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Plant Physiology 132:2135-2143 (2003)
© 2003 American Society of Plant Biologists

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DEVELOPMENT AND HORMONE ACTION

PAUSED Encodes the Arabidopsis Exportin-t Ortholog1

Christine A. Hunter, Milo J. Aukerman2, Hui Sun, Maria Fokina and R. Scott Poethig*

Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104–6018

Los1p/exportin-t (XPOT) mediates the nuclear export of tRNAs in yeast and mammals. The requirements for this transport pathway are unclear, however, because los1 mutations do not affect yeast growth, and the phenotype of XPOT mutations in mammals is unknown. Here, we show that PAUSED (PSD) is the Arabidopsis ortholog of LOS1/XPOT and is capable of rescuing the tRNA export defect of los1 in Brewer's yeast (Saccharomyces cerevisiae), suggesting that its function has been conserved. Putative null alleles of PSD disrupt the initiation of the shoot apical meristem and delay leaf initiation after germination, the emergence of the radicle and lateral roots, and the transition to flowering. Plants doubly mutant for psd and hasty, the Arabidopsis ortholog of exportin 5, are viable but have a more severe phenotype than either single mutant. These results suggest that PSD plays a role in tRNA export in Arabidopsis, but that at least one—and perhaps several—additional tRNA export pathways also exist. The PSD transcript is broadly expressed during development and is alternatively spliced in the 3'-untranslated region. No temporal or spatial difference in the abundance of different splice forms was observed. We propose that the mutant phenotype of psd reflects defects in developmental events and cell/tissue types that require elevated levels of protein synthesis and are therefore acutely sensitive to a reduction in tRNA export.

1 This work was supported by the National Institutes of Health (grants to C.A.H. and R.S.P.).

2 Present address: DuPont Ag Biotech, Delaware Technology Park, Suite 200, 1 Innovation Way, Newark, DE 19711.

* Corresponding author; e-mail spoethig{at}sas.upenn.edu; fax 215–898–8780.

Received March 10, 2003; returned for revision April 6, 2003; accepted May 6, 2003.


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