First published online October 23, 2003; 10.1104/pp.103.031252
Plant Physiology 133:1809-1819 (2003)
© 2003 American Society of Plant Biologists
DEVELOPMENT AND HORMONE ACTION
An Arabidopsis pex10 Null Mutant Is Embryo Lethal, Implicating Peroxisomes in an Essential Role during Plant Embryogenesis1
Imogen A. Sparkes,
Federica Brandizzi,
Stephen P. Slocombe,
Mahmoud El-Shami2,
Chris Hawes and
Alison Baker*
Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT United Kingdom (I.A.S., S.P.S., M.E., A.B.); and Research School of Biological and Molecular Sciences, Oxford Brookes University, Oxford OX3 0BP United Kingdom (F.B., C.H.)
Peroxisomes participate in many important functions in plants, including seed reserve mobilization, photorespiration, defense against oxidative stress, and auxin and jasmonate signaling. In mammals, defects in peroxisome biogenesis result in multiple system abnormalities, severe developmental delay, and death, whereas in unicellular yeasts, peroxisomes are dispensable unless required for growth of specific substrates. PEX10 encodes an integral membrane protein required for peroxisome biogenesis in mammals and yeast. To investigate the importance of PEX10 in plants, we characterized a Ds insertion mutant in the PEX10 gene of Arabidopsis (AtPEX10). Heterozygous AtPEX10::dissociation element mutants show normal vegetative phenotypes under optimal growth conditions, but produce about 20% abnormal seeds. The embryos in the abnormal seeds are predominantly homozygous for the disruption allele. They show retarded development and some morphological abnormalities. No viable homozygous mutant plants were obtained. AtPEX10 fused to yellow fluorescent protein colocalized with green fluorescent protein-serine-lysine-leucine, a well-documented peroxisomal marker, suggesting that AtPEX10 encodes a peroxisomal protein that is essential for normal embryo development and viability.
1 This work was funded by the Biotechnology and Biological Sciences Research Council (grant no. 24/P13265 to A.B. and a studentship to I.A.S.).
2 Present address: Al-Azhar University, Faculty of Agriculture, Department of Agronomy, Elmokhiam Eldaem Street, Nasser City, Cairo, Egypt.
Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.103.031252.
* Corresponding author; e-mail a.baker{at}leeds.ac.uk; fax 44-113-343-3144.
Received August 1, 2003;
returned for revision August 11, 2003;
accepted August 28, 2003.
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