This Article Full Text Full Text (PDF) All Versions of this Article: 133/2/606    most recent pp.103.025403v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Varotto, S. Articles by Rossi, V. Search for Related Content PubMed PubMed Citation Articles by Varotto, S. Articles by Rossi, V. Agricola Articles by Varotto, S. Articles by Rossi, V.

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Expression Profile and Cellular Localization of Maize Rpd3-Type Histone Deacetylases during Plant Development¹

Dipartimento di Agronomia Ambientale e Produzioni Vegetali-Università di Padova, viale dell'Università 16, I–35020 Legnaro, Padova, Italy (S.V., S.C.); Istituto sperimentale per la Cerealicoltura-Sezione di Bergamo, via Stezzano 24, I–24126 Bergamo, Italy (S.L., M.M., V.R.); and Department of Molecular Biology, University of Innsbruck, Medical School, A–6020 Innsbruck, Austria (A.P.)

We analyzed the expression profile and cellular localization of the maize (Zea mays) Rpd3-type histone deacetylases genes ZmRpd3/101, ZmRpd3/102, and ZmRpd3/108 (indicated as ZmHDA101, ZmHDA102, and ZmHDA108 in the Plant Chromatin Database). This study shows that maize Rpd3 transcripts are present in all the organs and cellular domains analyzed, but we found that their amounts change during development, accumulating in the inner region of the endosperm, in vascular zones of the nucellus, in the tapetum, and in the tetrads. A similar expression profile and nucleus-cytoplasmic localization was observed for ZmRpd3 proteins. Glutathione S-transferase pull-down assays show that ZmRpd3 proteins can interact with the maize retinoblastoma-related (ZmRBR1) protein, an important regulator of cell cycle progression, and with the maize retinoblastoma-associated protein (ZmRbAp1). However, the three ZmRpd3 proteins do not mutually compete in the binding. These results suggest a general role of ZmRpd3 genes in the plant cell cycle and development. These observations also provide indications on possible mechanisms regulating their transcription and protein accumulation. Similarities in the gene expression profiles and protein interactions may indicate that functional redundancy among members of the ZmRpd3 gene family exists. However, a degree of functional divergence is also supported by our findings.

¹ This work was supported by the Framework Program V of the European Community (Zeastar project no. QLRT–2000–00020), by Ministero delle Politiche Agricole (Roma, Italy), and by the Austrian Science Foundation (project no. P14528).

^* Corresponding author; e-mail rossi{at}iscbg.it; fax 39–035–316054.

Received April 17, 2003; returned for revision May 27, 2003; accepted June 26, 2003.

This article has been cited by other articles:

				C. Castro, M. Motto, V. Rossi, and C. Manetti Variation of metabolic profiles in developing maize kernels up- and down-regulated for the hda101 gene J. Exp. Bot., October 10, 2008; (2008) ern239v2. [Abstract] [Full Text] [PDF]

				V. Rossi, S. Locatelli, S. Varotto, G. Donn, R. Pirona, D. A. Henderson, H. Hartings, and M. Motto Maize Histone Deacetylase hda101 Is Involved in Plant Development, Gene Transcription, and Sequence-Specific Modulation of Histone Modification of Genes and Repeats PLANT CELL, April 1, 2007; 19(4): 1145 - 1162. [Abstract] [Full Text] [PDF]

				M. Benhamed, C. Bertrand, C. Servet, and D.-X. Zhou Arabidopsis GCN5, HD1, and TAF1/HAF2 Interact to Regulate Histone Acetylation Required for Light-Responsive Gene Expression PLANT CELL, November 1, 2006; 18(11): 2893 - 2903. [Abstract] [Full Text] [PDF]

				N. Carraro, C. Forestan, S. Canova, J. Traas, and S. Varotto ZmPIN1a and ZmPIN1b Encode Two Novel Putative Candidates for Polar Auxin Transport and Plant Architecture Determination of Maize Plant Physiology, September 1, 2006; 142(1): 254 - 264. [Abstract] [Full Text] [PDF]

				R. Sozzani, C. Maggio, S. Varotto, S. Canova, C. Bergounioux, D. Albani, and R. Cella Interplay between Arabidopsis Activating Factors E2Fb and E2Fa in Cell Cycle Progression and Development Plant Physiology, April 1, 2006; 140(4): 1355 - 1366. [Abstract] [Full Text] [PDF]

				C. Zhou, L. Zhang, J. Duan, B. Miki, and K. Wu HISTONE DEACETYLASE19 Is Involved in Jasmonic Acid and Ethylene Signaling of Pathogen Response in Arabidopsis PLANT CELL, April 1, 2005; 17(4): 1196 - 1204. [Abstract] [Full Text] [PDF]