First published online May 1, 2003; 10.1104/pp.103.020800
Plant Physiology 132:861-869 (2003)
© 2003 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
AtCHIP, a U-Box-Containing E3 Ubiquitin Ligase, Plays a Critical Role in Temperature Stress Tolerance in Arabidopsis1
Juqiang Yan,
Jing Wang,
Qingtian Li2,
Jae Ryoung Hwang,
Cam Patterson and
Hong Zhang*
Department of Biological Sciences, Texas Tech University, Lubbock, Texas 79409 (J.Y., J.W., Q.L., H.Z.); Department of Cell and Developmental Biology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599–7090 (J.R.H., C.P.); and Program in Molecular Cardiology and the Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599–7075 (J.R.H., C.P.)
The Arabidopsis gene AtCHIP encodes a protein with three tetratricopeptide repeats and a U-box domain, which is structurally similar to the animal CHIP proteins, a new class of E3 ubiquitin ligases. Like animal CHIP proteins, AtCHIP has E3 ubiquitin ligase activity in vitro. AtCHIP is a single-copy gene, and its transcript is up-regulated by several stress conditions such as low and high temperatures. However, increased AtCHIP expression alone was not correlated with increased stress tolerance; in fact, overexpression of AtCHIP in Arabidopsis rendered plants more sensitive to both low- and high-temperature treatments. Higher electrolyte leakage was observed in leaves of AtCHIP overexpression plants after chilling temperature treatment, suggesting that membrane function is likely impaired in these plants under such a condition. These results indicate that AtCHIP plays an important role in plant cellular metabolism under temperature stress conditions.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.020800.
1 This work was supported by grants from the Texas Advanced Technology Program.
2 Present address: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030.
* Corresponding author; e-mail brahz{at}ttacs.ttu.edu; fax 806-742-2963.
Received January 21, 2003;
returned for revision March 4, 2003;
accepted March 24, 2003.
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