This Article Full Text Full Text (PDF) All Versions of this Article: 133/1/161    most recent pp.103.023457v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (39) Citing Articles via Google Scholar Google Scholar Articles by Hoa, T. T. C. Articles by Beyer, P. Search for Related Content PubMed PubMed Citation Articles by Hoa, T. T. C. Articles by Beyer, P. Agricola Articles by Hoa, T. T. C. Articles by Beyer, P.

BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Golden Indica and Japonica Rice Lines Amenable to Deregulation¹

Institute for Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland (T.T.C.H., I.P.); and University of Freiburg, Center for Applied Biosciences, Schänzlestrasse 1, D-79104 Freiburg, Germany (S.A.-B., P.S., P.B.)

As an important step toward free access and, thus, impact of GoldenRice, a freedom-to-operate situation has been achieved for developing countries for the technology involved. Specifically, to carry the invention beyond its initial "proof-of-concept" status in a Japonica rice (Oryza sativa) cultivar, we report here on two transformed elite Indica varieties (IR64 and MTL250) plus one Japonica variety Taipei 309. Indica varieties are predominantly consumed in the areas with vitamin A deficiency. To conform with regulatory constraints, we changed the vector backbone, investigated the absence of beyond-border transfer, and relied on Agrobacterium tumefaciens-mediated transformation to obtain defined integration patterns. To avoid an antibiotic selection system, we now rely exclusively on phosphomannose isomerase as the selectable marker. Single integrations were given a preference to minimize potential epigenetic effects in subsequent generations. These novel lines, now in the T₃ generation, are highly valuable because they are expected to more readily receive approval for follow-up studies such as nutritional and risk assessments and for breeding approaches leading to locally adapted variety development.

² Present address: Cuu Long Delta Rice Research Institute, Omon, Cantho, Vietnam.

^* Corresponding author; e-mail peter.beyer{at}biologie.unifreiburg.de; fax 49-761-203-2675.

Received March 12, 2003; returned for revision April 16, 2003; accepted June 9, 2003.

This article has been cited by other articles:

				M. Aluru, Y. Xu, R. Guo, Z. Wang, S. Li, W. White, K. Wang, and S. Rodermel Generation of transgenic maize with enhanced provitamin A content J. Exp. Bot., October 1, 2008; 59(13): 3551 - 3562. [Abstract] [Full Text] [PDF]

				R. Welsch, F. Wust, C. Bar, S. Al-Babili, and P. Beyer A Third Phytoene Synthase Is Devoted to Abiotic Stress-Induced Abscisic Acid Formation in Rice and Defines Functional Diversification of Phytoene Synthase Genes Plant Physiology, May 1, 2008; 147(1): 367 - 380. [Abstract] [Full Text] [PDF]

				R. Welsch, D. Maass, T. Voegel, D. DellaPenna, and P. Beyer Transcription Factor RAP2.2 and Its Interacting Partner SINAT2: Stable Elements in the Carotenogenesis of Arabidopsis Leaves Plant Physiology, November 1, 2007; 145(3): 1073 - 1085. [Abstract] [Full Text] [PDF]

				S. Al-Babili, T. T. C. Hoa, and P. Schaub Exploring the potential of the bacterial carotene desaturase CrtI to increase the {beta}-carotene content in Golden Rice J. Exp. Bot., March 1, 2006; 57(4): 1007 - 1014. [Abstract] [Full Text] [PDF]

				H. Belefant-Miller, G. H. Miller, and J. N. Rutger Nondestructive Measurement of Carotenoids in Plant Tissues by Fluorescence Quenching Crop Sci., August 1, 2005; 45(5): 1786 - 1789. [Abstract] [Full Text] [PDF]

				M. R. Bellon and J. Berthaud Transgenic Maize and the Evolution of Landrace Diversity in Mexico. The Importance of Farmers' Behavior Plant Physiology, March 1, 2004; 134(3): 883 - 888. [Full Text] [PDF]