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Characterization of a Novel Non-Constitutive Photomorphogenic cop1 Allele¹

Alberts-Ludwigs-Universität Freiburg, Institut für Biologie 2/Botanik, Schänzlestrasse 1, 79104 Freiburg, Germany

A specific light program consisting of multiple treatments with alternating red and far-red light pulses was used to isolate mutants in phytochrome A-dependent signal transduction in Arabidopsis seedlings. Because of their phenotype, the mutants were called eid (empfindlicher im dunkelroten Licht, which means hypersensitive in far-red light). One of the isolated mutants, eid6, is a novel recessive allele of the COP1 gene (constitutive photomorphogenic 1) that carries an amino acid transition in a conserved histidine residue of the RING finger domain. Mutant seedlings exhibited an extreme hypersensitivity towards all tested light qualities, but in contrast to known cop1 alleles, no constitutive photomorphogenic phenotype was detectable in darkness. Thus, the novel cop1^eid6 allele seems to encode for a protein whose remaining activity is sufficient for the suppression of photomorphogenesis in dark-grown plants. In adult cop1^eid6 plants, the development of the Cop1 phenotype is dominated by phytochrome B. Comparison of the phenotype of the novel cop1^eid6 and the weak cop1-4 allele under continuous far-red light indicates that the RING finger and coiled-coil domains of COP1 are sufficient for some specific regulatory function in phytochrome A-dependent high irradiance responses.

¹ This work was supported by the Deutsche Forschungsgemeinschaft (Signaltransduktionsmutanten der Photomorphogenese von Arabidopsis grant to E.S. and T.K.) and by the Fonds der Chemischen Industrie (grant to E.S.).

² Present address: Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, 12 rue de Général Zimmer, 67084 Strasbourg, France.

³ Present address: Deutsches Krebsforschungszentrum, Molekulare Genomanalyse, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.

^* Corresponding author; e-mail thomas.kretsch{at}biologie.unifreiburg.de; fax 49–761–203–2791.

Received June 17, 2003; returned for revision July 11, 2003; accepted August 1, 2003.

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