First published online August 7, 2003; 10.1104/pp.103.026674
Plant Physiology 133:182-190 (2003)
© 2003 American Society of Plant Biologists
CELL BIOLOGY AND SIGNAL TRANSDUCTION
Functional Characterization and Expression Analyses of the Glucose-Specific AtSTP9 Monosaccharide Transporter in Pollen of Arabidopsis1
Alexander Schneidereit,
Joachim Scholz-Starke and
Michael Büttner*
Molekulare Pflanzenphysiologie, Universität Erlangen-Nürnberg, Staudtstrasse 5, D-91058 Erlangen, Germany
A genomic clone and the corresponding cDNA of a new Arabidopsis monosaccharide transporter AtSTP9 were isolated. Transport analysis of the expressed protein in yeast showed that AtSTP9 is an energy-dependent, uncoupler-sensitive, high-affinity monosaccharide transporter with a Km for glucose in the micromolar range. In contrast to all previously characterized monosaccharide transporters, AtSTP9 shows an unusual specificity for glucose. Reverse transcriptase-polymerase chain reaction analyses revealed that AtSTP9 is exclusively expressed in flowers, and a more detailed approach using AtSTP9 promoter/reporter plants clearly showed that AtSTP9 expression is restricted to the male gametophyte. AtSTP9 expression is not found in other floral organs or vegetative tissues. Further localization on the cellular level using a specific antibody revealed that in contrast to the early accumulation of AtSTP9 transcripts in young pollen, the AtSTP9 protein is only found weakly in mature pollen but is most prominent in germinating pollen tubes. This preloading of pollen with mRNAs has been described for genes that are essential for pollen germination and/or pollen tube growth. The pollen-specific expression found for AtSTP9 is also observed for other sugar transporters and indicates that pollen development and germination require a highly regulated supply of sugars.
1 This work was supported by the Deutsche Forschungsgemeinschaft (grant no. Bu 973/4-1).
* Corresponding author; e-mail mbuettne{at}biologie.uni-erlangen.de; fax 49-9131/852—8751.
Received May 9, 2003;
returned for revision June 10, 2003;
accepted June 17, 2003.
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