This Article Full Text Full Text (PDF) All Versions of this Article: 132/2/796    most recent pp.102.016089v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (51) Citing Articles via Google Scholar Google Scholar Articles by Vert, G. A. Articles by Curie, C. Search for Related Content PubMed PubMed Citation Articles by Vert, G. A. Articles by Curie, C. Agricola Articles by Vert, G. A. Articles by Curie, C.

ENVIRONMENTAL STRESS AND ADAPTATION

Dual Regulation of the Arabidopsis High-Affinity Root Iron Uptake System by Local and Long-Distance Signals¹

Biochimie et Physiologie Moléculaire des Plantes, Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5004)/Institut National de la Recherche Agronomique/Agro-M/Université Montpellier II, 2 place Viala, F–34060 Montpellier cedex 1, France

Regulation of the root high-affinity iron uptake system by whole-plant signals was investigated at the molecular level in Arabidopsis, through monitoring FRO2 and IRT1 gene expression. These two genes encode the root ferric-chelate reductase and the high-affinity iron transporter, respectively, involved in the iron deficiency-induced uptake system. Recovery from iron-deficient conditions and modulation of apoplastic iron pools indicate that iron itself plays a major role in the regulation of root iron deficiency responses at the mRNA and protein levels. Split-root experiments show that the expression of IRT1 and FRO2 is controlled both by a local induction from the root iron pool and through a systemic pathway involving a shoot-borne signal, both signals being integrated to tightly control production of the root iron uptake proteins. We also show that IRT1 and FRO2 are expressed during the day and down-regulated at night and that this additional control is overruled by iron starvation, indicating that the nutritional status prevails on the diurnal regulation. Our work suggests, for the first time to our knowledge, that like in grasses, the root iron acquisition in strategy I plants may also be under diurnal regulation. On the basis of the new molecular insights provided in this study and given the strict coregulation of IRT1 and FRO2 observed, we present a model of local and long-distance regulation of the root iron uptake system in Arabidopsis.

¹ This work was in part supported by the French Ministry of National Education, Research and Technology (ACI no. 2000–51) and by the Centre National de la Recherche Scientifique (B.D.I. fellowship to G.V.).

^* Corresponding author; e-mail curie{at}ensam.inra.fr; fax 33–4–67–52–57–37.

Received October 11, 2002; returned for revision November 29, 2002; accepted February 4, 2003.

This article has been cited by other articles:

				E. Bacaicoa and J. M. Garcia-Mina Iron Efficiency in Different Cucumber Cultivars: The Importance of Optimizing the Use of Foliar Iron J. Amer. Soc. Hort. Sci., July 1, 2009; 134(4): 405 - 416. [Abstract] [Full Text] [PDF]

				G. Cassin, S. Mari, C. Curie, J.-F. Briat, and P. Czernic Increased sensitivity to iron deficiency in Arabidopsis thaliana overaccumulating nicotianamine J. Exp. Bot., March 1, 2009; 60(4): 1249 - 1259. [Abstract] [Full Text] [PDF]

				L. Kerkeb, I. Mukherjee, I. Chatterjee, B. Lahner, D. E. Salt, and E. L. Connolly Iron-Induced Turnover of the Arabidopsis IRON-REGULATED TRANSPORTER1 Metal Transporter Requires Lysine Residues Plant Physiology, April 1, 2008; 146(4): 1964 - 1973. [Abstract] [Full Text] [PDF]

				L. Lejay, J. Wirth, M. Pervent, J. M.-F. Cross, P. Tillard, and A. Gojon Oxidative Pentose Phosphate Pathway-Dependent Sugar Sensing as a Mechanism for Regulation of Root Ion Transporters by Photosynthesis Plant Physiology, April 1, 2008; 146(4): 2036 - 2053. [Abstract] [Full Text] [PDF]

				S. Santi and W. Schmidt Laser microdissection-assisted analysis of the functional fate of iron deficiency-induced root hairs in cucumber J. Exp. Bot., March 2, 2008; (2008) erm351v1. [Abstract] [Full Text] [PDF]

				M. G. Stacey, A. Patel, W. E. McClain, M. Mathieu, M. Remley, E. E. Rogers, W. Gassmann, D. G. Blevins, and G. Stacey The Arabidopsis AtOPT3 Protein Functions in Metal Homeostasis and Movement of Iron to Developing Seeds Plant Physiology, February 1, 2008; 146(2): 589 - 601. [Abstract] [Full Text] [PDF]

				G. Zocchi, P. De Nisi, M. Dell'Orto, L. Espen, and P. M. Gallina Iron deficiency differently affects metabolic responses in soybean roots J. Exp. Bot., March 1, 2007; 58(5): 993 - 1000. [Abstract] [Full Text] [PDF]

				D. Duy, G. Wanner, A. R. Meda, N. von Wiren, J. Soll, and K. Philippar PIC1, an Ancient Permease in Arabidopsis Chloroplasts, Mediates Iron Transport PLANT CELL, March 1, 2007; 19(3): 986 - 1006. [Abstract] [Full Text] [PDF]

				C. Lucena, B. M. Waters, F. J. Romera, M. J. Garcia, M. Morales, E. Alcantara, and R. Perez-Vicente Ethylene could influence ferric reductase, iron transporter, and H+-ATPase gene expression by affecting FER (or FER-like) gene activity J. Exp. Bot., December 1, 2006; 57(15): 4145 - 4154. [Abstract] [Full Text] [PDF]

				W. F. XU and W. M. SHI Expression Profiling of the 14-3-3 Gene Family in Response to Salt Stress and Potassium and Iron Deficiencies in Young Tomato (Solanum lycopersicum) Roots: Analysis by Real-time RT-PCR Ann. Bot., November 1, 2006; 98(5): 965 - 974. [Abstract] [Full Text] [PDF]

				G. Schaaf, A. Honsbein, A. R. Meda, S. Kirchner, D. Wipf, and N. von Wiren AtIREG2 Encodes a Tonoplast Transport Protein Involved in Iron-dependent Nickel Detoxification in Arabidopsis thaliana Roots J. Biol. Chem., September 1, 2006; 281(35): 25532 - 25540. [Abstract] [Full Text] [PDF]

				M. A. Martinez-Cordero, V. Martinez, and F. Rubio High-affinity K+ uptake in pepper plants J. Exp. Bot., June 1, 2005; 56(416): 1553 - 1562. [Abstract] [Full Text] [PDF]

				G. Schaaf, A. Schikora, J. Haberle, G. Vert, U. Ludewig, J.-F. Briat, C. Curie, and N. von Wiren A Putative Function for the Arabidopsis Fe-Phytosiderophore Transporter Homolog AtYSL2 in Fe and Zn Homeostasis Plant Cell Physiol., May 1, 2005; 46(5): 762 - 774. [Abstract] [Full Text] [PDF]

				E. P. Colangelo and M. L. Guerinot The Essential Basic Helix-Loop-Helix Protein FIT1 Is Required for the Iron Deficiency Response PLANT CELL, December 1, 2004; 16(12): 3400 - 3412. [Abstract] [Full Text] [PDF]

				M. Menckhoff and S. Luthje Transmembrane electron transport in sealed and NAD(P)H-loaded right-side-out plasma membrane vesicles isolated from maize (Zea mays L.) roots J. Exp. Bot., June 1, 2004; 55(401): 1343 - 1349. [Abstract] [Full Text] [PDF]