Plant Physiology 132:1391-1404 (2003)
© 2003 American Society of Plant Biologists
DEVELOPMENT AND HORMONE ACTION
Interacting Transcription Factors from the Three-Amino Acid Loop Extension Superclass Regulate Tuber Formation1
Hao Chen,
Faye M. Rosin,
Salomé Prat and
David J. Hannapel*
Interdepartmental Plant Physiology Major (H.C., D.J.H.) and Molecular,
Cellular, and Developmental Biology Major (F.M.R., D.J.H.), Department of
Horticulture, Iowa State University, Ames, Iowa 50011–1100; and
Department of Plant Molecular Genetics, National Center of Biotechnology,
Consejo Superior de Investigaciones Científicas, Cantoblanco Campus
University of Madrid, Madrid, Spain (S.P.)
Using the yeast (Saccharomyces cerevisiae) two-hybrid system and a
potato (Solanum tuberosum) KNOX protein, designated POTH1, as bait,
we have identified seven distinct interacting proteins from a stolon library
of potato. All seven cDNAs are members of the BEL1-like family of
transcription factors. Among these proteins, there are at least four regions
of high sequence conservation including the homeodomain, the
proline-tyrosine-proline three-amino acid loop extension, the SKY box, and a
120-amino acid region upstream from the homeodomain. Through deletion
analysis, we identified a protein-binding domain present in the carboxy end of
the KNOX domain of POTH1. The protein-binding domain in the BEL1 protein is
located in the amino-terminal one-half of the 120-residue conserved region of
the BELs. RNA-blot analysis showed differential patterns of RNA accumulation
for the BELs in various potato organs. The level of StBEL5 mRNA
increased in response to a short-day photoperiod in both leaves and stolons.
Similar to sense mutants of POTH1, transgenic lines that
overexpressed StBEL5 exhibited enhanced tuber formation even under
noninductive conditions. Unlike POTH1 sense lines, however, these BEL
lines did not exhibit the extreme leaf and stem morphology characteristic of
KNOX overexpressers and displayed a more rapid rate of growth than control
plants. Both StBEL5 and POTH1 sense lines exhibited an
increase in cytokinin levels in shoot tips. StBEL5 lines also
exhibited a decrease in the levels of GA 20-oxidase1 mRNA in stolon tips from
long-day plants. Our results demonstrate an interaction between KNOX and
BEL1-like transcription factors of potato that may potentially regulate
processes of development.
Article, publication date, and citation information can be found at
http://www.plantphysiol.org/cgi/doi/10.1104/pp.103.022434.
1 This work was supported in part by the Iowa Agriculture and Home Economics
Experiment Station. This is journal paper no. J–19520 of the Iowa
Agriculture and Home Economics Experiment Station (Ames, IA; project no.
3701).
*
Corresponding author; e-mail
djh{at}iastate.edu;
fax 515–294–0730.
Received February 20, 2003;
returned for revision March 28, 2003;
accepted April 4, 2003.
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