First published online October 2, 2003; 10.1104/pp.103.027847
Plant Physiology 133:1135-1147 (2003)
© 2003 American Society of Plant Biologists
CELL BIOLOGY AND SIGNAL TRANSDUCTION
p-Chlorophenoxyisobutyric Acid Impairs Auxin Response in Arabidopsis Root1
Yutaka Oono*,
Chiharu Ooura2,
Abidur Rahman,
Evalour T. Aspuria,
Ken-ichiro Hayashi,
Atsushi Tanaka and
Hirofumi Uchimiya
Department of Ion-beam-applied Biology (Y.O., A.R., A.T.) and Advanced Science Research Center (Y.O., C.O., E.T.A., H.U.), Japan Atomic Energy Research Institute, Takasaki 370-1292, Japan; Department of Horticulture, University of the Philippines at Los Banos, College, Laguna 4031, Philippines (E.T.A.); Department of Biochemistry, Okayama University of Science, 1–1 Ridai-cho, Okayama 700–0005, Japan (K.-I.H.); and Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113–0032, Japan (H.U.)
p-Chlorophenoxyisobutyric acid (PCIB) is known as a putative antiauxin and is widely used to inhibit auxin action, although the mechanism of PCIB-mediated inhibition of auxin action is not characterized very well at the molecular level. In the present work, we showed that PCIB inhibited BA:: -glucuronidase (GUS) expression induced by indole-3-acetic acid (IAA), 2,4-dichlorophenoxyacetic acid, and 1-naphthaleneacetic acid. PCIB also inhibited auxin-dependent DR5::GUS expression. RNA hybridization and quantitative reverse transcriptase-polymerase chain reaction analyses suggested that PCIB reduced auxin-induced accumulation of transcripts of Aux/IAA genes. In addition, PCIB relieved the reduction of GUS activity in HS::AXR3NT-GUS transgenic line in which auxin inhibits GUS activity by promoting degradation of the AXR3NT-GUS fusion protein. Physiological analysis revealed that PCIB inhibited lateral root production, gravitropic response of roots, and growth of primary roots. These results suggest that PCIB impairs auxin-signaling pathway by regulating Aux/IAA protein stability and thereby affects the auxin-regulated Arabidopsis root physiology.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.027847.
1 A.R. was supported by the Japanese Society for the Promotion of Science Postdoctoral Fellowship for Foreign Researchers, and E.T.A. was supported by the Science and Technology Agency of Japan Nuclear Researchers Exchange Program.
2 Present address: Protein Bio Chip Research Group, Fujirebio Inc., Hachioji, Tokyo 192–0031, Japan.
* Corresponding author; e-mail yoono{at}taka.jaeri.go.jp; fax 81–27–346–9688.
Received May 31, 2003;
returned for revision June 26, 2003;
accepted July 29, 2003.
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