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First published online November 26, 2003; 10.1104/pp.103.026013

Plant Physiology 133:1768-1778 (2003)
© 2003 American Society of Plant Biologists

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BIOENERGETICS AND PHOTOSYNTHESIS

Decreased Content of Leaf Ferredoxin Changes Electron Distribution and Limits Photosynthesis in Transgenic Potato Plants1

Simone Holtgrefe, Klaus P. Bader, Peter Horton, Renate Scheibe, Antje von Schaewen and Jan E. Backhausen*

Pflanzenphysiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, 9069 Osnabrück, Germany (S.H., R.S., J.E.B.); Fakultät für Biologie, Lehrstuhl für Zellphysiologie, Universität Bielefeld, Postfach 10 01 31, 33501 Bielefeld, Germany (K.P.B.); Robert-Hill Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom (P.H.); and Institut für Botanik, Westfälische Wilhelms-Universität Münster, Schlossgarten 3, 48149 Münster, Germany (A.v.S.)

A complete ferredoxin (Fd) cDNA clone was isolated from potato (Solanum tuberosum L. cv Desiree) leaves. By molecular and immunoblot analysis, the gene was identified as the leaf-specific Fd isoform I. Transgenic potato plants were constructed by introducing the homologous potato fed 1 cDNA clone as an antisense construct under the control of the constitutive cauliflower mosaic virus 35S promoter. Stable antisense lines with Fd contents between 40% and 80% of the wild-type level were selected by northern- and western-blot analysis. In short-term experiments, the distribution of electrons toward their stromal acceptors was altered in the mutant plants. Cyclic electron transport, as determined by the quantum yields of photosystems I and II, was enhanced. The CO2 assimilation rate was decreased, but depending on the remaining Fd content, some lines showed photoinhibition. The leaf protein content remained largely constant, but the antisense plants had a lower total chlorophyll content per unit leaf area and an increased chlorophyll a/b ratio. In the antisense plants, the redox state of the quinone acceptor A in photosystem II (QA) was more reduced than that of the wild-type plants under all experimental conditions. Because the plants with lower Fd amounts reacted as if they were grown under a higher light intensity, the possibility that the altered chloroplast redox state affects light acclimation is discussed.

1 This work was supported by the Deutsche Forschungsgemeinschaft (grant nos. Ba 1864 and FOR 387,TP1) and by the British Council (Academic Research Collaboration).

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.026013.

* Corresponding author; e-mail backhausen{at}biologie.uni-osnabrueck.de; fax 49-541-969-2265.

Received April 24, 2003; returned for revision May 13, 2003; accepted August 14, 2003.




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