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First published online September 18, 2003; 10.1104/pp.103.028241

Plant Physiology 133:713-725 (2003)
© 2003 American Society of Plant Biologists

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DEVELOPMENT AND HORMONE ACTION

Transcriptional Profiling of Arabidopsis Tissues Reveals the Unique Characteristics of the Pollen Transcriptome1,[w]

Jörg D. Becker, Leonor C. Boavida, Jorge Carneiro, Matthias Haury and José A. Feijó*

Instituto Gulbenkian de Ciência, PT–2780–156 Oeiras, Portugal (J.D.B., L.C.B., J.C., M.H., J.A.F.); and Centro de Biotecnologia Vegetal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, Ed.C2. PT–1749–016 Lisboa, Portugal (L.C.B., J.A.F.)

Pollen tubes are a good model for the study of cell growth and morphogenesis because of their extreme elongation without cell division. Yet, knowledge about the genetic basis of pollen germination and tube growth is still lagging behind advances in pollen physiology and biochemistry. In an effort to reduce this gap, we have developed a new method to obtain highly purified, hydrated pollen grains of Arabidopsis through flowcytometric sorting, and we used GeneChips (Affymetrix, Santa Clara, CA; representing approximately 8,200 genes) to compare the transcriptional profile of sorted pollen with those of four vegetative tissues (seedlings, leaves, roots, and siliques). We present a new graphical tool allowing genomic scale visualization of the unique transcriptional profile of pollen. The 1,584 genes expressed in pollen showed a 90% overlap with genes expressed in these vegetative tissues, whereas one-third of the genes constitutively expressed in the vegetative tissues were not expressed in pollen. Among the 469 genes enriched in pollen, 162 were selectively expressed, and most of these had not been associated previously with pollen. Their functional classification reveals several new candidate genes, mainly in the categories of signal transduction and cell wall biosynthesis and regulation. Thus, the results presented improve our knowledge of the molecular mechanisms underlying pollen germination and tube growth and provide new directions for deciphering their genetic basis. Because pollen expresses about one-third of the number of genes expressed on average in other organs, it may constitute an ideal system to study fundamental mechanisms of cell biology and, by omission, of cell division.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.028241.

1 This work was supported by the Fundação para a Ciência e a Tecnologia (FCT; project nos. POCTI/BCI/41725/2001, POCTI/BIA/34772/1999, and POCTI/BCI/46453/2002). J.D.B. and L.C.B. were supported by FCT fellowships SFRH/BPD/3619/2000 and SFRH/BD/1128/2000.

[w] The online version of this article contains Web-only data.

* Corresponding author; e-mail jose.feijo{at}fc.ul.pt; fax 351–21–4407970.

Received June 7, 2003; returned for revision July 7, 2003; accepted July 9, 2003.




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