First published online May 15, 2003; 10.1104/pp.103.021261
Plant Physiology 132:893-906 (2003)
© 2003 American Society of Plant Biologists
ENVIRONMENTAL STRESS AND ADAPTATION
Gene Expression Phenotypes of Arabidopsis Associated with Sensitivity to Low Temperatures[w]
Nicholas J. Provart1,2,
Pedro Gil1,
Wenqiong Chen3,
Bin Han3,
Hur-Song Chang3,
Xun Wang4 and
Tong Zhu4,*
Torrey Mesa Research Institute, Syngenta Research and Technology, 3115 Merryfield Row, San Diego, California 92121
Chilling is a common abiotic stress that leads to economic losses in agriculture. By comparing the transcriptome of Arabidopsis under normal (22°C) and chilling (13°C) conditions, we have surveyed the molecular responses of a chilling-resistant plant to acclimate to a moderate reduction in temperature. The mRNA accumulation of approximately 20% of the approximately 8,000 genes analyzed was affected by chilling. In particular, a highly significant number of genes involved in protein biosynthesis displayed an increase in transcript abundance. We have analyzed the molecular phenotypes of 12 chilling-sensitive mutants exposed to 13°C before any visible phenotype could be detected. The number and pattern of expression of chilling-responsive genes in the mutants were consistent with their final degree of chilling injury. The mRNA accumulation profiles for the chilling-lethal mutants chs1, chs2, and chs3 were highly similar and included extensive chilling-induced and mutant-specific alterations in gene expression. The expression pattern of the mutants upon chilling suggests that the normal function of the mutated loci prevents a damaging widespread effect of chilling on transcriptional regulation. In addition, we have identified 634 chilling-responsive genes with aberrant expression in all of the chilling-lethal mutants. This reference gene list, including genes related to lipid metabolism, chloroplast function, carbohydrate metabolism and free radical detoxification, represents a potential source for genes with a critical role in plant acclimation to suboptimal temperatures. The comparison of transcriptome profiles after transfer of Arabidopsis plants from 22°C to 13°C versus transfer to 4°C suggests that quantitative and temporal differences exist between these molecular responses.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.021261.
[w] The online version of this article contains Web-only data. The supplemental material is available at http://www.plantphysiol.org.
1 These authors contributed equally to the paper
2 Present address: Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, ON, Canada M5S 3B2.
3 Present address: Diversa Corporation, 4955 Directors Place, San Diego, CA 92121.
4 Present address: Syngenta Biotechnology Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709.
* Corresponding author;e-mail tong.zhu{at}syngenta.com;fax 919–541–8585.
Received January 3, 2003;
returned for revision March 2, 2003;
accepted March 25, 2003.
This article has been cited by other articles:
|
|
|
|
 
M. Rogalski, M. A. Schottler, W. Thiele, W. X. Schulze, and R. Bock
Rpl33, a Nonessential Plastid-Encoded Ribosomal Protein in Tobacco, Is Required under Cold Stress Conditions
PLANT CELL,
August 1, 2008;
20(8):
2221 - 2237.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhu, J. C. Jeong, Y. Zhu, I. Sokolchik, S. Miyazaki, J.-K. Zhu, P. M. Hasegawa, H. J. Bohnert, H. Shi, D.-J. Yun, et al.
Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance
PNAS,
March 25, 2008;
105(12):
4945 - 4950.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Alferez, G. Y. Zhong, and J. K. Burns
A citrus abscission agent induces anoxia- and senescence-related gene expression in Arabidopsis
J. Exp. Bot.,
July 1, 2007;
58(10):
2451 - 2462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Herman, K. Rotter, R. Premakumar, G Elwinger, R. Bae, L. Ehler-King, S. Chen, and D. P. Livingston III
Additional freeze hardiness in wheat acquired by exposure to -3 {degrees}C is associated with extensive physiological, morphological, and molecular changes
J. Exp. Bot.,
November 1, 2006;
57(14):
3601 - 3618.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hewezi, M. Leger, W. El Kayal, and L. Gentzbittel
Transcriptional profiling of sunflower plants growing under low temperatures reveals an extensive down-regulation of gene expression associated with chilling sensitivity
J. Exp. Bot.,
September 1, 2006;
57(12):
3109 - 3122.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Hannah, D. Wiese, S. Freund, O. Fiehn, A. G. Heyer, and D. K. Hincha
Natural Genetic Variation of Freezing Tolerance in Arabidopsis
Plant Physiology,
September 1, 2006;
142(1):
98 - 112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. T. Svensson, C. Crosatti, C. Campoli, R. Bassi, A. M. Stanca, T. J. Close, and L. Cattivelli
Transcriptome Analysis of Cold Acclimation in Barley Albina and Xantha Mutants
Plant Physiology,
May 1, 2006;
141(1):
257 - 270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. V. Krutovsky and D. B. Neale
Nucleotide Diversity and Linkage Disequilibrium in Cold-Hardiness- and Wood Quality-Related Candidate Genes in Douglas Fir
Genetics,
December 1, 2005;
171(4):
2029 - 2041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. AGARWAL and A. GROVER
Isolation and Transcription Profiling of Low-O2 Stress-Associated cDNA Clones from the Flooding-stress-tolerant FR13A Rice Genotype
Ann. Bot.,
October 1, 2005;
96(5):
831 - 844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Dong, S. T. Kim, and E. M. Lord
Plantacyanin Plays a Role in Reproduction in Arabidopsis
Plant Physiology,
June 1, 2005;
138(2):
778 - 789.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Bruno and C. M. Wetzel
The early light-inducible protein (ELIP) gene is expressed during the chloroplast-to-chromoplast transition in ripening tomato fruit
J. Exp. Bot.,
December 1, 2004;
55(408):
2541 - 2548.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yamauchi, M. Ogawa, A. Kuwahara, A. Hanada, Y. Kamiya, and S. Yamaguchi
Activation of Gibberellin Biosynthesis and Response Pathways by Low Temperature during Imbibition of Arabidopsis thaliana Seeds
PLANT CELL,
February 1, 2004;
16(2):
367 - 378.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
