Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online May 15, 2003; 10.1104/pp.103.021261

Plant Physiology 132:893-906 (2003)
© 2003 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
132/2/893    most recent
pp.103.021261v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (39)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Provart, N. J.
Right arrow Articles by Zhu, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Provart, N. J.
Right arrow Articles by Zhu, T.
Agricola
Right arrow Articles by Provart, N. J.
Right arrow Articles by Zhu, T.
ENVIRONMENTAL STRESS AND ADAPTATION

Gene Expression Phenotypes of Arabidopsis Associated with Sensitivity to Low Temperatures[w]

Nicholas J. Provart1,2, Pedro Gil1, Wenqiong Chen3, Bin Han3, Hur-Song Chang3, Xun Wang4 and Tong Zhu4,*

Torrey Mesa Research Institute, Syngenta Research and Technology, 3115 Merryfield Row, San Diego, California 92121

Chilling is a common abiotic stress that leads to economic losses in agriculture. By comparing the transcriptome of Arabidopsis under normal (22°C) and chilling (13°C) conditions, we have surveyed the molecular responses of a chilling-resistant plant to acclimate to a moderate reduction in temperature. The mRNA accumulation of approximately 20% of the approximately 8,000 genes analyzed was affected by chilling. In particular, a highly significant number of genes involved in protein biosynthesis displayed an increase in transcript abundance. We have analyzed the molecular phenotypes of 12 chilling-sensitive mutants exposed to 13°C before any visible phenotype could be detected. The number and pattern of expression of chilling-responsive genes in the mutants were consistent with their final degree of chilling injury. The mRNA accumulation profiles for the chilling-lethal mutants chs1, chs2, and chs3 were highly similar and included extensive chilling-induced and mutant-specific alterations in gene expression. The expression pattern of the mutants upon chilling suggests that the normal function of the mutated loci prevents a damaging widespread effect of chilling on transcriptional regulation. In addition, we have identified 634 chilling-responsive genes with aberrant expression in all of the chilling-lethal mutants. This reference gene list, including genes related to lipid metabolism, chloroplast function, carbohydrate metabolism and free radical detoxification, represents a potential source for genes with a critical role in plant acclimation to suboptimal temperatures. The comparison of transcriptome profiles after transfer of Arabidopsis plants from 22°C to 13°C versus transfer to 4°C suggests that quantitative and temporal differences exist between these molecular responses.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.021261.

[w] The online version of this article contains Web-only data. The supplemental material is available at http://www.plantphysiol.org.

1 These authors contributed equally to the paper

2 Present address: Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, ON, Canada M5S 3B2.

3 Present address: Diversa Corporation, 4955 Directors Place, San Diego, CA 92121.

4 Present address: Syngenta Biotechnology Inc., 3054 Cornwallis Road, Research Triangle Park, NC 27709.

* Corresponding author;e-mail tong.zhu{at}syngenta.com;fax 919–541–8585.

Received January 3, 2003; returned for revision March 2, 2003; accepted March 25, 2003.




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Zhu, J. C. Jeong, Y. Zhu, I. Sokolchik, S. Miyazaki, J.-K. Zhu, P. M. Hasegawa, H. J. Bohnert, H. Shi, D.-J. Yun, et al.
Involvement of Arabidopsis HOS15 in histone deacetylation and cold tolerance
PNAS, March 25, 2008; 105(12): 4945 - 4950.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Alferez, G. Y. Zhong, and J. K. Burns
A citrus abscission agent induces anoxia- and senescence-related gene expression in Arabidopsis
J. Exp. Bot., July 1, 2007; 58(10): 2451 - 2462.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. M. Herman, K. Rotter, R. Premakumar, G Elwinger, R. Bae, L. Ehler-King, S. Chen, and D. P. Livingston III
Additional freeze hardiness in wheat acquired by exposure to -3 {degrees}C is associated with extensive physiological, morphological, and molecular changes
J. Exp. Bot., November 1, 2006; 57(14): 3601 - 3618.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Hewezi, M. Leger, W. El Kayal, and L. Gentzbittel
Transcriptional profiling of sunflower plants growing under low temperatures reveals an extensive down-regulation of gene expression associated with chilling sensitivity
J. Exp. Bot., September 1, 2006; 57(12): 3109 - 3122.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Hannah, D. Wiese, S. Freund, O. Fiehn, A. G. Heyer, and D. K. Hincha
Natural Genetic Variation of Freezing Tolerance in Arabidopsis
Plant Physiology, September 1, 2006; 142(1): 98 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. T. Svensson, C. Crosatti, C. Campoli, R. Bassi, A. M. Stanca, T. J. Close, and L. Cattivelli
Transcriptome Analysis of Cold Acclimation in Barley Albina and Xantha Mutants
Plant Physiology, May 1, 2006; 141(1): 257 - 270.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. V. Krutovsky and D. B. Neale
Nucleotide Diversity and Linkage Disequilibrium in Cold-Hardiness- and Wood Quality-Related Candidate Genes in Douglas Fir
Genetics, December 1, 2005; 171(4): 2029 - 2041.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
S. AGARWAL and A. GROVER
Isolation and Transcription Profiling of Low-O2 Stress-Associated cDNA Clones from the Flooding-stress-tolerant FR13A Rice Genotype
Ann. Bot., October 1, 2005; 96(5): 831 - 844.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Dong, S. T. Kim, and E. M. Lord
Plantacyanin Plays a Role in Reproduction in Arabidopsis
Plant Physiology, June 1, 2005; 138(2): 778 - 789.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. K. Bruno and C. M. Wetzel
The early light-inducible protein (ELIP) gene is expressed during the chloroplast-to-chromoplast transition in ripening tomato fruit
J. Exp. Bot., December 1, 2004; 55(408): 2541 - 2548.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Yamauchi, M. Ogawa, A. Kuwahara, A. Hanada, Y. Kamiya, and S. Yamaguchi
Activation of Gibberellin Biosynthesis and Response Pathways by Low Temperature during Imbibition of Arabidopsis thaliana Seeds
PLANT CELL, February 1, 2004; 16(2): 367 - 378.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists