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First published online October 9, 2003; 10.1104/pp.103.030262

Plant Physiology 133:989-999 (2003)
© 2003 American Society of Plant Biologists

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Reexamining the Role of the Accessory Plasmid pAtC58 in the Virulence of Agrobacterium tumefaciens Strain C581

Gauri R. Nair, Zhenying Liu and Andrew N. Binns*

Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104–6018

Isogenic strains of Agrobacterium tumefaciens carrying pTiC58, pAtC58, or both were constructed and assayed semiquantitatively and quantitatively for virulence and vir gene expression to study the effect of the large 542-kb accessory plasmid, pAtC58, on virulence. Earlier studies indicate that the att (attachment) genes of A. tumefaciens are crucial in the ability of this soil phytopathogen to infect susceptible host plants. Mutations in many att genes, notably attR and attD, rendered the strain avirulent. These genes are located on pAtC58. Previous work also has shown that derivatives of the wild-type strain C58 cured of pAtC58 are virulent as determined by qualitative virulence assays and, hence, pAtC58 was described as nonessential for virulence. We show here that the absence of pAtC58 in pTiC58-containing strains results in reduced virulence but that disruption of the attR gene does not result in avirulence or a reduction in virulence. Our studies indicate that pAtC58 has a positive effect on vir gene induction as revealed by immunoblot analysis of Vir proteins and expression of a PvirB::lacZ fusion.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.030262.

1 This work was supported by the National Science Foundation (grant nos. MCB98–17149 and NIH RO1 GM47369).

* Corresponding author; e-mail abinns{at}sas.upenn.edu; fax 215–898–8780.

Received July 15, 2003; returned for revision August 7, 2003; accepted August 22, 2003.




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