Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online August 7, 2003; 10.1104/pp.103.023572

Plant Physiology 133:84-99 (2003)
© 2003 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
133/1/84    most recent
pp.103.023572v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (75)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roessner-Tunali, U.
Right arrow Articles by Fernie, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roessner-Tunali, U.
Right arrow Articles by Fernie, A. R.
Agricola
Right arrow Articles by Roessner-Tunali, U.
Right arrow Articles by Fernie, A. R.
BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES

Metabolic Profiling of Transgenic Tomato Plants Overexpressing Hexokinase Reveals That the Influence of Hexose Phosphorylation Diminishes during Fruit Development

Ute Roessner-Tunali, Björn Hegemann, Anna Lytovchenko, Fernando Carrari, Claudia Bruedigam, David Granot and Alisdair R. Fernie*

Department of Lothar Willmitzer, Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany (U.R.-T., B.H., A.L., F.C., C.B., A.R.F.); and Institute for Field and Garden Crops, Agricultural Research Organization, Volcani Centre, Bet Dagan 50250, Israel (D.G.)

We have conducted a comprehensive metabolic profiling on tomato (Lycopersicon esculentum) leaf and developing fruit tissue using a recently established gas chromatography-mass spectrometry profiling protocol alongside conventional spectrophotometric and liquid chromatographic methodologies. Applying a combination of these techniques, we were able to identify in excess of 70 small-Mr metabolites and to catalogue the metabolite composition of developing tomato fruit. In addition to comparing differences in metabolite content between source and sink tissues of the tomato plant and after the change in metabolite pool sizes through fruit development, we have assessed the influence of hexose phosphorylation through fruit development by analyzing transgenic plants constitutively overexpressing Arabidopsis hexokinase AtHXK1. Analysis of the total hexokinase activity in developing fruits revealed that both wild-type and transgenic fruits exhibit decreasing hexokinase activity with development but that the relative activity of the transgenic lines with respect to wild type increases with development. Conversely, both point-by-point and principal component analyses suggest that the metabolic phenotype of these lines becomes less distinct from wild type during development. In summary, the data presented in this paper demonstrate that the influence of hexose phosphorylation diminishes during fruit development and highlights the importance of greater temporal resolution of metabolism.

* Corresponding author; e-mail fernie{at}mpimp-golm.mpg.de; fax 49-0-331-5678408.

