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RESEARCH PAPERS ON SYSTEMS BIOLOGY/GENOMICS/BIOINFORMATICS

Comparative Analysis of the Arabidopsis Pollen Transcriptome^1,[w]

Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom (D.H., D.T.); and Institute of Experimental Botany CAS, Rozvojova 135, CZ–165 02 Praha 6, Czech Republic (D.H.)

We present a genome-wide view of the male gametophytic transcriptome in Arabidopsis based on microarray analysis. In comparison with the transcriptome of the sporophyte throughout development, the pollen transcriptome showed reduced complexity and a unique composition. We identified 992 pollen-expressed mRNAs, nearly 40% of which were detected specifically in pollen. Analysis of the functional composition of the pollen transcriptome revealed the over-representation of mRNAs encoding proteins involved in cell wall metabolism, cytoskeleton, and signaling and under-representation of mRNAs involved in transcription and protein synthesis. For several gene families, we observed a common pattern of mutually exclusive gene expression between pollen and sporophytic tissues for different gene family members. Our results provide a 50-fold increase in the knowledge of genes expressed in Arabidopsis pollen. Moreover, we also detail the extensive overlap (61%) of the pollen transcriptome with that of the sporophyte, which provides ample potential to influence sporophytic fitness through gametophytic selection.

¹ This work was supported by a Royal Society/NATO Postdoctoral Fellowship (to D.H.), by the Ministry of Education of the Czech Republic (project no. CE:JI3/98:113100003 to D.H.), and by the Biotechnology and Biological Sciences Research Council under the Investigating Gene Function Initiative (to D.T.).

^[w] The online version of this article contains Web-only data. The supplemental material is available at http://www.plantphysiol.org.

^* Corresponding author; e-mail twe{at}le.ac.uk; fax 44–116–2522791.

Received January 23, 2003; returned for revision February 11, 2003; accepted February 20, 2003.

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