 24, 2006; 46(2): 893 - 901. [Abstract] [Full Text] [PDF]

				P. Veronese, H. Nakagami, B. Bluhm, S. AbuQamar, X. Chen, J. Salmeron, R. A. Dietrich, H. Hirt, and T. Mengiste The Membrane-Anchored BOTRYTIS-INDUCED KINASE1 Plays Distinct Roles in Arabidopsis Resistance to Necrotrophic and Biotrophic Pathogens PLANT CELL, January 1, 2006; 18(1): 257 - 273. [Abstract] [Full Text] [PDF]

				Y. Trusov, J. E. Rookes, D. Chakravorty, D. Armour, P. M. Schenk, and J. R. Botella Heterotrimeric G Proteins Facilitate Arabidopsis Resistance to Necrotrophic Pathogens and Are Involved in Jasmonate Signaling Plant Physiology, January 1, 2006; 140(1): 210 - 220. [Abstract] [Full Text] [PDF]

				C. Zhou, L. Zhang, J. Duan, B. Miki, and K. Wu HISTONE DEACETYLASE19 Is Involved in Jasmonic Acid and Ethylene Signaling of Pathogen Response in Arabidopsis PLANT CELL, April 1, 2005; 17(4): 1196 - 1204. [Abstract] [Full Text] [PDF]

				P. Reymond, N. Bodenhausen, R. M.P. Van Poecke, V. Krishnamurthy, M. Dicke, and E. E. Farmer A Conserved Transcript Pattern in Response to a Specialist and a Generalist Herbivore PLANT CELL, November 1, 2004; 16(11): 3132 - 3147. [Abstract] [Full Text] [PDF]

				R. A. Caldo, D. Nettleton, and R. P. Wise Interaction-Dependent Gene Expression in Mla-Specified Response to Barley Powdery Mildew PLANT CELL, September 1, 2004; 16(9): 2514 - 2528. [Abstract] [Full Text] [PDF]

				C. Gachon, A. Mingam, and B. Charrier Real-time PCR: what relevance to plant studies? J. Exp. Bot., July 1, 2004; 55(402): 1445 - 1454. [Abstract] [Full Text] [PDF]

				E. Glawischnig, B. G. Hansen, C. E. Olsen, and B. A. Halkier Camalexin is synthesized from indole-3-acetaldoxime, a key branching point between primary and secondary metabolism in Arabidopsis PNAS, May 25, 2004; 101(21): 8245 - 8250. [Abstract] [Full Text] [PDF]

				J. E. Taylor, P. E. Hatcher, and N. D. Paul Crosstalk between plant responses to pathogens and herbivores: a view from the outside in J. Exp. Bot., January 2, 2004; 55(395): 159 - 168. [Abstract] [Full Text] [PDF]

				F. Katagiri and J. Glazebrook Local Context Finder (LCF) reveals multidimensional relationships among mRNA expression profiles of Arabidopsis responding to pathogen infection PNAS, September 16, 2003; 100(19): 10842 - 10847. [Abstract] [Full Text] [PDF]