First published online August 21, 2003; 10.1104/pp.103.026005
Plant Physiology 133:571-579 (2003)
© 2003 American Society of Plant Biologists
CELL BIOLOGY AND SIGNAL TRANSDUCTION
The G-Protein-Coupled Receptor GCR1 Regulates DNA Synthesis through Activation of Phosphatidylinositol-Specific Phospholipase C
Fabio Apone,
Nicole Alyeshmerni,
Kathryn Wiens,
Derek Chalmers,
Maarten J. Chrispeels and
Gabriella Colucci*
Arena Pharmaceuticals, 6166 Nancy Ridge Drive, San Diego, California 92121 (F.A., N.A., K.W., D.C., G.C.); and Division of Biological Sciences, University of California, La Jolla, California 92093–0116 (M.J.C.)
Different lines of evidence suggest that specific events during the cell cycle may be mediated by a heterotrimeric G-protein activated by a cognate G-protein coupled receptor. However, coupling between the only known G -subunit of the heterotrimeric G-protein (GPA1) and the only putative G-protein coupled receptor (GCR1) of plants has never been shown. Using a variety of approaches, we show here that GCR1-enhanced thymidine incorporation into DNA depends on an increase in phosphatidylinositol-specific phospholipase C activity and an elevation of inositol 1,4,5-trisphosphate levels in the cells. Tobacco (Nicotiana tabacum) cells that overexpress either Arabidopsis GCR1 or GPA1 display this phenomenon. We suggest on the basis of these results that GCR1-controlled events during the cell cycle involve phosphatidylinositol-specific phospholipase C as an effector of GCR1 and inositol 1,4,5-trisphosphate as a second messenger, and that GCR1 and GPA1 are both involved in this particular signaling pathway.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.026005.
* Corresponding author; e-mail gcolucci{at}arenapharm.com; fax 858–677–0505.
Received April 24, 2003;
returned for revision May 18, 2003;
accepted June 11, 2003.
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