Received March 14, 2003; returned for revision May 9, 2003; accepted May 21, 2003.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Fait, K. Hanhineva, R. Beleggia, N. Dai, I. Rogachev, V. J. Nikiforova, A. R. Fernie, and A. Aharoni
Reconfiguration of the Achene and Receptacle Metabolic Networks during Strawberry Fruit Development
Plant Physiology, October 1, 2008; 148(2): 730 - 750.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Akihiro, S. Koike, R. Tani, T. Tominaga, S. Watanabe, Y. Iijima, K. Aoki, D. Shibata, H. Ashihara, C. Matsukura, et al.
Biochemical Mechanism on GABA Accumulation During Fruit Development in Tomato
Plant Cell Physiol., September 1, 2008; 49(9): 1378 - 1389.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Giraud, L. H.M. Ho, R. Clifton, A. Carroll, G. Estavillo, Y.-F. Tan, K. A. Howell, A. Ivanova, B. J. Pogson, A. H. Millar, et al.
The Absence of ALTERNATIVE OXIDASE1a in Arabidopsis Results in Acute Sensitivity to Combined Light and Drought Stress
Plant Physiology, June 1, 2008; 147(2): 595 - 610.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Mintz-Oron, T. Mandel, I. Rogachev, L. Feldberg, O. Lotan, M. Yativ, Z. Wang, R. Jetter, I. Venger, A. Adato, et al.
Gene Expression and Metabolism in Tomato Fruit Surface Tissues
Plant Physiology, June 1, 2008; 147(2): 823 - 851.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Sienkiewicz-Porzucek, A. Nunes-Nesi, R. Sulpice, J. Lisec, D. C. Centeno, P. Carillo, A. Leisse, E. Urbanczyk-Wochniak, and A. R. Fernie
Mild Reductions in Mitochondrial Citrate Synthase Activity Result in a Compromised Nitrate Assimilation and Reduced Leaf Pigmentation But Have No Effect on Photosynthetic Performance or Growth
Plant Physiology, May 1, 2008; 147(1): 115 - 127.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Jumtee, T. Bamba, A. Okazawa, E. Fukusaki, and A. Kobayashi
Integrated metabolite and gene expression profiling revealing phytochrome A regulation of polyamine biosynthesis of Arabidopsis thaliana
J. Exp. Bot., April 1, 2008; 59(6): 1187 - 1200.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
N. Schauer, Y. Semel, I. Balbo, M. Steinfath, D. Repsilber, J. Selbig, T. Pleban, D. Zamir, and A. R. Fernie
Mode of Inheritance of Primary Metabolic Traits in Tomato
PLANT CELL, March 1, 2008; 20(3): 509 - 523.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Vigeolas, C. Chinoy, E. Zuther, B. Blessington, P. Geigenberger, and C. Domoney
Combined Metabolomic and Genetic Approaches Reveal a Link between the Polyamine Pathway and Albumin 2 in Developing Pea Seeds
Plant Physiology, January 1, 2008; 146(1): 74 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Alhagdow, F. Mounet, L. Gilbert, A. Nunes-Nesi, V. Garcia, D. Just, J. Petit, B. Beauvoit, A. R. Fernie, C. Rothan, et al.
Silencing of the Mitochondrial Ascorbate Synthesizing Enzyme L-Galactono-1,4-Lactone Dehydrogenase Affects Plant and Fruit Development in Tomato
Plant Physiology, December 1, 2007; 145(4): 1408 - 1422.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Studart-Guimaraes, A. Fait, A. Nunes-Nesi, F. Carrari, B. Usadel, and A. R. Fernie
Reduced Expression of Succinyl-Coenzyme A Ligase Can Be Compensated for by Up-Regulation of the {gamma}-Aminobutyrate Shunt in Illuminated Tomato Leaves
Plant Physiology, November 1, 2007; 145(3): 626 - 639.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. G. Forde and P. J. Lea
Glutamate in plants: metabolism, regulation, and signalling
J. Exp. Bot., July 1, 2007; 58(9): 2339 - 2358.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Footitt, D. Dietrich, A. Fait, A. R. Fernie, M. J. Holdsworth, A. Baker, and F. L. Theodoulou
The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis
Plant Physiology, July 1, 2007; 144(3): 1467 - 1480.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
D. Glassop, U. Roessner, A. Bacic, and G. D. Bonnett
Changes in the Sugarcane Metabolome with Stem Development. Are They Related to Sucrose Accumulation?
Plant Cell Physiol., April 1, 2007; 48(4): 573 - 584.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Faurobert, C. Mihr, N. Bertin, T. Pawlowski, L. Negroni, N. Sommerer, and M. Causse
Major Proteome Variations Associated with Cherry Tomato Pericarp Development and Ripening
Plant Physiology, March 1, 2007; 143(3): 1327 - 1346.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J.-W. de Kraker, K. Luck, S. Textor, J. G. Tokuhisa, and J. Gershenzon
Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine
Plant Physiology, February 1, 2007; 143(2): 970 - 986.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
V. Joshi, K. M. Laubengayer, N. Schauer, A. R. Fernie, and G. Jander
Two Arabidopsis Threonine Aldolases Are Nonredundant and Compete with Threonine Deaminase for a Common Substrate Pool
PLANT CELL, December 1, 2006; 18(12): 3564 - 3575.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Carrari, C. Baxter, B. Usadel, E. Urbanczyk-Wochniak, M.-I. Zanor, A. Nunes-Nesi, V. Nikiforova, D. Centero, A. Ratzka, M. Pauly, et al.
Integrated Analysis of Metabolite and Transcript Levels Reveals the Metabolic Shifts That Underlie Tomato Fruit Development and Highlight Regulatory Aspects of Metabolic Network Behavior
Plant Physiology, December 1, 2006; 142(4): 1380 - 1396.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Fait, R. Angelovici, H. Less, I. Ohad, E. Urbanczyk-Wochniak, A. R. Fernie, and G. Galili
Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches
Plant Physiology, November 1, 2006; 142(3): 839 - 854.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
U. Roessner, J. H. Patterson, M. G. Forbes, G. B. Fincher, P. Langridge, and A. Bacic
An Investigation of Boron Toxicity in Barley Using Metabolomics
Plant Physiology, November 1, 2006; 142(3): 1087 - 1101.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. W. Davey, K. Kenis, and J. Keulemans
Genetic Control of Fruit Vitamin C Contents
Plant Physiology, September 1, 2006; 142(1): 343 - 351.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Yasuor, M. Abu-Abied, E. Belausov, A. Madmony, E. Sadot, J. Riov, and B. Rubin
Glyphosate-Induced Anther Indehiscence in Cotton Is Partially Temperature Dependent and Involves Cytoskeleton and Secondary Wall Modifications and Auxin Accumulation
Plant Physiology, August 1, 2006; 141(4): 1306 - 1315.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M.-R. Hajirezaei, S. Biemelt, M. Peisker, A. Lytovchenko, A. R. Fernie, and U. Sonnewald
The influence of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) on potato tuber metabolism
J. Exp. Bot., July 1, 2006; 57(10): 2363 - 2377.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Wagner, A. Bernhardt, J. E. Leuendorf, C. Drewke, A. Lytovchenko, N. Mujahed, C. Gurgui, W. B. Frommer, E. Leistner, A. R. Fernie, et al.
Analysis of the Arabidopsis rsr4-1/pdx1-3 Mutant Reveals the Critical Function of the PDX1 Protein Family in Metabolism, Development, and Vitamin B6 Biosynthesis
PLANT CELL, July 1, 2006; 18(7): 1722 - 1735.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Carrari and A. R. Fernie
Metabolic regulation underlying tomato fruit development
J. Exp. Bot., June 1, 2006; 57(9): 1883 - 1897.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Tieman, M. Taylor, N. Schauer, A. R. Fernie, A. D. Hanson, and H. J. Klee
Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde
PNAS, May 23, 2006; 103(21): 8287 - 8292.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Tikunov, A. Lommen, C.H. R. de Vos, H. A. Verhoeven, R. J. Bino, R. D. Hall, and A. G. Bovy
A Novel Approach for Nontargeted Data Analysis for Metabolomics. Large-Scale Profiling of Tomato Fruit Volatiles
Plant Physiology, November 1, 2005; 139(3): 1125 - 1137.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Ishizaki, T. R. Larson, N. Schauer, A. R. Fernie, I. A. Graham, and C. J. Leaver
The Critical Role of Arabidopsis Electron-Transfer Flavoprotein:Ubiquinone Oxidoreductase during Dark-Induced Starvation
PLANT CELL, September 1, 2005; 17(9): 2587 - 2600.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. V. Davies, L. V. T. Shepherd, M. M. Burrell, F. Carrari, E. Urbanczyk-Wochniak, A. Leisse, R. D. Hancock, M. Taylor, R. Viola, H. Ross, et al.
Modulation of Fructokinase Activity of Potato (Solanum tuberosum) Results in Substantial Shifts in Tuber Metabolism
Plant Cell Physiol., July 1, 2005; 46(7): 1103 - 1115.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. A. Garoosi, M. G. Salter, M. X. Caddick, and A. B. Tomsett
Characterization of the ethanol-inducible alc gene expression system in tomato
J. Exp. Bot., June 1, 2005; 56(416): 1635 - 1642.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. J. Baxter, M. Sabar, W. P. Quick, and L. J. Sweetlove
Comparison of changes in fruit gene expression in tomato introgression lines provides evidence of genome-wide transcriptional changes and reveals links to mapped QTLs and described traits
J. Exp. Bot., June 1, 2005; 56(416): 1591 - 1604.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. G. Desbrosses, J. Kopka, and M. K. Udvardi
Lotus japonicus Metabolic Profiling. Development of Gas Chromatography-Mass Spectrometry Resources for the Study of Plant-Microbe Interactions
Plant Physiology, April 1, 2005; 137(4): 1302 - 1318.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
C. J. Baxter, F. Carrari, A. Bauke, S. Overy, S. A. Hill, P. W. Quick, A. R. Fernie, and L. J. Sweetlove
Fruit Carbohydrate Metabolism in an Introgression Line of Tomato with Increased Fruit Soluble Solids
Plant Cell Physiol., March 1, 2005; 46(3): 425 - 437.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Nunes-Nesi, F. Carrari, A. Lytovchenko, A. M.O. Smith, M. Ehlers Loureiro, R. G. Ratcliffe, L. J. Sweetlove, and A. R. Fernie
Enhanced Photosynthetic Performance and Growth as a Consequence of Decreasing Mitochondrial Malate Dehydrogenase Activity in Transgenic Tomato Plants
Plant Physiology, February 1, 2005; 137(2): 611 - 622.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Krishnan, N. J. Kruger, and R. G. Ratcliffe
Metabolite fingerprinting and profiling in plants using NMR
J. Exp. Bot., January 1, 2005; 56(410): 255 - 265.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Urbanczyk-Wochniak and A. R. Fernie
Metabolic profiling reveals altered nitrogen nutrient regimes have diverse effects on the metabolism of hydroponically-grown tomato (Solanum lycopersicum) plants
J. Exp. Bot., January 1, 2005; 56(410): 309 - 321.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. Schauer, D. Zamir, and A. R. Fernie
Metabolic profiling of leaves and fruit of wild species tomato: a survey of the Solanum lycopersicum complex
J. Exp. Bot., January 1, 2005; 56(410): 297 - 307.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. L. Jeong, H. Jiang, H.-S. Chen, C.-J. Tsai, and S. A. Harding
Metabolic Profiling of the Sink-to-Source Transition in Developing Leaves of Quaking Aspen
Plant Physiology, October 1, 2004; 136(2): 3364 - 3375.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists