Plant Physiology 132:1973-1981 (2003)
© 2003 American Society of Plant Biologists
PLANTS INTERACTING WITH OTHER ORGANISMS
Induction of Hypersensitive Cell Death by Hydrogen Peroxide Produced through Polyamine Degradation in Tobacco Plants1
Hiroshi Yoda,
Yube Yamaguchi and
Hiroshi Sano*
Research and Education Center for Genetic Information, Nara Institute of
Science and Technology, Nara 630–0192, Japan
Screening immediate-early responding genes during the hypersensitive
response (HR) against tobacco mosaic virus infection in tobacco (Nicotiana
tabacum) plants, we identified a gene encoding ornithine decarboxylase.
Subsequent analyses showed that other genes involved in polyamine biosynthesis
were also up-regulated, resulting in the accumulation of polyamines in
apoplasts of tobacco mosaic virus-infected leaves. Inhibitors of polyamine
biosynthesis,  -difluoromethyl-ornithine, however, suppressed
accumulation of polyamines, and the rate of HR was reduced. In contrast,
polyamine infiltration into a healthy leaf induced the generation of hydrogen
peroxide and simultaneously caused HR-like cell death. Polyamine oxidase
activity in the apoplast increased up to 3-fold that of the basal level during
the HR, and its suppression with a specific inhibitor, guazatine, resulted in
reduced HR. Because it is established that hydrogen peroxide is one of the
degradation products of polyamines, these results indicate that one of the
biochemical events in the HR is production of polyamines, whose degradation
induces hydrogen peroxide, eventually resulting in hypersensitive cell
death.
Article, publication date, and citation information can be found at
www.plantphysiol.org/cgi/doi/10.1104/pp.103.024737.
1 This work was supported by the Research for the Future Program of the Japan
Society for the Promotion of Science (grant no. JSPS-RFTF00L01604).
*
Corresponding author; e-mail
sano{at}gtc.aist-nara.ac.jp;
fax 81–743–72–5659.
Received April 2, 2003;
returned for revision April 24, 2003;
accepted May 9, 2003.
This article has been cited by other articles:
|
|
|
|
 
A. A. Rodriguez, S. J. Maiale, A. B. Menendez, and O. A. Ruiz
Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
J. Exp. Bot.,
November 1, 2009;
60(15):
4249 - 4262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. N. Moschou, P. F. Sarris, N. Skandalis, A. H. Andriopoulou, K. A. Paschalidis, N. J. Panopoulos, and K. A. Roubelakis-Angelakis
Engineered Polyamine Catabolism Preinduces Tolerance of Tobacco to Bacteria and Oomycetes
Plant Physiology,
April 1, 2009;
149(4):
1970 - 1981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Marina, S. J. Maiale, F. R. Rossi, M. F. Romero, E. I. Rivas, A. Garriz, O. A. Ruiz, and F. L. Pieckenstain
Apoplastic Polyamine Oxidation Plays Different Roles in Local Responses of Tobacco to Infection by the Necrotrophic Fungus Sclerotinia sclerotiorum and the Biotrophic Bacterium Pseudomonas viridiflava
Plant Physiology,
August 1, 2008;
147(4):
2164 - 2178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. N. Moschou, M. Sanmartin, A. H. Andriopoulou, E. Rojo, J. J. Sanchez-Serrano, and K. A. Roubelakis-Angelakis
Bridging the Gap between Plant and Mammalian Polyamine Catabolism: A Novel Peroxisomal Polyamine Oxidase Responsible for a Full Back-Conversion Pathway in Arabidopsis
Plant Physiology,
August 1, 2008;
147(4):
1845 - 1857.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. N. Moschou, K. A. Paschalidis, I. D. Delis, A. H. Andriopoulou, G. D. Lagiotis, D. I. Yakoumakis, and K. A. Roubelakis-Angelakis
Spermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in Tobacco
PLANT CELL,
June 1, 2008;
20(6):
1708 - 1724.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. An, W. Jing, Y. Liu, and W. Zhang
Hydrogen peroxide generated by copper amine oxidase is involved in abscisic acid-induced stomatal closure in Vicia faba
J. Exp. Bot.,
March 1, 2008;
59(4):
815 - 825.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Angelini, A. Tisi, G. Rea, M. M. Chen, M. Botta, R. Federico, and A. Cona
Involvement of Polyamine Oxidase in Wound Healing
Plant Physiology,
January 1, 2008;
146(1):
162 - 177.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yoda, Y. Hiroi, and H. Sano
Polyamine Oxidase Is One of the Key Elements for Oxidative Burst to Induce Programmed Cell Death in Tobacco Cultured Cells
Plant Physiology,
September 1, 2006;
142(1):
193 - 206.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Tavladoraki, M. N. Rossi, G. Saccuti, M. A. Perez-Amador, F. Polticelli, R. Angelini, and R. Federico
Heterologous Expression and Biochemical Characterization of a Polyamine Oxidase from Arabidopsis Involved in Polyamine Back Conversion
Plant Physiology,
August 1, 2006;
141(4):
1519 - 1532.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Cona, G. Rea, M. Botta, F. Corelli, R. Federico, and R. Angelini
Flavin-containing polyamine oxidase is a hydrogen peroxide source in the oxidative response to the protein phosphatase inhibitor cantharidin in Zea mays L.
J. Exp. Bot.,
July 1, 2006;
57(10):
2277 - 2289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mateo, P. Muhlenbock, C. Rusterucci, C. C.-C. Chang, Z. Miszalski, B. Karpinska, J. E. Parker, P. M. Mullineaux, and S. Karpinski
LESION SIMULATING DISEASE 1 Is Required for Acclimation to Conditions That Promote Excess Excitation Energy
Plant Physiology,
September 1, 2004;
136(1):
2818 - 2830.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Rea, M. C. de Pinto, R. Tavazza, S. Biondi, V. Gobbi, P. Ferrante, L. De Gara, R. Federico, R. Angelini, and P. Tavladoraki
Ectopic Expression of Maize Polyamine Oxidase and Pea Copper Amine Oxidase in the Cell Wall of Tobacco Plants
Plant Physiology,
April 1, 2004;
134(4):
1414 - 1426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
