Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online December 4, 2003; 10.1104/pp.103.025759

Plant Physiology 133:1494-1503 (2003)
© 2003 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
133/4/1494    most recent
pp.103.025759v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in Plant Physiol.
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matsumoto, N.
Right arrow Articles by Yamamoto, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsumoto, N.
Right arrow Articles by Yamamoto, N.
Agricola
Right arrow Articles by Matsumoto, N.
Right arrow Articles by Yamamoto, N.

Functional Analysis and Intracellular Localization of Rice Cryptochromes

Nanako Matsumoto, Tomoharu Hirano1, Toshisuke Iwasaki and Naoki Yamamoto*

Graduate School of Humanities and Sciences (N.M.), and Department of Biology (N.Y.), Ochanomizu University, Bunkyo-ku, Tokyo 112–8610, Japan; and Department of Biology, Niigata University, Niigata 950–2181, Japan (T.H., T.I.)

Blue-light-receptor cryptochrome (CRY), which mediates cotyledon expansion, increased accumulation of anthocyanin, and inhibition of hypocotyl elongation, was first identified in Arabidopsis. Two Arabidopsis cryptochromes (AtCRY1 and AtCRY2) have been reported to be localized to the nucleus. However, there is no information on the cryptochromes in monocotyledons. In this study, we isolated two cryptochrome cDNAs, OsCRY1 and OsCRY2, from rice (Oryza sativa) plants. The deduced amino acid sequences of OsCRY1 and OsCRY2 have a photolyase-like domain in their N termini and are homologous to AtCRY1. To investigate the function of OsCRY1, we overexpressed a green fluorescence protein-OsCRY1 fusion gene in Arabidopsis and assessed the phenotypes of the resulting transgenic plants. When the seedlings were germinated in the dark, no discernible effect was observed. However, light-germinated seedlings showed pronounced inhibition of hypocotyl elongation and increased accumulation of anthocyanin. These phenotypes were induced in a blue-light-dependent manner, indicating that OsCRY1 functions as a blue-light-receptor cryptochrome. We also examined the intracellular localization of green fluorescence protein-OsCRY1 in the transgenic plants. It was localized to both the nucleus and the cytoplasm. We identified two nuclear localization domains in the primary structure of OsCRY1. We discuss the relationship between the function and intracellular localization of rice cryptochromes by using additional data obtained with OsCRY2.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.025759.

1 Present address: Graduate School of Frontier Sciences, University of Tokyo, Kashiwa-shi, Chiba 277–8562, Japan.

* Corresponding author; e-mail naoky{at}cc.ocha.ac.jp; fax 81–3–5978–5375.

Received April 18, 2003; returned for revision June 2, 2003; accepted July 5, 2003.


Related articles in Plant Physiol.:

Light Signaling
Edgar P. Spalding
Plant Physiol. 2003 133: 1417-1419. [Full Text]  



This article has been cited by other articles:


Home page
 Plant Physiol.Home page
P. Xu, Y. Xiang, H. Zhu, H. Xu, Z. Zhang, C. Zhang, L. Zhang, and Z. Ma
Wheat Cryptochromes: Subcellular Localization and Involvement in Photomorphogenesis and Osmotic Stress Responses
Plant Physiology, February 1, 2009; 149(2): 760 - 774.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Q. Zhang, H. Li, R. Li, R. Hu, C. Fan, F. Chen, Z. Wang, X. Liu, Y. Fu, and C. Lin
Association of the circadian rhythmic expression of GmCRY1a with a latitudinal cline in photoperiodic flowering of soybean
PNAS, December 30, 2008; 105(52): 21028 - 21033.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Lee, H.-S. Lee, J.-S. Lee, S.-K. Kim, and S.-H. Kim
Hormone- and light-regulated nucleocytoplasmic transport in plants: current status
J. Exp. Bot., September 1, 2008; 59(12): 3229 - 3245.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. Wu and E. P. Spalding
Separate functions for nuclear and cytoplasmic cryptochrome 1 during photomorphogenesis of Arabidopsis seedlings
PNAS, November 20, 2007; 104(47): 18813 - 18818.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
F. Hirose, T. Shinomura, T. Tanabata, H. Shimada, and M. Takano
Involvement of Rice Cryptochromes in De-etiolation Responses and Flowering
Plant Cell Physiol., July 1, 2006; 47(7): 915 - 925.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Chatterjee, P. Sharma, and J. P. Khurana
Cryptochrome 1 from Brassica napus Is Up-Regulated by Blue Light and Controls Hypocotyl/Stem Growth and Anthocyanin Accumulation
Plant Physiology, May 1, 2006; 141(1): 61 - 74.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Jiao, L. Ma, E. Strickland, and X. W. Deng
Conservation and Divergence of Light-Regulated Genome Expression Patterns during Seedling Development in Rice and Arabidopsis
PLANT CELL, December 1, 2005; 17(12): 3239 - 3256.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. D. Platten, E. Foo, R. C. Elliott, V. Hecht, J. B. Reid, and J. L. Weller
Cryptochrome 1 Contributes to Blue-Light Sensing in Pea
Plant Physiology, November 1, 2005; 139(3): 1472 - 1482.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W.A. Rensink and C. R. Buell
Arabidopsis to Rice. Applying Knowledge from a Weed to Enhance Our Understanding of a Crop Species
Plant Physiology, June 1, 2004; 135(2): 622 - 629.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists