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First published online September 11, 2003; 10.1104/pp.103.026245

Plant Physiology 133:736-747 (2003)
© 2003 American Society of Plant Biologists

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GENETICS, GENOMICS, AND MOLECULAR EVOLUTION

Development of Protoporphyrinogen Oxidase as an Efficient Selection Marker for Agrobacterium tumefaciens-Mediated Transformation of Maize

Xianggan Li*, Sandy L. Volrath1, David B.G. Nicholl, Charles E. Chilcott, Marie A. Johnson, Eric R. Ward1 and Marcus D. Law2

Syngenta Biotechnology, Inc., P.O. Box 12257, 3054 Cornwallis Road, Research Triangle Park, North Carolina 27709–2257

In this article, we report the isolation of plant protoporphyrinogen oxidase (PPO) genes and the isolation of herbicide-tolerant mutants. Subsequently, an Arabidopsis double mutant (Y426M + S305L) was used to develop a selectable marker system for Agrobacterium tumefaciens-mediated transformation of maize (Zea mays) and to obtain multiple events tolerant to the PPO family of herbicides. Maize transformants were produced via butafenacil selection using a flexible light regime to increase selection pressure. Butafenacil selection per se did not change transgene copy number distribution relative to other selectable marker systems, but the most tolerant events identified in the greenhouse were more likely to contain multiple copies of the introduced mutant PPO gene. To date, more than 2,500 independent transgenic maize events have been produced using butafenacil selection. The high frequency of A. tumefaciens-mediated transformation via PPO selection enabled us to obtain single-copy transgenic maize lines tolerant to field levels of butafenacil.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.026245.

1 Present address: Cropsolution, Inc., 120 Southcenter Court, Suite 1000, Morrisville, NC 27560.

2 Present address: BASF Plant Science L.L.C., 26 Davis Drive, Research Triangle Park, NC 27709.

* Corresponding author; e-mail Xianggan.Li{at}syngenta.com; fax 919–541–8585.

Received May 15, 2003; returned for revision July 1, 2003; accepted July 19, 2003.


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