First published online October 9, 2003; 10.1104/pp.103.028266
Plant Physiology 133:1170-1180 (2003)
© 2003 American Society of Plant Biologists
BIOCHEMICAL PROCESSES AND MACROMOLECULAR STRUCTURES
Microspore Separation in the quartet 3 Mutants of Arabidopsis Is Impaired by a Defect in a Developmentally Regulated Polygalacturonase Required for Pollen Mother Cell Wall Degradation1
Seung Y. Rhee,
Erin Osborne,
Patricia D. Poindexter and
Chris R. Somerville*
Carnegie Institution, Department of Plant Biology, 260 Panama Street, Stanford, California 94305 (S.Y.R., E.O., P.P., C.R.S.); and Department of Biological Sciences, Stanford University, Stanford, California 94305 (S.Y.R., C.R.S.)
Mutations in the QUARTET loci in Arabidopsis result in failure of microspore separation during pollen development due to a defect in degradation of the pollen mother cell wall during late stages of pollen development. Mutations in a new locus required for microspore separation, QRT3, were isolated, and the corresponding gene was cloned by T-DNA tagging. QRT3 encodes a protein that is approximately 30% similar to an endopolygalacturonase from peach (Prunus persica). The QRT3 protein was expressed in yeast (Saccharomyces cerevisiae) and found to exhibit polygalacturonase activity. In situ hybridization experiments showed that QRT3 is specifically and transiently expressed in the tapetum during the phase when microspores separate from their meiotic siblings. Immunohistochemical localization of QRT3 indicated that the protein is secreted from tapetal cells during the early microspore stage. Thus, QRT3 plays a direct role in degrading the pollen mother cell wall during microspore development.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.028266.
1 This work was supported in part by the U.S. Department of Energy (grant no. DOE–FG02–00ER20133).
* Corresponding author; e-mail crs{at}andrew2.stanford.edu; fax 650–325–6857.
Received June 7, 2003;
returned for revision July 16, 2003;
accepted August 3, 2003.
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