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ENVIRONMENTAL STRESS AND ADAPTATION

TaVRT-1, a Putative Transcription Factor Associated with Vegetative to Reproductive Transition in Cereals¹

Département des Sciences Biologiques, Université du Québec à Montréal, Case Postale 8888, Succursale Centre-ville, Montréal, Québec, Canada H3C 3P8 (J.D., N.A.K., G.B., F.S.); and Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5A8 (A.E.L., D.B.F.)

The molecular genetics of vernalization, defined as the promotion of flowering by cold treatment, is still poorly understood in cereals. To better understand this mechanism, we cloned and characterized a gene that we named TaVRT-1 (wheat [Triticum aestivum] vegetative to reproductive transition-1). Molecular and sequence analyses indicated that this gene encodes a protein homologous to the MADS-box family of transcription factors that comprises certain flowering control proteins in Arabidopsis. Mapping studies have localized this gene to the Vrn-1 regions on the long arms of homeologous group 5 chromosomes, regions that are associated with vernalization and freezing tolerance (FT) in wheat. The level of expression of TaVRT-1 is positively associated with the vernalization response and transition from vegetative to reproductive phase and is negatively associated with the accumulation of COR genes and degree of FT. Comparisons among different wheat genotypes, near-isogenic lines, and cereal species, which differ in their vernalization response and FT, indicated that the gene is inducible only in those species that require vernalization, whereas it is constitutively expressed in spring habit genotypes. In addition, experiments using both the photoperiod-sensitive barley (Hordeum vulgare cv Dicktoo) and short or long day de-acclimated wheat revealed that the expression of TaVRT-1 is also regulated by photoperiod. These expression studies indicate that photoperiod and vernalization may regulate this gene through separate pathways. We suggest that TaVRT-1 is a key developmental gene in the regulatory pathway that controls the transition from the vegetative to reproductive phase in cereals.

Note Added in Proof

After acceptance of this report, TaVRT-1 was mapped to a 0.03-centimorgan interval that was completely linked to the VRN1 locus in Triticum monococcum [Yan L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J (2003) Positional cloning of the wheat vernalization gene VRNI. PNAS 100: 6263–6268]. In this study, three independent deletions were found in its promoter region in spring genotypes, suggesting that it may be the VRN1 gene.

¹ This work was supported by the Natural Sciences and Engineering Research Council of Canada (research grants to F.S. and D.B.F.), by Genome Canada, by Génome Québec, and by Genome Prairie.

² These authors contributed equally to this paper.

^* Corresponding author; e-mail sarhan.fathey{at}uqam.ca; fax 514–987–4647.

Received March 13, 2003; returned for revision April 14, 2003; accepted April 23, 2003.

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