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Plant Physiology 132:1870-1883 (2003)
© 2003 American Society of Plant Biologists

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PLANTS INTERACTING WITH OTHER ORGANISMS

MPB2C, a Microtubule-Associated Plant Protein Binds to and Interferes with Cell-to-Cell Transport of Tobacco Mosaic Virus Movement Protein1

Friedrich Kragler2,3, Mirela Curin3, Kateryna Trutnyeva, Andreas Gansch and Elisabeth Waigmann*

Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Institute of Medical Biochemistry, University of Vienna, Dr. Bohr-Gasse. 9, A-1030 Vienna, Austria

The movement protein of tobacco mosaic virus, MP30, mediates viral cell-to-cell transport via plasmodesmata. The complex MP30 intra- and intercellular distribution pattern includes localization to the endoplasmic reticulum, cytoplasmic bodies, microtubules, and plasmodesmata and likely requires interaction with plant endogenous factors. We have identified and analyzed an MP30-interacting protein, MPB2C, from the host plant Nicotiana tabacum. MPB2C constitutes a previously uncharacterized microtubule-associated protein that binds to and colocalizes with MP30 at microtubules. In vivo studies indicate that MPB2C mediates accumulation of MP30 at microtubules and interferes with MP30 cell-to-cell movement. In contrast, intercellular transport of a functionally enhanced MP30 mutant, which does not accumulate and colocalize with MP30 at microtubules, is not impaired by MPB2C. Together, these data support the concept that MPB2C is not required for MP30 cell-to-cell movement but may act as a negative effector of MP30 cell-to-cell transport activity.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.022269.

1 This work was supported by the Austrian Science Foundation (grant nos. P12614–MOB and Sfb17, project part 08 to E.W.). E.W. was supported by an Austrian Programme for Advanced Research and Technology fellowship (APART 441) from the Austrian Academy of Science.

2 Present address: Section of Plant Science, University of California Davis, One Shields Ave, Davis, CA 95616.

3 These authors contributed equally to this paper.

* Corresponding author; e-mail waigmann{at}bch.univie.ac.at; fax 43–1–4277–9616.

Received March 5, 2003; returned for revision March 21, 2003; accepted April 24, 2003.




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