This Article Full Text Full Text (PDF) All Versions of this Article: 132/3/1405    most recent pp.103.021808v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Plant Physiol. Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (23) Citing Articles via Google Scholar Google Scholar Articles by Liu, Z. Articles by Hall, J. D. Search for Related Content PubMed PubMed Citation Articles by Liu, Z. Articles by Hall, J. D. Agricola Articles by Liu, Z. Articles by Hall, J. D.

ENVIRONMENTAL STRESS AND ADAPTATION

Arabidopsis UVH6, a Homolog of Human XPD and Yeast RAD3 DNA Repair Genes, Functions in DNA Repair and Is Essential for Plant Growth¹

Departments of Molecular and Cellular Biology (Z.L., D.W.M., J.D.H.) and Biochemistry and Molecular Biophysics (S.-W.H., M.E., E.V., D.W.M., J.D.H.), University of Arizona, Tucson, Arizona 85721; and Department of Carcinogenesis, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030 (D.L.M.)

To evaluate the genetic control of stress responses in Arabidopsis, we have analyzed a mutant (uvh6-1) that exhibits increased sensitivity to UV light, a yellow-green leaf coloration, and mild growth defects. We have mapped the uvh6-1 locus to chromosome I and have identified a candidate gene, AtXPD, within the corresponding region. This gene shows sequence similarity to the human (Homo sapiens) XPD and yeast (Saccharomyces cerevisiae) RAD3 genes required for nucleotide excision repair. We propose that UVH6 is equivalent to AtXPD because uvh6-1 mutants carry a mutation in a conserved residue of AtXPD and because transformation of uvh6-1 mutants with wild-type AtXPD DNA suppresses both UV sensitivity and other defective phenotypes. Furthermore, the UVH6/AtXPD protein appears to play a role in repair of UV photoproducts because the uvh6-1 mutant exhibits a moderate defect in the excision of UV photoproducts. This defect is also suppressed by transformation with UVH6/AtXPD DNA. We have further identified a T-DNA insertion in the UVH6/AtXPD gene (uvh6-2). Plants carrying homozygous insertions were not detected in analyses of progeny from plants heterozygous for the insertion. Thus, homozygous insertions appear to be lethal. We conclude that the UVH6/AtXPD gene is required for UV resistance and is an essential gene in Arabidopsis.

¹ This work was supported by the National Science Foundation (grant no. MCB–9728125 to D.W.M.) and by the U.S. Department of Agriculture National Research Initiative Competitive Grants Program (grant no. 99–3515100 to E.V.).

² Present address: Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430.

³ Present address: Department of Applied Plant Sciences, Chonnam National University, 300 Yang-Bong Dong, Buk-Gu, Gwang-ju 500–757, South Korea.

^* Corresponding author; e-mail jdhall{at}u.arizona.edu; fax 520 621–3709.

Received February 8, 2003; returned for revision March 30, 2003; accepted April 17, 2003.

Related articles in Plant Physiol.:

Peter V. Minorsky
Plant Physiol. 2003 132: 1117-1118. [Full Text]  

This article has been cited by other articles:

				R. A. Pugh, M. Honda, H. Leesley, A. Thomas, Y. Lin, M. J. Nilges, I. K. O. Cann, and M. Spies The Iron-containing Domain Is Essential in Rad3 Helicases for Coupling of ATP Hydrolysis to DNA Translocation and for Targeting the Helicase to the Single-stranded DNA-Double-stranded DNA Junction J. Biol. Chem., January 18, 2008; 283(3): 1732 - 1743. [Abstract] [Full Text] [PDF]

				S. Takahashi, A. N. Sakamoto, A. Tanaka, and K. Shimizu AtREV1, a Y-Family DNA Polymerase in Arabidopsis, Has Deoxynucleotidyl Transferase Activity in Vitro Plant Physiology, November 1, 2007; 145(3): 1052 - 1060. [Abstract] [Full Text] [PDF]

				J. HIDEMA and T. KUMAGAI Sensitivity of Rice to Ultraviolet-B Radiation Ann. Bot., June 1, 2006; 97(6): 933 - 942. [Abstract] [Full Text] [PDF]

				T. Ueda, T. Sato, J. Hidema, T. Hirouchi, K. Yamamoto, T. Kumagai, and M. Yano qUVR-10, a Major Quantitative Trait Locus for Ultraviolet-B Resistance in Rice, Encodes Cyclobutane Pyrimidine Dimer Photolyase Genetics, December 1, 2005; 171(4): 1941 - 1950. [Abstract] [Full Text] [PDF]

				J. Larkindale, J. D. Hall, M. R. Knight, and E. Vierling Heat Stress Phenotypes of Arabidopsis Mutants Implicate Multiple Signaling Pathways in the Acquisition of Thermotolerance Plant Physiology, June 1, 2005; 138(2): 882 - 897. [Abstract] [Full Text] [PDF]

				S. Kimura, Y. Tahira, T. Ishibashi, Y. Mori, T. Mori, J. Hashimoto, and K. Sakaguchi DNA repair in higher plants; photoreactivation is the major DNA repair pathway in non-proliferating cells while excision repair (nucleotide excision repair and base excision repair) is active in proliferating cells Nucleic Acids Res., May 18, 2004; 32(9): 2760 - 2767. [Abstract] [Full Text] [PDF]