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RESEARCH PAPERS ON SYSTEMS BIOLOGY/GENOMICS/BIOINFORMATICS

The Identification of Candidate Genes for a Reverse Genetic Analysis of Development and Function in the Arabidopsis Gynoecium¹

Reproduction et Développement des Plantes, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon cedex 07, France (C.P.S., M.V.-D., C.F., J.A., T.G., C.D.); and Hitachi Advanced Research Laboratory, Hatoyama, Saitama, 350–0395, Japan (N.K., K.U., M.F.)

The screening for mutants and their subsequent molecular analysis has permitted the identification of a number of genes of Arabidopsis involved in the development and functions of the gynoecium. However, these processes remain far from completely understood. It is clear that in many cases, genetic redundancy and other factors can limit the efficiency of classical mutant screening. We have taken the alternative approach of a reverse genetic analysis of gene function in the Arabidopsis gynoecium. A high-throughput fluorescent differential display screen performed between two Arabidopsis floral homeotic mutants has permitted the identification of a number of genes that are specifically or preferentially expressed in the gynoecium. Here, we present the results of this screen and a detailed characterization of the expression profiles of the genes identified. Our expression analysis makes novel use of several Arabidopsis floral homeotic mutants to provide floral organ-specific gene expression profiles. The results of these studies permit the efficient targeting of effort into a functional analysis of gynoecium-expressed genes.

¹ The laboratory of Reproduction et Développement des Plantes is funded jointly by the Centre National de la Recherche Scientifique, the Institut National de la Recherche Agronomique, the Ecole Normale Supérieure de Lyon and the Université Claude Bernard-Lyon. C.P.S. was funded during this work formerly by a European Community Marie-Curie Fellowship and latterly as a Centre National de la Recherche Scientifique researcher. This work was supported in part by Hitachi (Advanced Research Laboratory grant no. B2023 to M.F.) and by the Program for the Promotion of Basic Research Activities for Innovative Bioscience (grant to M.F.).

^* Corresponding author; e-mail Charlie.Scutt{at}ens-lyon.fr; fax 33–4–72–72–86–00.

Received November 17, 2002; returned for revision January 13, 2003; accepted February 25, 2003.

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